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Application of melatonin in the preparation of drugs for inhibiting and/or killing bacteria

A technology of melatonin and bacteria, applied in the field of medicine, can solve the problems of bacteria and bacterial infectious diseases that have not yet been reported, and achieve the effects of improving survival, reducing lesions, and inhibiting colonization

Active Publication Date: 2021-03-16
SOUTH CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

But so far, the study of melatonin inhibiting bacteria and bacterial infectious diseases has not been reported.

Method used

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  • Application of melatonin in the preparation of drugs for inhibiting and/or killing bacteria
  • Application of melatonin in the preparation of drugs for inhibiting and/or killing bacteria
  • Application of melatonin in the preparation of drugs for inhibiting and/or killing bacteria

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0107] The test of embodiment 1 melatonin broad-spectrum antibacterial and / or bactericidal

[0108] 1 mL of fresh bacterial suspension (1 × 10 9 CFU) were added to 100mL containing different concentrations of melatonin (50mg / mL, 25mg / mL, 12.5mg / mL, 6.25mg / mL, 3.125mg / mL, 1.5625mg / mL, 0.78125mg / mL, 0.390625mg / mL ), using DMSO dilution without melatonin (MT) as a control group, at 37°C, 120r / min rotating speed, shaking in the dark for 12 hours, to detect the minimum inhibitory concentration MIC of MT on bacteria. Then, suck 0.1mL of the above-mentioned transparent bacterial solution onto a sterilized specific medium agar plate and spread it evenly, incubate in a 37°C incubator for 24 hours to detect the minimum bactericidal concentration MBC of MT to pathogens, and there is no bacteria in it. The lowest concentration for growth was the MBC of the MT against the pathogen. Each experiment was performed 3 times, with 3 repetitions in each group.

[0109] The MIC and MBC results ...

Embodiment 2

[0114] Embodiment 2 melatonin is to the bacteriostasis activity test of bacterium

[0115] To further verify the bacteriostatic activity of melatonin against Pasteurella multocida, bacterial growth curves were determined with increasing concentrations of melatonin. 1 mL of Pasteurella multocida (1.0×10 9 CFU) was added to 100 mL of fresh sterile Martin Broth medium containing MT concentrations lower than MBC (1 mg / mL, 1.5 mg / mL and 2 mg / mL) with DMSO without melatonin as solvent control group. Then the bacteria were cultured in a constant temperature shaker at 37° C. at 220 rpm, and the OD600 of the bacterial culture was measured every 2 hours using an ultraviolet spectrophotometer to detect the influence of melatonin on the bacterial growth curve. Each experiment was performed three times with 3 repetitions in each group.

[0116] Depend on figure 1 The results showed that melatonin could significantly inhibit the growth of bacteria.

Embodiment 3

[0117] Embodiment 3 melatonin kills bacteria

[0118] 1 mL of Pasteurella multocida in logarithmic growth phase (1×10 9 CFU) was added to 100 mL of fresh sterile Martin broth medium. The bacteria were cultured in a constant temperature shaker at 37° C. and 220 rpm for 8 hours. Subsequently, different concentrations of MT (4mg / mL, 8mg / mL, and 10mg / mL) higher than MBC were added to the bacterial culture, while DMSO without MT was used as a solvent control group, every 20 minutes after adding MT. Measure the OD600 of the bacterial culture once, and draw the growth curve under the action of melatonin higher than the minimum bactericidal concentration. At the same time, 100 μL of the bacterial culture was evenly spread on the Martin agar plate, and incubated at 37° C. for 24 h to calculate the number of viable bacteria and perform bacterial counting.

[0119] see results figure 2 with image 3 , the results showed that 4 mg / mL melatonin required 10 hours to kill all bacteria,...

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Abstract

The invention provides an application of melatonin in the preparation of drugs for inhibiting and / or killing bacteria, and relates to the technical field of medicine. The research of the present invention finds that small molecular substance melatonin (melatonin), which has significant antibacterial or bactericidal effects, melatonin destroys the metabolic flux of bacteria by specifically targeting citrate synthase in Gram-negative bacteria , in the in vivo infection model of Pasteurella multocida, melatonin effectively reduced bacterial colonization in the lungs and increased the survival rate of mice, providing effective protection for the prevention and / or treatment of pathogenic bacterial infection diseases , providing a theoretical basis for the development of harmless, non-polluting, safe and effective new drugs.

Description

technical field [0001] The invention relates to the technical field of medicine, in particular to the application of melatonin in the preparation of drugs for inhibiting and / or killing bacteria. Background technique [0002] Among human and animal diseases, pathogenic bacteria cause the widest range of infectious diseases, which seriously threaten public health all over the world. So far, the discovery and application of antibiotics undoubtedly play an important role in the fight against bacterial infections. However, the overuse and misuse of antibiotics in the pharmaceutical industry or animal husbandry has irreversibly led to the emergence and prevalence of antibiotic resistance in humans, animals and ecological environments. Shockingly, the plasmid-mediated horizontal transfer of antibiotic resistance genes among and among species accelerates the rapid dissemination of resistant genetic elements. In addition, residual antibiotics in veterinary drugs, animal products, et...

Claims

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Application Information

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IPC IPC(8): A61K31/4045A61P31/14A61P11/00
CPCA61K31/4045A61P31/14A61P11/00A61P31/04
Inventor 任文凯何芳彭远义
Owner SOUTH CHINA AGRI UNIV
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