Rare earth element labeled graphene oxide nanosheet as well as preparation method and application thereof
A graphene nanosheet and rare earth element technology, applied in the field of biomedicine, can solve the problems of difficult quantitative analysis, limited, weak Raman scattering effect, etc., and achieve the effect of solving difficult detection.
Active Publication Date: 2020-08-11
INST OF HIGH ENERGY PHYSICS CHINESE ACAD OF SCI
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Problems solved by technology
However, since GO is mainly composed of C and O, the quantitative analysis of GO is a difficult point in its application.
[0003] Currently commonly used GO quantification methods include radioactive element tracers (such as 125 I or 111 In or 14 C), fluorescent labeling and Raman spectroscopy, however, radioactive labeling methods are limited by dedicated radiochemical laboratory conditions; the Raman scattering effect is a very weak process, and due to the interference of the fluorescent signal by the complex system of the environment, GO The Raman signal is very weak in complex systems, making it difficult to use for quantitative analysis
Fluorescent molecular labeling method, the fluorescent signal is easily quenched by GO, which cannot meet the detection requirements in complex biological systems
Method used
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Embodiment 1
[0038] Separation and purification of GO
[0039] GO sheets with a diameter of 10-1000 nm were selected as raw materials, and GO nanosheets with a diameter of 70-100 nm were obtained by probe-type ice-bath ultrasound and step-by-step centrifugation.
[0040] The ultrasonic power of the probe-type ultrasonic instrument is 500W, and the working mode is 5s after working for 5s. The total time of ultrasound is 12h.
[0041] The step-by-step centrifugation is three-stage separation, and the rotating speeds are respectively set at 5000-7000rpm, 9000-11000rpm and 12000-14000rpm.
[0042] Carboxylation modification of GO
[0043] Stir 10 mL, 2 mg / mL of the separated and purified GO aqueous dispersion for 12 h, and the rotating speed of the stirring magnet is 500 rpm. Dissolve 1.2 g of sodium hydroxide and 1.0 g of chloroacetic acid in 2 mL of water respectively, ensuring that the pH of the solution is 13.5. The sodium hydroxide solution was cooled to room temperature 22°C and ...
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Abstract
The invention discloses a rare earth element labeled graphene oxide nanosheet. A rare earth element compound is connected with the graphene oxide nanosheet through an amido bond. The invention furtherdiscloses a preparation method and an application of the rare earth element labeled graphene oxide nanosheet. According to the invention, the structural characteristics of graphene oxide (GO) are utilized, wherein the surface of graphene oxide contains rich carboxyl, hydroxyl and epoxy groups; functional modification is carried out; the GO modified by a tetraazacyclo compound is obtained; the rare earth ion labeled GO is obtained by utilizing the strong rare earth element ion complexing capacity of the tetraazacyclo-compound, a convenient and effective method can be provided for quantitativeanalysis of the GO in a complex system by measuring the rare earth ions, and the problem that the graphene oxide is difficult to detect in a biological system is solved.
Description
technical field [0001] The invention belongs to the field of biomedicine, and in particular relates to rare earth element-labeled graphene oxide nanosheets, a preparation method and application thereof. Background technique [0002] Graphene is the thinnest known fully two-dimensional crystalline structure, composed entirely of carbon atoms. In recent years, graphene has broad application prospects in various fields such as industry and biomedicine due to its good electrical, thermal, and chemical properties. Graphene oxide (GO) has good hydrophilicity and biocompatibility, can form a stable suspension in water and other organic solvents, is easy to modify and functionalize, and can be widely used in biosensing, biodetection, pharmaceuticals, etc. fields such as transmission and tumor targeted therapy. However, since GO is mainly composed of C and O, the quantitative analysis of GO is a difficult point in its application. [0003] Currently commonly used GO quantification...
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IPC IPC(8): C01B32/198C01B32/194
CPCC01B32/198C01B32/194C01B2204/32
Inventor 汪冰于宏洋王萌陈汉清郑令娜杜伟丰伟悦
Owner INST OF HIGH ENERGY PHYSICS CHINESE ACAD OF SCI