Fluorescent probe for rapidly identifying mercury and methyl mercury as well as preparation method and application of fluorescent probe

A fluorescent probe, methylmercury technology, applied in the field of analytical chemistry, can solve problems such as the selective signal of two ions that are difficult to detect, and achieve the effects of improving the detection sensitivity, the equipment is convenient and easy to operate, and the sensitivity is improved.

Active Publication Date: 2020-08-18
BEIJING NORMAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the currently reported probes show the emission of Hg(II) or MeHg always at the same wavelength, making it difficult to simultaneously detect the selective signals of the two ions in vivo.

Method used

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  • Fluorescent probe for rapidly identifying mercury and methyl mercury as well as preparation method and application of fluorescent probe
  • Fluorescent probe for rapidly identifying mercury and methyl mercury as well as preparation method and application of fluorescent probe
  • Fluorescent probe for rapidly identifying mercury and methyl mercury as well as preparation method and application of fluorescent probe

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0052] Example 1: Preparation of small molecule probes

[0053] (1) Dissolve 2-bromo-4'-hydroxyacetophenone (5mmol, 1.075g), 4-dimethylaminobenzaldehyde (5mmol, 0.745g), sodium carbonate (10mmol, 1.060g) in 20ml of ethanol . Then add 5ml of water and stir at 60°C for 6h. After the reactant is cooled, add hydrochloric acid (3ml, 12m) and stir until neutral. The solid was filtered off, and the insoluble matter in the solution was removed after washing with ethanol. The solution was then rotovaped to remove solvent. Product is orange-red solid (1.005g) (55.2%), and wherein, the H spectrum (A), C spectrum (B) of compound BHDP are as figure 2 shown;

[0054] (2) BHDP (1mmol, 0.534g), 4-formylphenylboronic acid (1mmol, 0.149g), potassium carbonate (14mmol, 1.932g), palladium(II) acetate (0.05mmol, 0.011g), triphenylphosphine (0.05 mmol, 0.013 g) was dissolved in a mixture of 1,4-dioxy (20 mL) and water (5 mL). Then, under nitrogen protection, reflux at 80°C for 6h. The solve...

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Abstract

The invention belongs to the field of analytical chemistry, and relates to a fluorescent probe for rapidly identifying mercury and methyl mercury as well as a preparation method and application of thefluorescent probe. The structural formula of the fluorescent probe is shown in the specification. According to the prepared fluorescent probe, under the condition of not adding any other additional material, the detection sensitivity is improved, the additional material is prevented from being added, the consumption of the additional material is reduced, and the error source in detection is reduced; imaging of an object to be detected in living cells and zebra fish bodies is successfully detected in the detection process, the fluorescent probe is suitable for real-time monitoring and distinguishing of the living bodies such as the cells and the zebra fish bodies, and the method cannot be achieved in previous methods. Implementation of imaging plays a great role in promoting deep researchof distinguishing mercury and methyl mercury.

Description

technical field [0001] The invention belongs to the field of analytical chemistry, and relates to a fluorescent probe for quickly identifying mercury and methylmercury, a preparation method and an application thereof. Background technique [0002] At present, most of the researches on Hg(II) and MeHg are indirect reports in hypoxic environment in vitro. Most of the methods for monitoring Hg(II) and MeHg are indirect separation or derivatization of the two ions in vitro. The pretreatment and conventional chromatographic separation are very time-consuming, which makes the in vivo and real-time detection of the two ions impossible. Fluorescence imaging provides a powerful methodological support for studying the physiology and pathology of ions in their natural environment with minimal disturbance to biological systems. Therefore, fluorescence imaging using biocompatible dual-responsive fluorescent probes is an ideal approach to detect and differentiate Hg(II) or MeHg. However...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07C225/22C07C221/00C09K11/06G01N21/64
CPCC07C221/00C07C225/22C09K11/06C09K2211/1007G01N21/6428G01N21/6486
Inventor 那娜付强欧阳津
Owner BEIJING NORMAL UNIVERSITY
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