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Traditional Chinese medicinal compound chicken Newcastle disease vaccine and preparation method thereof

A technology of chicken Newcastle disease and chicken Newcastle disease virus, which is applied in the field of traditional Chinese medicine compound chicken Newcastle disease vaccine and its preparation, can solve the problems of cumbersome extraction process, unstable effect, and limited development, achieve high antibody titer, stable immune effect, and simple and easy preparation method Effect

Active Publication Date: 2020-08-14
SHANDONG LVDU BIO SICIENCE & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although the polysaccharide adjuvant is safe, effective and reliable, it is a promising adjuvant, but its extraction process is cumbersome, costly, and its effect is unstable, which limits its development.

Method used

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  • Traditional Chinese medicinal compound chicken Newcastle disease vaccine and preparation method thereof
  • Traditional Chinese medicinal compound chicken Newcastle disease vaccine and preparation method thereof
  • Traditional Chinese medicinal compound chicken Newcastle disease vaccine and preparation method thereof

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preparation example Construction

[0023] The invention provides a preparation method of a traditional Chinese medicine compound chicken Newcastle disease vaccine, comprising the steps of:

[0024] (1) Preparation of traditional Chinese medicine compound adjuvant: the raw materials of the traditional Chinese medicine compound adjuvant include astragalus, hawthorn, bergamot, agrimony, perilla and licorice;

[0025] mixing and pulverizing the raw materials to obtain traditional Chinese medicine powder;

[0026] soaking the traditional Chinese medicine powder in water to obtain an extract;

[0027] Concentrate the extract, add ethanol to precipitate, and obtain the traditional Chinese medicine precipitate, which is the traditional Chinese medicine compound adjuvant.

[0028] (2) The cell culture liquid of chicken Newcastle disease virus is mixed with the traditional Chinese medicine compound adjuvant, and freeze-dried to obtain the Chinese medicine compound chicken Newcastle disease vaccine.

[0029] The inventi...

Embodiment 1

[0055] Weigh 16 parts of astragalus, 13 parts of hawthorn, 8 parts of bergamot, 10 parts of Agrimony, 13 parts of perilla and 9 parts of licorice, which are washed and dried, mix and pulverize, pass through a 30-mesh sieve, and take the sieve to obtain Chinese medicine powder; Add 3 times of distilled water to soak for 4 hours at a weight ratio of 1:3, and perform the first ultrasonic treatment after soaking for 30 minutes. The power of the ultrasonic treatment is 180 watts, and the ultrasonic treatment is 20 minutes. The power is 180 watts, ultrasonic treatment for 20 minutes, continue to soak, after soaking, filter the soaking solution to obtain the extract; heat and concentrate the extract to 1 / 5 of the original volume, and then add 75% of the volume of the concentrated extract to 3 times the volume Precipitate with ethanol and let it stand for 45 minutes; centrifuge to obtain the precipitate of traditional Chinese medicine, which is the compound adjuvant of traditional Chin...

Embodiment 2

[0058] Weigh 14 parts of astragalus, 10 parts of hawthorn, 9 parts of bergamot, 11 parts of agrimony, 14 parts of perilla and 12 parts of licorice, which are washed and dried, mix and pulverize, pass through a 20-mesh sieve, and take the sieve to obtain a Chinese medicine powder; The weight ratio is 1:2, add 2 times of distilled water to soak for 5 hours, and perform the first ultrasonic treatment after soaking for 30 minutes. The power of the ultrasonic treatment is 150 watts, and the ultrasonic treatment is 30 minutes. The power is 180 watts, ultrasonic treatment for 30 minutes, continue to soak, after soaking, filter the soaking solution to obtain the extract; heat and concentrate the extract to 1 / 4 of the original volume, and then add 80% of the volume of the concentrated extract to 4 times the volume Precipitate with ethanol and let it stand for 30 minutes; centrifuge to obtain the precipitate of traditional Chinese medicine, which is the compound adjuvant of traditional C...

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Abstract

The invention belongs to the technical field of biological vaccine products for livestock and poultry and provides a traditional Chinese medicinal compound chicken Newcastle disease vaccine and a preparation method thereof. The preparation method comprises the steps of mixing a chicken Newcastle disease cell culture fluid with a traditional Chinese medicinal compound adjuvant, performing freezingand drying, and performing grinding to obtain the traditional Chinese medicinal compound chicken Newcastle disease vaccine. The traditional Chinese medicinal compound adjuvant is prepared from the following raw materials: astragalus roots, haws, fingered citron, herba agrimoniae, purple perilla and licorice roots; the raw materials are mixed and ground to obtain traditional Chinese medicinal powder; the traditional Chinese medicinal powder is soaked with water to obtain an extract; the extract is concentrated and precipitated in alcohol to obtain traditional Chinese medicinal precipitate, namely the traditional Chinese medicinal compound adjuvant. The traditional Chinese medicinal compound chicken Newcastle disease vaccine can be used for inducing a chicken body to generate high antibody titer, can rapidly generate antibodies and has long lasting time and stable immune effect, and the preparation method is simple and feasible.

Description

technical field [0001] The invention relates to the technical field of vaccine products, in particular to a traditional Chinese medicine compound chicken Newcastle disease vaccine and a preparation method thereof. Background technique [0002] Newcastle disease is an acute, febrile, highly contagious infectious disease caused by Newcastle Disease Virus (NDV), which mainly affects chickens and turkeys, but can also cause ducks, geese and other waterfowl to be infected. The disease is a serious hazard. One of the important viral diseases in poultry farming. NDV is a single-stranded negative-sense RNA virus belonging to the family Paramyxoviridae and the genus Avulavirus. In recent years, with the change of breeding scale and breeding environment, the virulence and host range of NDV are also changing and developing. [0003] At present, the prevention and control of Newcastle disease in my country mainly relies on vaccine immunization to prevent it. However, modern vaccines ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K39/39A61K39/17A61P31/14
CPCA61K39/39A61K39/12A61P31/14A61K2039/55588A61K2039/552A61K2039/522A61K2039/5252C12N2760/18134Y02A50/30
Inventor 董炳梅沈志强管宇唐娜苗立中
Owner SHANDONG LVDU BIO SICIENCE & TECH