Serratia marcescens engineering bacterium and application thereof in production of prodigiosin

Abstract

The invention discloses a serratia marcescens engineering bacterium and application thereof in production of prodigiosin, and belongs to the technical field of biology. The invention provides a serratia marcescens engineering bacterium JNB5-1 delta cpxR capable of realizing high yield of prodigiosin. The serratia marcescens engineering bacterium JNB5-1 delta cpxR is obtained by knocking out a genefor coding response regulatory protein CpxR in serratia marcescens JNB5-1. After the serratia marcescens engineering bacterium JNB5-1 delta cpxR is inoculated into a fermentation culture medium and fermented for 96 hours, the yield of prodigiosin in fermentation liquor can be as high as 5.83 g / L and is improved by 41.9% compared with that of wild serratia marcescens JNB5-1.

Description

technical field [0001] The invention relates to a Serratia marcescens engineering bacterium and its application in the production of prodigiosin, belonging to the field of biotechnology. Background technique [0002] Prodigiosin is a kind of natural red pigment containing tripyrrole skeleton structure, which is mainly produced by some microorganisms. It has various biological activities such as anti-cancer, immunosuppression, and anti-insect. extensive attention of researchers. [0003] At present, the methods for producing prodigiosin mainly include chemical synthesis and microbial fermentation. Among them, the chemical synthesis method mainly obtains prodigiosin through tandem conjugate addition and high-temperature dehydrogenation. However, due to complex and difficult pathways and low yields, chemical synthesis is difficult to achieve large-scale industrial production. The principle of microbial fermentation is mainly to obtain prodigiosin through microbial fermentati...

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Problems solved by technology

[0004] However, there are still certain defects in the existing biological methods, among which the low yield is the most important defect hindering the industrialization of microbial fermentation methods

For example, Kim et al. produced prodigiosin by inoculating Hahella chejuensis KCTC 2396 into the 2216 medium of marine bacteria broth for fermentation. However, using this method to ferment for 40 hours, only the concentration of prodigiosin in the fermentation broth Yield up to 28mg / L (specific references: Kim S J, Lee H K, Lee YK, et al. Mutant selection of Hahella chejuensis KCTC 2396 and statistical optimization of medium components for prodigiosin yield-up [J]. ):183-188.); Martha Ingrid Gutiérrez-Román et al fermented Serratia marcescens CFFSUR-B4 by inoculating it into a peanut-based medium to produce prodigiosin, however, using this method for 24h of fermentation, only the fermentation broth The production of prodigiosin and chitinases by tropical Serratia marcescens strains with potential to control plant pathogens [J].World Journal of Microbiology and Biotechnology,2012,28(1):p.145-153.)

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