Application of melatonin in the preparation of drugs for inhibiting and/or killing bacteria
A technology of melatonin and bacteria, applied in the field of medicine, can solve the problems of bacteria and bacterial infectious diseases that have not yet been reported, and achieve the effects of improving survival, reducing lesions, and inhibiting colonization
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[0107] Example 1 Test of melatonin broad-spectrum antibacterial and / or sterilization
[0108] Bacteria suspension that grows 1ML of fresh alignment (1 × 10 9 CFUs were added to 100 mL, respectively, containing different concentrations of melatonin (50 mg / ml, 25 mg / ml, 12.5 mg / ml, 6.25 mg / ml, 3.125 ml / ml, 1.5625 mg / ml, 0.78125mg / ml, 0.390625 mg / ml) The DMSO dilution containing no melatonin (MT) was used as the control group, and the detection of Mt on the minimum antibacterial concentration MIC of bacteria was performed at 37 ° C, 120 r / min. Then, 0.1 ml of the above-mentioned transparent bacterial solution was absorbed onto the sterilized specific medium agar plate and the coating was uniform, incubated in a 37 ° C incubator for 24 hours, and the minimum bactericidal concentration MBC of the pathogenesis was performed, wherein there were no bacteria The lowest concentration of growth is MT to the MBC of pathogen. Each experiment was carried out 3 times, and 3 r...
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[0114] Example 2 Antibacterial activity test of melatonin on bacteria
[0115] In order to further verify the antibacterial activity of the hyperincardin, the bacterial growth curve is determined in the case of melatonin concentration. Pasteliobacterium in 1 ml of alternatives (1.0 × 10 9 CFU) Add 100 ml of fresh sterile martic broth culture medium, which contains a MT concentration below MBC (1 mg / ml, 1.5 mg / ml and 2 mg / ml), and DMSO containing free melanin as a solvent. Control group. The bacteria were then cultured in a thermostatic closing of 37 ° C, and the OD600 of the primary bacterial culture was determined using an ultraviolet spectrophotometer to detect the effect of melatonin on bacterial growth curve. Each experiment was carried out three times and 3 repetitions per group.
[0116] by figure 1 The results show that melatonin can significantly inhibit bacterial growth.
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[0117] Example 3 Melatonin killing bacteria
[0118] 1 ml of multi-killing Bacillus for several long-term periods (1 × 10 9 CFU) Add 100 ml of fresh sterile martial broth culture medium. The bacteria were cultured in a thermostatic shaker at 37 ° C, 220 rpm. Subsequently, different concentrations of MT (4 mg / ml, 8 mg / ml, and 10 mg / ml) above MBC were added to bacterial cultures, while the MT-free DMSO was used as a solvent control group, and the MT was added every 20 minutes. The OD600 of the primary bacterial culture was measured, and the growth curve above the melatonin of the minimum bactericidal concentration was drawn. At the same time, 100 μl of bacterial culture is uniformly coated on the Martin agar plate, and it incubated it at 37 ° C for 24 hours to calculate the number of live bacteria, and the bacterial count is performed.
[0119] See figure 2 with image 3 The results show that 4 mg / ml melatonin takes 10 hours to kill all bacteria, while higher concentrations...
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