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Rhodotorula mucilaginosa immobilized cell and application thereof

A technology of immobilized cells and Rhodotorula japonicum, applied in the immobilized cells of Rhodotorula japonicus and its application fields, can solve the problems of difficult to find parameter combinations, complex selection of immobilization method parameters, etc., to increase the number of reuses and increase the number of repetitions Efficiency of use and broad prospects for industrialization

Pending Publication Date: 2020-08-28
CHENGDU MEDICAL COLLEGE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] So far, there have been no reports on the production of chiral aromatic secondary alcohols by immobilized cells of Rhodotorula glucosa, mainly because the selection of parameters in the immobilization method is complicated and it is difficult to find an excellent combination of parameters.

Method used

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  • Rhodotorula mucilaginosa immobilized cell and application thereof
  • Rhodotorula mucilaginosa immobilized cell and application thereof
  • Rhodotorula mucilaginosa immobilized cell and application thereof

Examples

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experiment example

[0040] EXPERIMENTAL EXAMPLE Immobilization of Rhodotorula gelatinosa

[0041] 1 Materials and methods

[0042] 1.1 Strains

[0043] Rhodotorula mucilaginosa bs5-1.

[0044] 1.2 Reagents

[0045] Sodium Alginate (Sodium-Alginate, SA), Gelatin, Polyvinyl Alcohol (PVA), Carrageenan, Glutaraldehyde, Al 2 (SO 4 ) 3 .

[0046] 2 methods

[0047] 2.1 Preparation of immobilized cells

[0048] 2.1.1 Carrageenan embedding method

[0049] Prepare carrageenan into an aqueous solution of a certain concentration, boil to dissolve, cool to about 45°C, quickly mix with the bacterial suspension and stir evenly (the final concentration of carrageenan is 3%), pour it into a shallow plate, and use 0.3mol / L KCl Soak in the solution, harden at 4°C for 4 hours, and cut into pieces for later use.

[0050] 2.1.2 Gelatin embedding method

[0051] Prepare gelatin into an aqueous solution of a certain concentration, boil and dissolve, then cool, mix and stir evenly with the bacterial suspensio...

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Abstract

The invention provides a rhodotorula mucilaginosa immobilized cell. The cell is prepared by taking sodium alginate as an immobilizing medium and calcium salt as a cross-linking agent and immobilizinga rhodotorula mucilaginosa living cell in gel formed by the immobilizing medium and the cross-linking agent. The invention also provides a preparation method of the immobilized cell. The invention also provides a method for synthesizing aromatic secondary alcohol, and the method is used for catalyzing asymmetric reduction reaction in a water-phase or two-phase system by using the immobilized cellas a catalyst and glucose as an auxiliary substrate. According to the invention, the capacity of rhodotorula mucilaginosa for reducing ACP into (S)-1-PEA can be greatly improved, and the repeated utilization frequency of the rhodotorula mucilaginosa is greatly increased; and compared with free yeast cells, the immobilized cell has very huge advantages and wide industrialization prospect.

Description

technical field [0001] The invention relates to the field of fermentation engineering, in particular to a red yeast cell immobilized and application thereof. Background technique [0002] As important drug intermediates and chiral building blocks, chiral aromatic secondary alcohols play an important role in pharmaceutical synthesis, and yeast is more suitable for the synthesis of chiral aromatic secondary alcohols. However, free cells are not conducive to large-scale production, and the substrate and products have a certain inhibitory effect on yeast cells. Even under the condition of improving the reaction medium, there are still disadvantages of poor cell reusability. [0003] It has been reported that immobilization of yeast cells can slow down the inactivation of the cells, that is, increase the reusability of the cells. After the reaction, the separation of the bacteria and the reaction solution becomes very simple, avoiding cumbersome procedures such as centrifugation ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N11/10C12N11/04C12P7/22C12R1/645
CPCC12N11/10C12N11/04C12P7/22
Inventor 王丹朱冬眉吴明波孙群杨志荣
Owner CHENGDU MEDICAL COLLEGE