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Bacteriocin with antibacterial activity on plurality of pathogenic bacteria

A technology of bacteriostatic activity and bacteriocin, applied in the field of microorganisms, can solve problems such as carcinogenesis and teratogenicity

Active Publication Date: 2020-08-11
HUAIBEI NORMAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0002] As people pay more attention to food safety and their own health, traditional fresh-keeping technologies such as chemical preservatives can cause adverse effects on blood pressure, heart, kidneys, etc., and even some have carcinogenicity and teratogenicity, which can no longer meet people's needs. , and finding natural and safe food preservatives has become a research hotspot in the food industry

Method used

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  • Bacteriocin with antibacterial activity on plurality of pathogenic bacteria
  • Bacteriocin with antibacterial activity on plurality of pathogenic bacteria
  • Bacteriocin with antibacterial activity on plurality of pathogenic bacteria

Examples

Experimental program
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Embodiment 1

[0017] 1. Detection of antibacterial activity of Bacillus phaseiyama XIN-1 strain

[0018] Pick a single colony of Bacillus phase alpinus XIN-1, inoculate in 5ml LB liquid medium and activate overnight at 30°C. Transfer 1 mL of the culture to 100 mL of LB liquid medium (recipe: peptone 10 g, yeast powder 5 g, sodium chloride 10 g, add distilled water to 1 L, pH 7.0, sterilize at 121 °C for 30 min), at 220 rpm / min, Incubate at 30°C for 30h. From inoculation, 1 mL of culture solution was taken in the ultra-clean bench every 2 h, and samples were taken 12 times in a row. After the culture solution was centrifuged at 12000rpm / min for 5min, the supernatant was transferred to a new centrifuge tube, and the antibacterial activity of the fermentation supernatant against the indicator bacteria was determined. The antibacterial activity of the fermentation supernatant was tested by agar diffusion method (Cintas et al., 1995). Add an appropriate amount of indicator bacteria (the numbe...

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Abstract

The invention discloses a bacteriocin bacyclin XIN-1 with antibacterial activity on a plurality of pathogenic bacteria and a preparation method of the bacteriocin bacicyclicin XIN-1. The cyclic bacteriocin is a novel cyclic bacteriocin, and the molecular weight is 5848.2032 Da. The nucleotide sequence of a precursor peptide of the cyclic bacteriocin is shown as SEQ ID NO: 1, and the amino acid sequence of the precursor peptide of the cyclic bacteriocin is shown as SEQ ID NO: 2. The bacteriocin bacicyclicin XIN-1 is produced from bacillus alpine XIN-1, and has efficient antibacterial activity on a plurality of pathogenic bacteria such as bacillus cereus, listeria monocytogenes, staphylococcus aureus and the like.

Description

technical field [0001] The invention belongs to the field of microorganisms, and in particular relates to a bacteriocin bacicyclin XIN-1 which has antibacterial activity against various pathogenic bacteria. Background technique [0002] As people pay more attention to food safety and their own health, traditional fresh-keeping techniques such as chemical preservatives can cause adverse effects on blood pressure, heart, kidneys, etc., and even some have carcinogenicity and teratogenicity, which can no longer meet people's needs. , and searching for natural and safe food preservatives has become a research hotspot in the food industry. [0003] Bacteriocins are a class of proteins or polypeptides with antibacterial activity produced by bacteria through the ribosome synthesis mechanism. Bacteriocin itself has many excellent characteristics: high antibacterial activity in vivo and in vitro; non-toxic to humans or animals, and easily degraded by some proteases in the digestive t...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K14/32C12N15/31A61P31/04
CPCC07K14/32A61P31/04Y02A50/30
Inventor 信丙越李峰刘舒曾化伟曾昕王光利张标徐大勇
Owner HUAIBEI NORMAL UNIVERSITY