Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Gene for increasing diameter of carnation flowers and application

A technology of carnation and flowers, applied in the field of plant genetic engineering, can solve problems such as undiscovered

Active Publication Date: 2020-08-28
YUNNAN UNIV OF TRADITIONAL CHINESE MEDICINE +1
View PDF2 Cites 2 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The flower size of Carnation is an important ornamental trait, and the genes controlling the flower size of Carnation have not been discovered yet

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Gene for increasing diameter of carnation flowers and application
  • Gene for increasing diameter of carnation flowers and application
  • Gene for increasing diameter of carnation flowers and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] Example 1 Carnation OSD La Gene RNAi expression vector construction

[0025] 1) Synthetic OSD La Gene forward and reverse complementary inserts

[0026] exist OSD La Design inserts in non-conserved regions of genes, OSD La The forward insertion fragment is 162 bp, and the sequence is shown in SEQ ID NO.2; OSD La The reverse insertion fragment is 162 bp, the sequence is shown in SEQ ID NO.3, and the DNA fragment is artificially synthesized by Shanghai Jierui Biotechnology Co., Ltd.

[0027] 2) Connection of forward insert fragments

[0028] 30 μL 150 ng / μL PJL10 carrier, the carrier sequence is SEQ ID NO.4, 1 μL KpnI and 1 μL XhoI enzyme, 5 μL common buffer, add water to make up to 50 μL system, put it in a constant temperature incubator for 2-3 hours, and recover the required The purpose strip of about 14 kb is reserved.

[0029] 30 μL 50 ng / μL forward insert fragment to be cut, the sequence is shown in SEQ ID NO.2, 1 μL KpnI and 1 μL XhoI enzyme, 5 μL common buf...

Embodiment 2

[0045] Example 2 Carnation OSD La genetic transformation

[0046] 1) Plant expression vector transformed into Agrobacterium

[0047]Use the freeze-thaw method to transfer the constructed expression vector into Agrobacterium, the specific operation is as follows: Add 2 µg of pJL10-OSDLa-RNAI vector plasmid DNA to 40 microliters of Agrobacterium competent cells that have just been thawed on ice, mix well and place on ice Place for 40 minutes, quick freeze in liquid nitrogen for 5 minutes, 37 ℃ water bath for 3 minutes until completely melted, place on ice for 2 minutes, add to 1ml YEB liquid medium (yeast extract 1g, tryptone 5g, beef extract 5g, magnesium sulfate heptahydrate 0.5g, Add water to 1L, pH 7.0), resume culture at 28 ℃, 200 rpm / min for 3 hours, centrifuge at 5000 rpm / min for 3 minutes, collect the bacteria, and evenly apply to the YEB solid with 50mg / LRif and 50mg / L Kan added in advance On a medium plate (1g of yeast extract, 5g of tryptone, 5g of beef extract, 0.5...

Embodiment 3

[0054] Example 3 Carnation transgenic positive plants OSD La expression and phenotype

[0055] Real-time quantitative fluorescent PCR was used to OSD La Gene expression was analyzed and found OSD La The expression level of the gene decreased in the transgenic positive plants ( figure 1 ), by constructing OSD La The gene RNAi vector obtained 8 transgenic plants and carried out tissue culture and cutting propagation. The average flower diameter of transgenic plants 5-3-B1 is 7.32cm, 3-1-B1 is 6.89cm, 15-2-A3 is 7.09cm, 12-2-C4 is 6.65cm, 12-2-C2 is 7.11cm, 12 -2-C1 averaged 7.33 cm, 1-3-A4 averaged 7.08 cm, and 12-2-B1 averaged 7.23 cm, while the average flower diameter of the control non-transgenic plant 'Noglte' was 5.9 cm. Compared with the wild type, the flower diameter increased, and the flowers became significantly larger ( figure 2 ). The content disclosed in the present invention can be researched OSD La Genes lay the groundwork for regulating the number of pet...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention relates to a gene for increasing the diameter of carnation flowers and application, and belongs to the technical field of plant genetic engineering. The RNA sequence of the gene for increasing the diameter of carnation flowers is shown as SEQ ID NO.1, and the gene is named as OSDLa. A method for increasing the diameter of carnation flowers by adopting the gene specifically comprisesthe following steps: inhibiting the gene expression quantity of the OSDLa gene to obtain a transgenic plant with increased flower diameter. The gene for controlling the diameter of carnation flowers is discovered in the carnation for the first time, the function of the gene for controlling the increase of the diameter of flowers is discovered in plants for the first time, and a candidate gene is provided for other species to clone related genes by utilizing homologous genes. The gene disclosed by the invention can also provide a basis for gene research of increasing the flower diameter of dianthus chinensis and other dicotyledons.

Description

technical field [0001] The invention belongs to the technical field of plant genetic engineering, and specifically relates to a gene for increasing the flower diameter of carnation and its application. Background technique [0002] Flowers are plants with ornamental value. The main parts of flowers for viewing include flower color, flower shape, flower fragrance, flower size, number and number of petals. Flower size is an important ornamental trait, and cultivating new varieties of flowers with large flowers is an important goal of ornamental plant breeding. Some candidate genes related to flower size were found in Arabidopsis, but most of the genes were studied in model plants. It is now found that Arabidopsis HWS and CUC1 genes regulate cell number and petal size, and affect cell proliferation (Gonzalez-Carranza ZH, Zhang X, Peters JL, Boltz V, Szecsi J, Bendahmane M, Roberts JA. (2017) HAWAIIAN SKIRT controls size and floralorgan number by modulating CUC1 and CUC2 expre...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C12N15/29C12N15/82C12N5/10A01H5/02A01H6/30
CPCC07K14/415C12N15/827
Inventor 周旭红桂敏田敏孙瑞芬李莉曹磊叶世隆
Owner YUNNAN UNIV OF TRADITIONAL CHINESE MEDICINE
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com