Application of melatonin in the preparation of drugs for inhibiting and/or killing bacteria

A technology of melatonin and bacteria, applied in the field of medicine, can solve the problems of bacteria and bacterial infectious diseases that have not yet been reported, and achieve the effects of improving survival, reducing lesions, and inhibiting colonization

Active Publication Date: 2021-03-16
SOUTH CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

But so far, the study of melatonin inhibiting bacteria

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  • Application of melatonin in the preparation of drugs for inhibiting and/or killing bacteria
  • Application of melatonin in the preparation of drugs for inhibiting and/or killing bacteria
  • Application of melatonin in the preparation of drugs for inhibiting and/or killing bacteria

Examples

Experimental program
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Example Embodiment

[0107] Example 1 Test of melatonin broad-spectrum antibacterial and / or sterilization

[0108] Bacteria suspension that grows 1ML of fresh alignment (1 × 10 9 CFUs were added to 100 mL, respectively, containing different concentrations of melatonin (50 mg / ml, 25 mg / ml, 12.5 mg / ml, 6.25 mg / ml, 3.125 ml / ml, 1.5625 mg / ml, 0.78125mg / ml, 0.390625 mg / ml) The DMSO dilution containing no melatonin (MT) was used as the control group, and the detection of Mt on the minimum antibacterial concentration MIC of bacteria was performed at 37 ° C, 120 r / min. Then, 0.1 ml of the above-mentioned transparent bacterial solution was absorbed onto the sterilized specific medium agar plate and the coating was uniform, incubated in a 37 ° C incubator for 24 hours, and the minimum bactericidal concentration MBC of the pathogenesis was performed, wherein there were no bacteria The lowest concentration of growth is MT to the MBC of pathogen. Each experiment was carried out 3 times, and 3 r...

Example Embodiment

[0114] Example 2 Antibacterial activity test of melatonin on bacteria

[0115] In order to further verify the antibacterial activity of the hyperincardin, the bacterial growth curve is determined in the case of melatonin concentration. Pasteliobacterium in 1 ml of alternatives (1.0 × 10 9 CFU) Add 100 ml of fresh sterile martic broth culture medium, which contains a MT concentration below MBC (1 mg / ml, 1.5 mg / ml and 2 mg / ml), and DMSO containing free melanin as a solvent. Control group. The bacteria were then cultured in a thermostatic closing of 37 ° C, and the OD600 of the primary bacterial culture was determined using an ultraviolet spectrophotometer to detect the effect of melatonin on bacterial growth curve. Each experiment was carried out three times and 3 repetitions per group.

[0116] by figure 1 The results show that melatonin can significantly inhibit bacterial growth.

Example Embodiment

[0117] Example 3 Melatonin killing bacteria

[0118] 1 ml of multi-killing Bacillus for several long-term periods (1 × 10 9 CFU) Add 100 ml of fresh sterile martial broth culture medium. The bacteria were cultured in a thermostatic shaker at 37 ° C, 220 rpm. Subsequently, different concentrations of MT (4 mg / ml, 8 mg / ml, and 10 mg / ml) above MBC were added to bacterial cultures, while the MT-free DMSO was used as a solvent control group, and the MT was added every 20 minutes. The OD600 of the primary bacterial culture was measured, and the growth curve above the melatonin of the minimum bactericidal concentration was drawn. At the same time, 100 μl of bacterial culture is uniformly coated on the Martin agar plate, and it incubated it at 37 ° C for 24 hours to calculate the number of live bacteria, and the bacterial count is performed.

[0119] See figure 2 with image 3 The results show that 4 mg / ml melatonin takes 10 hours to kill all bacteria, while higher concentrations...

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Abstract

The invention provides an application of melatonin in the preparation of drugs for inhibiting and/or killing bacteria, and relates to the technical field of medicine. The research of the present invention finds that small molecular substance melatonin (melatonin), which has significant antibacterial or bactericidal effects, melatonin destroys the metabolic flux of bacteria by specifically targeting citrate synthase in Gram-negative bacteria , in the in vivo infection model of Pasteurella multocida, melatonin effectively reduced bacterial colonization in the lungs and increased the survival rate of mice, providing effective protection for the prevention and/or treatment of pathogenic bacterial infection diseases , providing a theoretical basis for the development of harmless, non-polluting, safe and effective new drugs.

Description

technical field [0001] The invention relates to the technical field of medicine, in particular to the application of melatonin in the preparation of drugs for inhibiting and / or killing bacteria. Background technique [0002] Among human and animal diseases, pathogenic bacteria cause the widest range of infectious diseases, which seriously threaten public health all over the world. So far, the discovery and application of antibiotics undoubtedly play an important role in the fight against bacterial infections. However, the overuse and misuse of antibiotics in the pharmaceutical industry or animal husbandry has irreversibly led to the emergence and prevalence of antibiotic resistance in humans, animals and ecological environments. Shockingly, the plasmid-mediated horizontal transfer of antibiotic resistance genes among and among species accelerates the rapid dissemination of resistant genetic elements. In addition, residual antibiotics in veterinary drugs, animal products, et...

Claims

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Application Information

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IPC IPC(8): A61K31/4045A61P31/14A61P11/00
CPCA61K31/4045A61P11/00A61P31/14A61P31/04
Inventor 任文凯何芳彭远义
Owner SOUTH CHINA AGRI UNIV
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