A dual-channel real-time fluorescence quantitative PCR instrument optical path system and detection method

Abstract

An optical path system and detection method of a dual-channel real-time fluorescent quantitative PCR instrument, the optical path system includes a two-color optical excitation module, a microfluidic chip module, a dual-channel fluorescent detection module, and an image processing module; the microfluidic chip module is used for testing samples Amplify and collect fluorescence; the two-color light excitation module is used to make the sample to be tested emit fluorescence, and separate the excitation light emitted by the excitation light source from the fluorescence emitted by the sample to be measured; the dual-channel fluorescence detection module is used to realize different wavelengths of fluorescence. , to adjust the optical path of different wavelengths of fluorescence emitted. The invention adopts dual channels to excite the fluorescence of the sample to be tested, and a single image capture device realizes real-time synchronous imaging of different wavelengths of fluorescence, reducing the cost of the optical path system and the overall instrument; the optical path system is simple in design and compact in structure, reducing the attenuation of optical signal transmission in the optical path system, It is beneficial to the miniaturization and modularization of the overall instrument; avoids interference between excitation light and fluorescence, and improves the receiving signal-to-noise ratio of the image capture device and the detection sensitivity of the instrument.

Description

technical field [0001] The invention relates to the technical field of biomedical detection, in particular to an optical path system and a detection method of a dual-channel real-time fluorescent quantitative PCR instrument. Background technique [0002] The continuous development of life science instruments provides strong guarantee and effective research tools for biomedicine. As a life science instrument widely used in biochemical analysis, clinical diagnosis, disease control screening and other fields, PCR instrument is mainly based on polymerase chain reaction technology to achieve qualitative analysis of the results after a large amount of in vitro amplification of specific DNA fragments in a short period of time. Early PCR instruments can only amplify first and then use the "end point method" for semi-quantitative and qualitative analysis, which is neither real-time nor has disadvantages such as poor detection reproducibility and certain errors. Therefore, real-time ...

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Problems solved by technology

This method needs to select channels and detect them one by one. In this way, it will face the problems of long time required for overall detection, large detector aperture, large space required for optical system structure, and high material cost.

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