Method for detecting carbamazepine blood drug concentration by liquid chromatography-mass spectrometry

A carbamazepine and liquid chromatography technology, which is applied in the field of detection of carbamazepine blood drug concentration, can solve the problems of limited detection and achieve the effects of reduced detection cost, high resolution and sensitivity, and strong specificity

Inactive Publication Date: 2020-08-14
WUXI APPTEC ZK SUZHOU BIOSCIENCE CO LTD
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Problems solved by technology

[0007] The purpose of the present invention is to solve the problem that the matrix of the existing carbamazepine blood drug concentration needs to use the human source matrix to cause limited detection in the detection process of the existing carbamazepine blood drug concentration. A detection method using animal serum instead of human serum as a matrix for TDM detection

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  • Method for detecting carbamazepine blood drug concentration by liquid chromatography-mass spectrometry
  • Method for detecting carbamazepine blood drug concentration by liquid chromatography-mass spectrometry
  • Method for detecting carbamazepine blood drug concentration by liquid chromatography-mass spectrometry

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Embodiment

[0044] Example: the assay method of carbamazepine plasma concentration in serum:

[0045] 1. Preparation method of calibrator solution and quality control solution:

[0046] 1. Carbamazepine stock solution: take 10 mg of carbamazepine, accurately weigh it, put it in a 10 mL volumetric flask, add an appropriate amount of methanol to dissolve and dilute to the mark, mix well, and prepare a carbamazepine containing 1.0 mg of carbamazepine per mL Xiping stock solution;

[0047] 2. Internal standard working solution: take 0.2 mL of D10-carbamazepine methanol solution (100 μg / mL) (Sigma), add 500 mL of methanol-water (80:20, v:v), and mix well.

[0048] 3. Calibrator solution:

[0049] 3.1. A certain amount of carbamazepine stock solution was added to animal serum to prepare SC-H solution containing 20 μg of carbamazepine per ml and SC-L solution containing 0.5 μg of carbamazepine per ml.

[0050] 3.2. Mix SC-H and SC-L solutions in different proportions to prepare carbamazepine ca...

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Abstract

The invention relates to a method for detecting carbamazepine blood drug concentration by liquid chromatography-mass spectrometry. The method comprises the following specific steps: preparing a carbamazepine stock solution; preparing a calibration product solution; preparing a quality control product solution; pretreating a to-be-detected sample; setting liquid chromatography-mass spectrometry experimental parameters; performing liquid chromatography tandem mass spectrometry analysis on the treated to-be-detected serum sample according to 2microliters of the sample; and finally calculating theconcentration of carbamazepine in the serum sample. The matrixes of the standard substance solution and the quality control substance solution use the animal serum to replace human serum, the detection cost is reduced, and the method for detecting the concentration of carbamazepine in serum is accurate, rapid and high in specificity.

Description

technical field [0001] The invention relates to a method for detecting the blood drug concentration of carbamazepine by liquid chromatography-mass spectrometry, and belongs to the technical field of analytical chemistry. Background technique [0002] Carbamazepine is a highly effective broad-spectrum antiepileptic drug that can treat a variety of epilepsy such as partial seizures, complex partial seizures, simple partial seizures, secondary generalized seizures, generalized seizures, and tonic-paroxysmal seizures. It has curative effect on symptoms such as seizures, and has a certain therapeutic effect on neuropathic pain, arrhythmia, prevention and treatment of manic depression. [0003] The plasma concentration of carbamazepine is directly related to the dosage, clinical efficacy and side effects. The recommended plasma concentration of carbamazepine is 5 to 10 μg / mL. Overdose may cause severe dizziness, drowsiness, irregular breathing, tremors, abnormal heartbeat, coma,...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02G01N30/06G01N30/30G01N30/32G01N30/34G01N30/72
CPCG01N30/02G01N30/06G01N30/30G01N30/32G01N30/34G01N30/72G01N2030/324
Inventor 盛蔚丁沛瑜王金玉
Owner WUXI APPTEC ZK SUZHOU BIOSCIENCE CO LTD
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