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Skin filler and preparation method thereof

A technology for skin fillers and antibacterial agents, applied in the field of skin fillers and their preparation, can solve the problems of easy formation of granulomas, poor biocompatibility, etc. Effect

Active Publication Date: 2020-08-18
SHENZHEN LANDO BIOMATERIALS
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, studies have shown that such dermal fillers have poor biocompatibility and are prone to granuloma formation

Method used

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  • Skin filler and preparation method thereof
  • Skin filler and preparation method thereof
  • Skin filler and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0071] (1) Placenta pretreatment: method for extracting collagen from placenta tissue: fresh placenta was collected and amniotic membrane was separated from chorion manually. Any remaining blood clots or debris in the amnion and chorion are then removed.

[0072] (2) Extract collagen:

[0073] 1) Homogenize the treated amnion tissue in 0.5M acetic acid, adjust the pH to 2.5 with HCl, and use a 5% pepsin solution by mass percentage according to the volume ratio between the homogenized amnion tissue and the pepsin solution. Digest it with a ratio of 1:40. The digested tissue was then centrifuged; the supernatant was then discarded and the pellet washed with acetone to remove lipids. Then, in the precipitation, according to the ratio of the volume ratio of the precipitation to the pepsin solution of 1:40, add a pepsin solution with a mass percent content of 5%, homogenize, react the mixture at 4 ° C for 12 hours, centrifuge and Discard the supernatant, weigh the precipitate, p...

Embodiment 2

[0079] (1) Placenta pretreatment: method for extracting collagen from placenta tissue: fresh placenta was collected and amniotic membrane was separated from chorion manually. Any remaining blood clots or debris in the amnion and chorion are then removed.

[0080] (2) Extraction of collagen: 1) Homogenize the treated chorion tissue in 0.5M acetic acid, adjust the pH to 2.5 with HCl, and use 5% pepsin solution by mass percentage to follow the homogenization of the chorion after homogenization Digest it with a volume ratio of tissue to pepsin solution of 1:40. The digested tissue was then centrifuged; the supernatant was then discarded and the pellet washed with acetone to remove lipids. Then add pepsin solution with a mass percent content of 5% in the precipitation according to the ratio of the volume of the precipitation to the pepsin solution of 1:40, homogenize, react the mixture at 4 ° C for 24 hours, centrifuge and Discard the supernatant, weigh the pellet, perform the st...

Embodiment 3

[0086] (1) Placenta pretreatment: method for extracting collagen from placenta tissue: fresh placenta was collected and amniotic membrane was separated from chorion manually. Any remaining blood clots or debris in the amnion and chorion are then removed.

[0087] (2) Extract collagen:

[0088] 1) Homogenize the treated amnion tissue in 0.5M acetic acid, adjust the pH to 2.5 with HCl, and use a 5% pepsin solution by mass percentage according to the volume ratio between the homogenized amnion tissue and the pepsin solution. Digest it with a ratio of 1:40. The digested tissue was then centrifuged; the supernatant was then discarded and the pellet washed with acetone to remove lipids. Then, in the precipitation, according to the ratio of the volume of the precipitation to the pepsin solution is 1:40, add a pepsin solution with a mass percent content of 5%, homogenize, react the mixture at 8 ° C for 18 hours, centrifuge and Discard the supernatant, weigh the precipitate, perform...

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Abstract

The invention relates to a skin filler and a preparation method thereof. The skin filler comprises cross-linked collagen and microspheres, wherein the collagen is extracted from human placenta; and the microspheres are selected from at least one of polymethyl methacrylate microspheres, polycaprolactone microspheres, poly-L lactic acid microspheres, polyglycolic acid microspheres and hydroxyapatitemicrospheres. The skin filler has good biocompatibility and stability.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a dermal filler and a preparation method thereof. Background technique [0002] The soft tissue of the human body maintains its structure through proteins such as collagen and elastin, and an extracellular matrix containing mucopolysaccharides. When soft tissue loss occurs due to congenital reasons, external impact, or disease, etc., dermal fillers containing ingredients similar to skin tissue can be injected into a specific site to restore the soft tissue or correct its shape. [0003] Currently commonly used dermal fillers are mainly composed of synthetic hydroxyapatite microspheres, poly-L-lactic acid microspheres, polymethyl methacrylate microspheres and other microspheres. These dermal fillers have a long service life. Good stability. However, studies have shown that such dermal fillers have poor biocompatibility and are prone to granuloma formation. Contents of the inventio...

Claims

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Application Information

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IPC IPC(8): A61L27/48A61L27/46A61L27/54
CPCA61L27/48A61L27/46A61L27/54A61L2300/21A61L2300/216A61L2300/406A61L2300/402A61L2300/41C08L89/00C08L33/12C08L67/04
Inventor 钟梅玲李丽花朱勇军康文亭佘振定谭荣伟
Owner SHENZHEN LANDO BIOMATERIALS
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