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Preparation method of light-controlled tumor pyroptosis system

A tumor cell and nanoparticle technology, applied in the field of nanomedicine, can solve the problems of short blue light wavelength, low skin penetration efficiency, and high phototoxicity, and achieve the effect of avoiding damage

Pending Publication Date: 2020-08-11
TIANJIN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The blue light has a short wavelength, which is very inefficient in penetrating the skin and very phototoxic.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0014] Preparation method of light-controlled tumor cell pyroptosis system:

[0015] 1) Mesoporous silica (mSiO 2 ) Template preparation: ① Weigh an appropriate amount of CTAB and dissolve it in deionized water, add ethanol and NaOH solution. ②Connect the reaction vessel to a heating magnetic stirrer, add tetraethyl orthosilicate (TEOS) dropwise under heating and stirring conditions, and after heating and stirring for a period of time, obtain silicon dioxide nanoparticles (SiO 2 ). ③ SiO 2 Moved into the muffle furnace, calcined at high temperature to remove the CTAB template, to obtain mesoporous silica nanoparticles (mSiO 2 ).

[0016] 2) Preparation of near-infrared excited upconversion long-lasting nanoparticles (mUPLNs) by template method: ① Configure Eu(NO3)2·6H2O and Dy(NO3)2 according to the stoichiometric ratio of Eu / Dy (molar ratio = 1 / 2) The final concentrations of 6H2O, Eu2+ and Dy3+ were controlled at 1mol / L and 2mol / L, respectively, and dissolved in water / et...

Embodiment 2

[0021] 1) Mesoporous silica (mSiO 2 ) Template preparation: ① Weigh an appropriate amount of CTAB and dissolve it in deionized water, add ethanol and NaOH solution. ②Connect the reaction vessel to a heating magnetic stirrer, add tetraethyl orthosilicate (TEOS) dropwise under heating and stirring conditions, and after heating and stirring for a period of time, obtain silicon dioxide nanoparticles (SiO 2 ). ③ SiO 2 Moved into the muffle furnace, calcined at high temperature to remove the CTAB template, to obtain mesoporous silica nanoparticles (mSiO 2 ).

[0022] 2) Preparation of near-infrared excited up-conversion long-lasting nanoparticles (mUPLNs) by template method: ① Configure Eu(NO 3 ) 2 ·6H 2 O and Dy(NO 3 ) 2 ·6H 2 O, Eu2+ and Dy 3+ The final concentrations were controlled at 1mol / L and 2mol / L, respectively, and dissolved in water / ethanol (1 / 1, v / v) to form a precursor solution. ② Mix the precursor solution with the mesoporous silica nanoparticles under vacuu...

Embodiment 3

[0027] 1) Mesoporous silica (mSiO 2 ) Template preparation: ① Weigh an appropriate amount of CTAB and dissolve it in deionized water, add ethanol and NaOH solution. ②Connect the reaction vessel to a heating magnetic stirrer, add tetraethyl orthosilicate (TEOS) dropwise under heating and stirring conditions, and after heating and stirring for a period of time, obtain silicon dioxide nanoparticles (SiO 2 ). ③ SiO 2 Moved into the muffle furnace, calcined at high temperature to remove the CTAB template, to obtain mesoporous silica nanoparticles (mSiO 2 ).

[0028] 2) Preparation of near-infrared excited up-conversion long-lasting nanoparticles (mUPLNs) by template method: ① Configure Eu(NO 3 ) 2 ·6H 2 O and Dy(NO 3 ) 2 ·6H 2 O, Eu 2+ and Dy 3+ The final concentrations were controlled at 1mol / L and 2mol / L, respectively, and dissolved in water / ethanol (1 / 1, v / v) to form a precursor solution. ② Mix the precursor solution with the mesoporous silica nanoparticles under vac...

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Abstract

The invention belongs to the field of nanomedicine and relates to a preparation method of a light-controlled tumor pyroptosis system. An up-conversion long afterglow luminescent material is selected as a light supply factor; and the light-controlled tumor pyroptosis system is combined by using a pyroptosis sensitive plasmid A (Cry2-GSDMD-CAAX) and an accessory protein plasmid B (Cib-Caspase-1). The preparation method of the light-controlled tumor pyroptosis system comprises the steps of (1) preparing a mesoporous silica (mSiO2) template; (2) preparing near-infrared-excited up-conversion long afterglow nanoparticles (mUPLNs) by adopting a template method; (3) modifying a gene targeting delivery vector; (4) establishing precursor genes Cib-GSDMD-CAAX and Cry2-Caspase-1 for light control; and(5) after expression of the plasmids A and B, mixing the plasmids A and B with the up-conversion long afterglow luminescent material exposed to near-infrared light to induce pyroptosis.

Description

technical field [0001] The invention belongs to the field of nanomedicine and relates to a method for controlling pyroptosis of tumor cells by light in vivo. The system can induce pyroptosis of tumor cells under near-infrared irradiation. Background technique [0002] Malignant tumor is an incurable disease with high morbidity and mortality in society. At present, the clinical treatment of tumors can only rely on some treatment methods such as chemotherapy and drug therapy. However, drug treatment and chemotherapy can cause great damage to normal tissues of the human body, and cannot provide controllable treatment for tumors. Pyroptosis, also known as inflammatory necrosis, is a kind of programmed cell death, manifested by continuous swelling of cells until the cell membrane ruptures, resulting in the release of cell contents and activation of a strong inflammatory response. Pyroptosis is an important natural immune response of the body and plays an important role in fight...

Claims

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Application Information

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IPC IPC(8): A61K48/00A61K38/17A61K41/00A61K47/58A61K47/62A61K47/69A61P35/00
CPCA61K48/005A61K48/0008A61K38/1709A61K41/0052A61K47/58A61K47/6949A61K47/62A61P35/00A61K2300/00
Inventor 郑斌郭明明明东甘霖
Owner TIANJIN UNIV
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