Preparation method and application of eucommia ulmoides fermentation product

A technology of fermented product and Eucommia ulmoides leaf powder, which can be used in applications, medical preparations containing active ingredients, animal feed, etc., and can solve problems such as few reports.

Inactive Publication Date: 2020-08-14
OCEAN UNIV OF CHINA +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there are few reports about Eucommia ulmoides as a feed additive in marine fish farming.

Method used

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  • Preparation method and application of eucommia ulmoides fermentation product
  • Preparation method and application of eucommia ulmoides fermentation product
  • Preparation method and application of eucommia ulmoides fermentation product

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] Example 1 Determination of Optimal Fermentation Conditions for Eucommia Leaf Powder

[0032] Select McFarland agar medium: take 50 g of McFarland agar powder, add it into an Erlenmeyer flask filled with 1000 mL of distilled water, heat and boil to dissolve and stir, and then sterilize at 121° C. for 15 minutes for later use. Pour it into 8 sterilized and dry petri dishes when it is not cooled and solidified, and wait for the agar to cool and solidify. After cooling, refrigerate at 4°C for later use.

[0033] To prepare MH broth, weigh 30 g of MH broth powder, add it to a conical flask filled with 1000 mL of distilled water, heat to boil, dissolve and stir, and then sterilize at 121°C for 15 minutes before use. After cooling, refrigerate at 4°C for later use.

[0034] Take the cryopreserved plastic cryopreservation tube, immediately place it in a water bath at 38°C to 40°C for rapid recovery and shake it properly. Until the internal ice is completely dissolved, open t...

Embodiment 2

[0038] Example 2 Determination of the optimum amount of fermented eucommia leaf powder in large yellow croaker feed

[0039] 1) Preparation of fermented eucommia leaf powder additive feed

[0040]Fermented eucommia leaf powder was formed by selecting pulverized and dried eucommia leaf powder and fermented. According to the nutritional requirements of large yellow croaker, the experimental formula was designed to make experimental basic materials. Fermented eucommia leaf powder was added to the feed in gradient to prepare different fermented eucommia leaf powder. Added amount of large yellow croaker feed (see Table 2 for the experimental formula of the fermented Eucommia ulmoides leaf additive). The juvenile large yellow croaker used in the experiment has a body length of 8-11 cm.

[0041] Table 2: Experimental formula of fermented Eucommia leaf additive

[0042]

[0043] 2) Feeding growth experiment

[0044] After feeding the large yellow croaker feed with different amou...

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PUM

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Abstract

The present invention provides a preparation method of a eucommia ulmoides fermentation product. The method comprises the steps of mixing eucommia ulmoides leaf powder and a solid strain according tothe mass ratio of 500:1 for fermentation, and uniformly mixing a mixture and water according to the ratio of (3-4):1 by g:ml. The invention further discloses an application of the eucommia ulmoides fermentation product in preparation of aquatic feed and the pseudosciaena crocea feed. By adding the low-content eucommia ulmoides fermentation product into the feed, the growth performance of pseudosciaena crocea can be improved by enhancing the oxidation resistance and immunity of the pseudosciaena crocea and improving the intestinal health.

Description

technical field [0001] The invention belongs to the technical field of aquaculture, and in particular relates to a preparation method of a fermented product containing Eucommia ulmoides and its application in preparing aquatic feed. Background technique [0002] Large yellow croaker, commonly known as "yellow croaker", is the marine fish with the largest breeding output in my country. In 2018, the domestic output was close to 200,000 tons, which has huge economic value. In actual production, it has been found that large yellow croaker farming is prone to problems such as slow growth and decreased immunity, and many problems such as abnormal fat deposition, decreased immunity and inflammatory reactions are prone to occur during the breeding process of large yellow croaker. solve. [0003] Eucommia ulmoides Oliv. belongs to Eucommia ulmoides and the genus Eucommia, and is a unique tree species in my country. It has the effects of lowering blood pressure, lowering fat and blo...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23K50/80A23K10/12A23K10/30A23K10/22A23K20/147A23K20/158A61K36/46A61K9/14A61P37/04A61P39/06A61P1/00A61P29/00A61P3/00A61K127/00
CPCA23K50/80A23K10/12A23K10/30A23K10/22A23K20/147A23K20/158A61K36/46A61K9/14A61P37/04A61P39/06A61P1/00A61P29/00A61P3/00A61K2236/19Y02A40/818
Inventor 艾庆辉郑修坤于伟徐建伟李庆飞王震麦康森林伟东徐玮
Owner OCEAN UNIV OF CHINA
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