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Super-resolution imaging method and imaging system based on low-power stimulated emission loss

A technology of stimulated emission loss and super-resolution imaging, which is applied in the field of super-resolution optical microscopy imaging, can solve the problems of high loss laser power and poor quality of super-resolution images, achieve good technical effects, reduce damage, and prolong effective the effect of time

Active Publication Date: 2021-02-26
SHENZHEN UNIV
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Problems solved by technology

[0005] Embodiments of the present invention provide a low-power stimulated emission loss-based super-resolution imaging method and imaging system, aiming to solve the problem of high demand for laser power loss in the stimulated emission loss super-resolution imaging method and super-resolution images under high laser power poor quality problem

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  • Super-resolution imaging method and imaging system based on low-power stimulated emission loss

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Embodiment Construction

[0029] The following will clearly and completely describe the technical solutions in the embodiments of the present invention with reference to the accompanying drawings in the embodiments of the present invention. Obviously, the described embodiments are some of the embodiments of the present invention, but not all of them. Based on the embodiments of the present invention, all other embodiments obtained by persons of ordinary skill in the art without making creative efforts belong to the protection scope of the present invention.

[0030] It should be understood that when used in this specification and the appended claims, the terms "comprising" and "comprises" indicate the presence of described features, integers, steps, operations, elements and / or components, but do not exclude one or Presence or addition of multiple other features, integers, steps, operations, elements, components and / or collections thereof.

[0031] It should also be understood that the terminology used ...

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Abstract

The invention discloses a super-resolution imaging method and imaging system based on low-power stimulated emission loss. By adjusting the position of the corner reflector set in the first optical path, the optical path of the loss laser is extended in the first optical path, and the low-power Gaussian loss laser is converted into a ring-shaped loss laser, which is coincident with the excitation laser and then focused to irradiate the sample. The collected fluorescence lifetime data includes confocal signals and super-resolution signals at the same time. The confocal image and the initial super-resolution image are separated from the fluorescence signal through data processing, and the target super-resolution image with further improved resolution is obtained after analyzing and processing the confocal image and the initial super-resolution image according to the image processing rules. Through the above method, the use of low-power loss laser reduces the damage to biological samples, reduces the photobleaching effect of fluorescent dyes, prolongs the effective time of super-resolution imaging, and can obtain high-resolution images containing fine structural features.

Description

technical field [0001] The invention relates to the technical field of super-resolution optical microscopic imaging, in particular to a super-resolution imaging method and imaging system based on low-power stimulated emission loss. Background technique [0002] Imaging of living cells and tissues is crucial to the research in the field of biomedicine. In addition to correct cell culture conditions and sample preparation methods, advanced imaging methods and imaging systems can maximize the protection of biological characteristics of the observed samples on the basis of maintaining their own biological characteristics. The authenticity and validity of the obtained information. Optical microscopy has the advantages of non-contact, non-injury and specificity, and is an important symbol of the beginning of the development of modern natural science. It can be well applied to the imaging of living cells and tissues. However, the diffraction of light limits the resolution capabili...

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N21/01G01N21/64
CPCG01N21/01G01N21/6402G01N21/6486
Inventor 严伟王璐玮屈军乐王佳林张佳
Owner SHENZHEN UNIV
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