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Method for preparing sulfated apple pomace polysaccharide through twin-screw extrusion

A technology of twin-screw extrusion and apple pomace polysaccharide, which is applied in the field of food processing, can solve the problems of complex operating conditions, difficult control, and pungent smell of chlorosulfonic acid, and achieve low human toxicity, short residence time, and shortened esterification the effect of time

Inactive Publication Date: 2020-09-01
SHAANXI UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, chlorosulfonic acid-pyridine method is widely used in the sulfation of polysaccharides. The operating conditions of this method are complicated, difficult to control, and chlorosulfonic acid has a pungent smell.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] Example 1

[0023] A method for preparing sulfated apple pomace polysaccharides by twin-screw extrusion, comprising the following steps:

[0024] (1) Weigh 1 part of apple pomace, add 0.3 part of distilled water, stir evenly and let it stand for 0.5h to make the apple pomace fully absorb water; adjust the twin-screw speed to 1200r / min for extrusion modification, and the extruded apple pomace Drying in an oven at 40°C, crushing and passing through a 20-mesh sieve to obtain extrusion-modified apple pomace;

[0025] (2) Weigh 1 part of extrusion-modified apple pomace, add 20 parts of distilled water, place it in a water bath at 40°C for 30 minutes, remove the residue by suction filtration, and concentrate under reduced pressure to 1 / 5 of the original volume. Add 5 times the volume of 95% ethanol for alcohol precipitation to precipitate the polysaccharide, let it stand for 8 hours, centrifuge to discard the supernatant, and freeze-dry the precipitate to obtain the apple po...

Embodiment 2

[0028] A method for preparing sulfated apple pomace polysaccharides by twin-screw extrusion, comprising the following steps:

[0029] (1) Weigh 1 part of apple pomace, add 0.4 part of distilled water, stir evenly and let it stand for 1 hour to make the apple pomace fully absorb water; adjust the twin-screw speed to 1500r / min for extrusion modification, and put the squeezed apple pomace After drying in an oven at 55°C, crushing and passing through a 40-mesh sieve, extrusion-modified apple pomace can be obtained;

[0030] (2) Weigh 1 part of extrusion-modified apple pomace, add 25 parts of distilled water, place it in a water bath for 60 min, heat-preserve and extract for 60 minutes, discard the residue by suction filtration, concentrate under reduced pressure to 1 / 4 of the original volume, add 4 times Alcohol precipitation with 95% ethanol by volume to precipitate the polysaccharide, let it stand for 12 hours, centrifuge to discard the supernatant, and freeze-dry the precipitat...

Embodiment 3

[0033] A method for preparing sulfated apple pomace polysaccharides by twin-screw extrusion, comprising the following steps:

[0034] (1) Weigh 1 part of apple pomace, add 0.5 part of distilled water, stir evenly and let it stand for 2 hours to make the apple pomace fully absorb water; adjust the twin-screw speed to 1800r / min for extrusion modification, and put the squeezed apple pomace After drying in an oven at 70°C, crushing and passing through an 80-mesh sieve, extrusion-modified apple pomace can be obtained;

[0035] (2) Weigh 1 part of extrusion-modified apple pomace, add 30 parts of distilled water, place it in a water bath for 90 min, heat-preserve and extract for 90 minutes, discard the residue by suction filtration, concentrate under reduced pressure to 1 / 3 of the original volume, add 3 times Alcohol precipitation with 95% ethanol by volume to precipitate the polysaccharide, let it stand for 16 hours, centrifuge to discard the supernatant, and freeze-dry the precipit...

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PUM

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Abstract

The invention discloses a method for preparing sulfated apple pomace polysaccharide through twin-screw extrusion, and belongs to the technical field of food processing. The method comprises the following steps: (1) carrying out extrusion modification on apple pomace subjected to moisture adjustment by adopting a double-screw extruder, and performing drying, crushing and sieving to obtain modifiedapple pomace; (2) adding distilled water into the modified apple pomace, performing leaching in a water bath, removing filter residues, concentrating under reduced pressure, carrying out alcohol precipitation, standing, centrifuging, removing supernatant, and drying the precipitate to obtain apple pomace polysaccharide; and (3) dispersing the apple pomace polysaccharide in dimethylformamide, adding sulfamic acid under a stirring condition, uniformly mixing, carrying out esterification reaction under an ultrasonic condition, cooling to room temperature, regulating the pH value until the solution is neutral, carrying out alcohol precipitation, dissolving the obtained precipitate in distilled water, performing dialyzing, and drying the obtained product to obtain the sulfated apple pomace polysaccharide. The method has the advantages of high production efficiency, high yield, safety and no toxicity of the product, and high yield of the obtained product.

Description

technical field [0001] The invention belongs to the technical field of food processing, and in particular relates to a method for preparing sulfated apple pomace polysaccharide by twin-screw extrusion. Background technique [0002] Apple pomace is a by-product produced in the processing of apple juice, apple sauce, etc. At present, only a small part of apple pomace is used for animal feed, and most of it is discarded as waste, which not only causes waste of resources, but also pollutes the environment. Apple pomace contains a lot of dietary fiber, and the water-soluble polysaccharide in the dietary fiber has important physiological activity. However, due to its low content and poor activity, it is difficult to become a high-quality product. Its content can be increased by some means, such as physical, chemical, biotechnological treatment, etc. The screw extrusion technology makes the material subjected to high temperature, high pressure and high shear force in the barrel un...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08B37/00
CPCC08B37/00C08B37/0003
Inventor 代春吉高银刘宁任歌
Owner SHAANXI UNIV OF SCI & TECH
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