Check patentability & draft patents in minutes with Patsnap Eureka AI!

Method for constructing high-density genotype map of outcrossing species

A high-density gene and genotype technology, applied in the fields of biology, molecular breeding and genetic engineering, can solve the problem of no effective method for identification, and achieve the effect of reducing cost, low cost and accurate positioning

Active Publication Date: 2020-08-07
SHANGHAI NORMAL UNIVERSITY
View PDF3 Cites 5 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There is still no effective method for the identification of important genes for these species

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method for constructing high-density genotype map of outcrossing species
  • Method for constructing high-density genotype map of outcrossing species
  • Method for constructing high-density genotype map of outcrossing species

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0053] In this example, the process of gene mapping in outcrossed diploids will be described in detail.

[0054] In summary, the gene mapping process in outcrossed diploids includes the following steps:

[0055] Step 1: Perform high-coverage sequencing (>20×) on the parents of the target plant, and perform low-coverage sequencing (<5×) on the progeny population of the target plant to obtain corresponding sequencing results.

[0056] Step 2: Align the short-read sequences obtained by sequencing the progeny population (or parent-offspring population) to the existing genome of the target plant, and obtain a variation file containing the variation site.

[0057] Step 3: Based on the sequencing results of the parent materials, screen the SNP sites in the comparison results of all parent-offspring sequencing, and cluster according to the kinship of all offspring groups in the sequenced materials, and then partition the genes. For outcrossed diploids each segment is divided into fou...

Embodiment 2

[0070] In this example, the gene mapping process in double-cross population plants will be described in detail.

[0071] In summary, the gene mapping process in double-cross populations includes the following steps:

[0072] Step 1: Perform high-coverage sequencing (>15×) on the parents of the target plant, and low-coverage sequencing (<1×) on the progeny population of the target plant.

[0073] Step 2: In a similar manner to Example 1, the sequence obtained by sequencing the progeny population is compared with the existing genome.

[0074] Step 3: Based on the sequencing results of the parent material, screen the SNP sites in the comparison results of all parent-offspring sequencing, divide the selected SNP sites into segments, and based on the genetic relationship of the gene segments in each segment after division Perform genotype classification. For the double-cross population, further, the SNP markers were divided into two categories according to the parental genotypes,...

Embodiment 3

[0103] In this example, the gene mapping process in autopolyploid plants will be described in detail.

[0104] In summary, the gene mapping process includes the following steps:

[0105] 1: High-coverage sequencing (>60×) is performed on the parents, and low-coverage sequencing (<5×) is performed on the offspring population (the method of comparing the genome is the same as that of the walnut model).

[0106] 2: the sequence obtained by parent-offspring group sequencing is compared to the existing genome of the target plant, and a variation file is obtained, which contains the gene sequence and variation site information measured by each sequenced material;

[0107] 3: Based on the sequencing results of parental materials, screen the SNP sites in the comparison results of all parent-offspring sequencing, divide the selected SNP sites into segments, and carry out the genetic relationship based on the gene fragments in each segment after division. genotype classification;

[0...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a method for constructing a high-density genotype map of outcrossing species, which comprises the following steps: 1, carrying out high-coverage sequencing on a parent materialof a target plant, and carrying out low-coverage sequencing on a filial generation population material of the target plant to obtain a corresponding sequencing result; 2, comparing sequences obtainedthrough parent-child generation group sequencing to an existing genome of a target plant, and obtaining a variation file, wherein the variation file comprises variation site information measured by each sequenced material; 3, screening single nucleotide polymorphisms (SNP) sites in all parent-child generation sequencing comparison results based on the sequencing results of the parent materials, performing section division on the selected SNP sites, and performing genotype classification based on the genetic relationship of gene segments in each divided section; and 4, carrying out haploid type unification and deletion genotype filling on the divided genotypes.

Description

technical field [0001] The invention relates to the field of biology, in particular to the fields of molecular breeding and genetic engineering. Background technique [0002] With the development of sequencing technology, the application of modern genetic tools has greatly promoted the genetic improvement of various crops. Whole genome sequencing and resequencing are widely used in genetic breeding and mining of agronomic traits. Currently, many genetic research methods focus on plants with self-pollination habits, collected through continuous self-pollination or generating inbred lines for genetic research and breeding. Typical methods include MutMap (1) suitable for analyzing recessive mutant genes induced by EMS and SEG-Map (2) for analyzing self-bred populations. These methods mainly include three steps: 1) sequencing 2) genotyping 3) gene mapping. However, MutMap is only suitable for mutagenesis materials, and SEG-Map is only suitable for genotyping and positioning of...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): G16B30/00G16B20/00
CPCG16B30/00G16B20/00
Inventor 黄学辉杨俊陈蒙娇裴东范维娟王勤张鹏纪飞扬
Owner SHANGHAI NORMAL UNIVERSITY
Features
  • R&D
  • Intellectual Property
  • Life Sciences
  • Materials
  • Tech Scout
Why Patsnap Eureka
  • Unparalleled Data Quality
  • Higher Quality Content
  • 60% Fewer Hallucinations
Social media
Patsnap Eureka Blog
Learn More

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2025 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com