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Culture dish and moxifloxacin drug sensitivity kit and use method thereof

The technology of moxifloxacin and kit is applied in the field of use method and preparation of drug sensitive paper, which can solve the problems of inaccurate measurement, time-consuming, troublesome operation and the like

Inactive Publication Date: 2020-09-01
河北翰林生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the results of drug susceptibility testing by the paper disk method first need to measure the diameter of the inhibition zone with a vernier caliper or ruler, and then compare it with the standard data to get the correct result. Such a detection method is not only cumbersome to operate, but also inaccurate and time-consuming.

Method used

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  • Culture dish and moxifloxacin drug sensitivity kit and use method thereof
  • Culture dish and moxifloxacin drug sensitivity kit and use method thereof
  • Culture dish and moxifloxacin drug sensitivity kit and use method thereof

Examples

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Effect test

preparation example Construction

[0054] The preparation method of the moxifloxacin drug-sensitive paper sheet described in the present invention is a technical means well known to those skilled in the art.

[0055] In some preferred implementations, the preparation method of the moxifloxacin drug-sensitive disc, the steps at least include:

[0056] S1: Prepare a round filter paper, sterilize, dry, and set aside; S2: Soak the round filter paper in the moxifloxacin drug solution and let it stand for 1-2 hours; S3: Soak the round filter paper in step S2 The filter paper moxifloxacin drug solution is pre-frozen at -85°C to -75°C, then vacuum freeze-dried, and sealed and stored to obtain the product.

[0057] In some embodiments, the moxifloxacin drug solution in the step S2, by weight percentage, raw materials include: 1.5-2.5% buffer, 0.05-0.055% moxifloxacin drug, and the balance solvent.

[0058] In some embodiments, the buffer comprises at least Na 2 HPO 4 12H 2 O, NaH 2 PO 4 2H 2 At least one of O and...

Embodiment 1

[0067] A culture dish, such as figure 1 As shown, it includes a culture plate base 1; the culture plate base 1 is provided with several rings 2, and the rings 2 do not overlap each other; the center of the ring is provided with a dot 3; the circle Several scale marks 4 are arranged inside the ring 2 .

[0068] The diameter of the ring 2 is 40mm.

[0069] The diameter of the dot 3 is 6.3±0.3mm.

[0070] The division value of the scale mark 4 is 1mm.

[0071] The number of the rings 2 is four.

[0072] The diameter of the culture plate base 1 is 9±1 cm.

[0073] A moxifloxacin drug-sensitivity kit comprises moxifloxacin drug-sensitivity paper sheets and the culture plate.

[0074] The method for using the moxifloxacin drug susceptibility kit, the steps include:

[0075] (1) Culture medium: sterilize the culture medium and pour it onto the culture plate with a thickness of 3-5mm, and let it stand until the culture medium is solidified to form a culture plate;

[0076] (2) ...

Embodiment 2

[0091] A culture dish, such as figure 1 As shown, it includes a culture dish base 1; several rings 2 are arranged on the culture dish base 1, and the rings 2 do not overlap each other; the center of the ring is provided with a dot 3; the circle Several scale marks 4 are arranged inside the ring 2 .

[0092] The diameter of the ring 2 is 40mm.

[0093] The diameter of the dot 3 is 6.3±0.3mm.

[0094] The division value of the scale mark 4 is 1mm.

[0095] The number of the rings 2 is four.

[0096] The diameter of the culture plate base 1 is 9±1 cm.

[0097] A moxifloxacin drug-sensitivity kit comprises moxifloxacin drug-sensitivity paper sheets and the culture plate.

[0098] The method for using the moxifloxacin drug susceptibility kit, the steps include:

[0099] (1) Culture medium: sterilize the culture medium and pour it onto the culture plate with a thickness of 3-5mm, and let it stand until the culture medium is solidified to form a culture plate;

[0100] (2) Ino...

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Abstract

The invention relates to the technical field of culture dishes and drug sensitivity detection thereof, and particularly provides a culture dish, which comprises a culture dish substrate 1, wherein a plurality of circular rings 2 are arranged on the culture dish substrate 1, the circular rings 2 are not overlapped with one another; a dot 3 is arranged in the center of each circular ring; and a plurality of scale marks 4 are arranged in each circular ring 2. The invention also provides a moxifloxacin drug sensitivity kit, which comprises moxifloxacin drug sensitivity paper and the culture dish,and can be used for sensitivity tests of streptococcus pneumoniae, haemophilus influenzae, hemophilus parainfluenzae, staphylococcus aureus, and the like on the antibacterial drug namely moxifloxacin.

Description

technical field [0001] The invention relates to the technical field of a culture dish and drug sensitivity detection thereof, in particular to a culture dish, a method for using the culture dish and a method for preparing a drug-sensitive paper sheet. Background technique [0002] Antimicrobial drug susceptibility test kit, used to determine the ability of antimicrobial drugs or other antimicrobial preparations to inhibit bacterial growth in vitro, clinically used for routine drug sensitive drugs, and assisting clinical rational use of antimicrobial drugs; when clinical treatment effect is poor, auxiliary Screen and replace antibacterial drugs; monitor changes in the resistance of common pathogens in hospitals or areas; evaluate the antibacterial spectrum and antibacterial activity of new antibacterial drugs, etc. [0003] The disk diffusion method is the most widely used drug susceptibility testing method. In this method, a filter paper sheet containing quantitative antiba...

Claims

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Application Information

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IPC IPC(8): C12M1/22C12M1/34C12Q1/18C12Q1/06
CPCC12M23/10C12M41/36C12Q1/06C12Q1/18
Inventor 石月超苏学军关珊孔梦瑶李昆沈广阔
Owner 河北翰林生物科技有限公司
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