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Composite rhizobium inoculant as well as preparation method and application thereof

A technology of composite rhizobia and rhizobia, applied in the field of agricultural microorganisms, can solve the problems of low plant germination and growth promotion, and achieve the effects of improving nitrogen fixation efficiency, increasing plant growth rate, and promoting growth hormone

Inactive Publication Date: 2020-08-11
ZHENGZHOU UNIVERSITY OF LIGHT INDUSTRY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Cultivated green manure is mainly leguminous crops. All legume green manure crops can nodulate and fix nitrogen with their symbiotic rhizobia. At the same time prevent plant diseases and insect pests from invading

Method used

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  • Composite rhizobium inoculant as well as preparation method and application thereof
  • Composite rhizobium inoculant as well as preparation method and application thereof
  • Composite rhizobium inoculant as well as preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] A compound rhizobia agent, including milk vetch rhizobia, vetch rhizobia, pea rhizobia, clover rhizobia, Frank's actinomycetes, Bacillus subtilis, and Beauveria bassiana;

[0025] The compound rhizobia agent contains milk vetch rhizobia, actinomycetes frankii, rhizobia trifolium, rhizobia trifolium, and rhizobia clover, and the number of effective viable bacteria is 1.5-350 million CFU / g;

[0026] The composite rhizobia agent contains 0.5-250 million CFU / g of viable bacteria of Bacillus subtilis and Beauveria bassiana.

[0027] The preparation method of above-mentioned composite rhizobia agent comprises following preparation steps:

[0028] S1, bacterial agent preparation

[0029] Prepare milk vetch rhizobia agent, vetch rhizobia agent, arrowhead pea rhizobia agent, clover rhizobia agent and Frank's actinomycetes agent respectively, specifically:

[0030] Respectively activate milk vetch rhizobia, vetch rhizobia, pea rhizobia and clover rhizobia, and inoculate them in...

Embodiment 2

[0044] A compound rhizobia agent, including milk vetch rhizobia, vetch rhizobia, pea rhizobia, clover rhizobia, Frank's actinomycetes, Bacillus subtilis, and Beauveria bassiana;

[0045] The compound rhizobia agent contains milk vetch rhizobia, actinomycetes frankii, rhizobia trifolium, rhizobia trifolium, and rhizobia clover, and the number of effective viable bacteria is 200 million CFU / g;

[0046] The composite rhizobia agent contains 180 million CFU / g of Bacillus subtilis and Beauveria bassiana.

[0047] The preparation method of above-mentioned compound rhizobia agent, concrete steps are basically the same as embodiment 1, difference is:

[0048] In S1, the ratio of the inoculum amount of Actinomyces frankii to the inoculation amount of milk vetch rhizobia was 2:1. The culture conditions of the actinomycetes were the same, the culture temperature was 30°C, the rotational speed was 150 rpm, and the culture time was 50 h.

[0049] In S4, the mixing mass ratio of the Bacil...

Embodiment 3

[0051] A compound rhizobia agent, including milk vetch rhizobia, vetch rhizobia, pea rhizobia, clover rhizobia, Frank's actinomycetes, Bacillus subtilis, and Beauveria bassiana;

[0052] The compound rhizobia agent contains milk vetch rhizobia, actinomycetes frankii, rhizobia trifolium, rhizobia trifolium, and rhizobia clover, and the number of effective viable bacteria is 150 million CFU / g;

[0053] The composite rhizobia agent contains both Bacillus subtilis and Beauveria bassiana with a viable count of 50 million CFU / g.

[0054] The preparation method of above-mentioned compound rhizobia agent, concrete steps are basically the same as embodiment 1, difference is:

[0055] In S1, the ratio of the inoculum amount of Actinomyces frankii to the inoculation amount of milk vetch rhizobia was 2:1. The culture conditions of actinomycetes are the same, the culture temperature is 32°C, the rotation speed is 130rpm, and the culture time is 72h.

[0056] In S4, the mixing mass ratio of...

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Abstract

The invention relates to the technical field of agricultural microorganisms, and particularly discloses a composite rhizobium inoculant as well as a preparation method and an application thereof. Thecomposite rhizobium inoculant comprises astragalus sinicus rhizobium, vetch rhizobium, vicia sativa rhizobium, clover rhizobium, Frankia actinomyces, bacillus subtilis and beauveria bassiana. The composite rhizobium inoculant contains astragalus sinicus rhizobium, Frankia actinomycetes, vetch rhizobium, common vetch rhizobium and clover rhizobium, and the effective viable count of the composite rhizobium inoculant is 1.5-3.5 billion CFU / g. The viable count of bacillus subtilis and the viable count of beauveria bassiana contained in the composite rhizobium inoculant are both 0.5 billion to 2.5billion CFU / g. The prepared composite rhizobium inoculant can improve the nitrogen fixation efficiency of leguminous plants, inhibit plant diseases and insect pests and improve the survival rate of crops.

Description

technical field [0001] The invention relates to the technical field of agricultural microorganisms, in particular to a compound rhizobia agent and its preparation method and application. Background technique [0002] Green manure crops are crops that provide crop fertilizer sources and fertilize the soil, such as purple cloud, vetch, arrow pea, clover, alfalfa, sweet clover, tamarind, selenium, amorpha, etc. After the green manure crops have grown for a certain period of time, the green stems and leaves are cut off and directly poured into the soil, which can save manpower, reduce transportation costs, and can also be used as soil fertilizer. Green manure contains a variety of nutrients and a large amount of organic matter, which can improve soil structure, promote soil maturation, and enhance soil fertility. [0003] Nitrogen is the main component of protein, plays an important role in the growth of stems and leaves and the development of fruits, and is the nutrient elemen...

Claims

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Application Information

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IPC IPC(8): A01N63/20A01N63/22A01N63/30A01P3/00A01P1/00A01P7/04C12N1/20C12N1/14C12R1/41C12R1/01C12R1/125C12R1/645A01C1/06
CPCA01C1/06A01N63/20A01N63/22A01N63/30C12N1/14C12N1/20
Inventor 张俊杰孟牒
Owner ZHENGZHOU UNIVERSITY OF LIGHT INDUSTRY
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