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Domestication and breeding method of tetrahydrofuran efficient degradation strain

A technology of tetrahydrofuran and bacteria, applied in chemical instruments and methods, methods of using microorganisms, separation methods, etc., can solve problems such as long cycle, and achieve the effect of shortening the experimental period

Pending Publication Date: 2020-09-01
CHONGQING TECH & BUSINESS UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] So far, most of the domestication and breeding of microorganisms in experiments still rely on traditional shake flask experiments, which generally take a long period of 30-40 days, and it takes a certain amount of time to specifically determine the optimal growth conditions and degradation capabilities of the strains.

Method used

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  • Domestication and breeding method of tetrahydrofuran efficient degradation strain
  • Domestication and breeding method of tetrahydrofuran efficient degradation strain
  • Domestication and breeding method of tetrahydrofuran efficient degradation strain

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0048] Sample Collection

[0049] The wastewater and sludge in the aeration tank of a chemical company in Chongqing were collected with sterile sampling bottles, and placed in a constant temperature incubator for overnight cultivation at a temperature of 35°C.

Embodiment 2

[0051] Enrichment and primary screening of specific strains

[0052] Choose ten 100mL Erlenmeyer flasks and fill them with 30mL LB medium, and five 100mL Erlenmeyer flasks with 30mL MM medium respectively, and put them in a high-pressure steamer for 30min. After mixing the collected water samples and sludge, put them into the above-mentioned 5 Erlenmeyer flasks containing LB medium, put them in a shaker with a temperature of 37°C and a rotation speed of 180r / min as the first generation of culture for 10 hours; the first generation of LB Inoculate the culture medium with a volume fraction of 2% into another 5 LB medium, and put it into a shaker under the same conditions for second-generation culture for 5 hours; finally, add the second-generation LB medium with a volume fraction of 10% The inoculum amount was inserted into the sterilized inorganic salt medium with tetrahydrofuran as the only carbon source, placed in a shaker at a temperature of 32°C and a rotation speed of 130 ...

Embodiment 3

[0054] Enrichment culture of specific bacterial flora after screening

[0055] Select 5 Erlenmeyer flasks and fill them with 50mL LB medium respectively, and fill them with a certain amount of polyethylene foam as seeds, and then sterilize them. Wait until OD600 in embodiment 2 nm When it is about 0.8, inoculate the second-generation LB medium at a volume fraction of 2% to the LB medium containing polyethylene foam as seeds, and then put it into a shaking table with a temperature of 37°C and a rotation speed of 180r / min. During the culture period, measure OD600 every 2 hours nm , Strictly control the OD600 of the fermentation broth of the strain nm =1.2(±0.02).

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Abstract

The invention discloses a domestication and breeding method for a tetrahydrofuran efficient degradation strain. The method comprises the following steps: collecting a sample, enriching and primarily screening a specific strain, enriching a specific flora, carrying out gradient domestication under different conditions, and re-culturing, separating and purifying the domesticated strain. By utilizingthe method disclosed by the invention, the strains of which the specific component waste gas is removed can be directionally and quickly bred. Besides, the method provided by the invention can determine the growth conditions and degradation characteristics of the strain at the same time, and the separated strain can be better applied to the actual process of biological treatment of organic wastegas, and has important actual application value.

Description

technical field [0001] The invention relates to the technical field of environmental engineering microorganisms, in particular to the breeding process of strains with different characteristics. The technology can be used to determine the optimal growth conditions of specific strains and the maximum removal capacity in actual waste gas treatment processes. Background technique [0002] With the advancement of science and technology, the role of microorganisms in environmental governance has become increasingly important. Compared with other physical and chemical methods, the biological method can achieve a better removal effect on pollutants under milder conditions and at a lower cost, and greatly reduces the risk of secondary pollution. At present, environmental engineering microbial technology has been widely used in the treatment of soil and wastewater. In addition, there are relatively many studies on biological treatment of waste gas in recent years. As the core of this...

Claims

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Application Information

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IPC IPC(8): C12N1/36C12N1/02B01D53/84B01D53/72
CPCC12N1/36C12N1/02B01D53/84B01D53/72Y02A50/20
Inventor 姜岩张哲
Owner CHONGQING TECH & BUSINESS UNIV
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