Colloidal gold immunochromatographic kit for ultra-sensitively detecting Listeria monocytogenes

Abstract

The invention relates to the technical field of immunochromatographic analysis, in particular to a colloidal gold immunochromatographic kit for ultra-sensitively detecting Listeria monocytogenes, which consists of a colloidal gold immunochromatographic test strip and a colorimetric signal amplification reagent, and the test strip is used for detecting Listeria monocytogenes by adopting a double-antibody sandwich method; the colorimetric signal amplification reagent is composed of a gold growth solution A and a gold growth solution B, the gold growth solution A is a coordination growth solutionformed by mixing a polymer and trivalent gold ions (Au3+), and the gold growth solution B is a solution formed by mixing hydroquinone and trisodium citrate. The colloidal gold immunochromatographic kit is simple to operate, high in sensitivity, good in stability, high in detection speed and easy in result reading, does not need other auxiliary instruments and equipment, and can be used for on-site rapid ultra-sensitive detection and large-scale epidemiological investigation of clinical and emergency events.

Description

technical field [0001] The invention relates to the technical field of immunochromatography analysis, in particular to a colloidal gold immunochromatography kit for ultrasensitive detection of Listeria monocytogenes. Background technique [0002] Colloidal gold immunochromatographic test strips have been widely used in clinical diagnosis, food safety testing, and environmental monitoring because of their simplicity, speed, convenience, and naked-eye detection. Traditional colloidal gold immunochromatographic test strips mainly use colloidal gold with a particle size of 20-40nm as the signal probe. However, due to its small particle size, the colorimetric signal intensity is relatively weak, which leads to low detection sensitivity. The relatively low detection sensitivity limits its application in ultrasensitive detection to some extent. In recent years, with the rapid development of nanoscience and technology, various new nanomaterials such as fluorescent microspheres, qua...

AI Technical Summary

Problems solved by technology

[0004] The present invention aims to provide a new technology for improving the detection sensitivity of the traditional colloidal gold immunochromatography method in view of the current defect of the existing colloidal gold immunochromatography test strips, which has low detection sensitivity due to the weak signal strength of the colloidal gold probe. , to achieve on-site rapid detection of ultra-low concentration target detection substances

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