Technological method for producing 3-hydroxybutanone by utilizing wheat B starch

A technology of hydroxybutanone and process methods, applied in the direction of microorganism-based methods, biochemical equipment and methods, separation/purification of carbonyl compounds, etc., to achieve the effect of reducing raw material costs, realizing high added value utilization, and high added value of products

Active Publication Date: 2020-09-01
SHANDONG FOOD & FERMENT IND RES & DESIGN INST
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  • Abstract
  • Description
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  • Application Information

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Problems solved by technology

[0005] Aiming at the problem of low-value utilization of wheat B starch produced in the current gluten production process, the present invention provides a process for producing 3-hydroxybutanone by using wheat B starch

Method used

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  • Technological method for producing 3-hydroxybutanone by utilizing wheat B starch

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Experimental program
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preparation example Construction

[0040] Preparation of sugar solution: add water to the separated and washed starch to adjust the slurry concentration, adjust the concentration of starch slurry to 10-15Bé, add an appropriate amount of amylase according to the total amount of starch to carry out double-enzyme sugar production (add the amount of enzyme preparation according to the amount recommended by the manufacturer), After the saccharification is completed, centrifuge and collect the supernatant part for 3-hydroxybutanone fermentation.

[0041] Seed medium: glucose 50g / L, yeast extract 10g / L, corn steep liquor 10g / L, sodium chloride 5g / L, prepared with drinking water, pH 7.0.

[0042] 3-Hydroxybutanone Fermentation

[0043] Get the starch sugar solution, adjust it to an appropriate glucose concentration (120-160g / L), and prepare a 3-hydroxybutanone fermentation medium. Control experiments were formulated with oral glucose. After the culture medium is sterilized, strains are added for fermentation.

[004...

Embodiment 1

[0046] The influence of embodiment 1 culture medium on the fermentation of 3-hydroxybutanone

[0047] The experiment used glucose as a control to carry out the 3-hydroxybutanone fermentation experiment to investigate the effect of nitrogen source addition on the fermentation of wheat B starch syrup 3-hydroxybutanone. The results are shown in the following table:

[0048]

[0049] The experimental results showed that the fermentation of 3-hydroxybutanone with B starch syrup as raw material did not require the addition of yeast extract and corn steep liquor, and the yield and conversion rate of 3-hydroxybutanone were significantly higher than those of glucose.

Embodiment 2B

[0050] Embodiment 2 takes B starch syrup as raw material 50L fermentation

[0051] Fresh wheat B starch slurry is used to prepare starch sugar by conventional double-enzyme sugar production process. After saccharification, centrifuge to remove solid residue. The residue can be washed with an equal volume of water, centrifuged, combined with centrifuged supernatant, adjust the appropriate glucose concentration, and add to fermentation In jars, sterilized. Another 2g / L urea was added (sterilized separately, added before inoculation). Inoculate according to the inoculum amount of 5%, and the initial sugar concentration after inoculation is 138g / L, and the appropriate temperature and relative dissolved oxygen concentration are controlled during the fermentation process. Cultivate for 58 hours, measure the glucose concentration and drop below 0.1g / L, and end the fermentation. A total of 35 L of fermentation broth was collected, and the content of 3-hydroxybutanone was determined ...

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Abstract

The invention provides a technological method for producing 3-hydroxybutanone by utilizing wheat B starch, and belongs to the technical field of deep processing of agricultural products. The specificprocess method comprises the following steps: preparing sugar from wheat B starch to obtain a starch sugar solution, preparing a fermentation culture medium by taking the starch sugar solution as a raw material, inoculating a 3-hydroxybutanone production strain into the fermentation culture medium, and carrying out fermentation production to obtain 3-hydroxybutanone. By adopting the process method, not only can the production cost of 3-hydroxybutanone be effectively reduced, but also the additional value of the wheat B starch is effectively increased, so that the process method has a good practical application value.

Description

technical field [0001] The invention belongs to the technical field of deep processing of agricultural products, and in particular relates to a process for producing 3-hydroxybutanone by utilizing wheat B starch. Background technique [0002] The information disclosed in this background section is only intended to increase the understanding of the general background of the present invention, and is not necessarily taken as an acknowledgment or any form of suggestion that the information constitutes the prior art already known to those skilled in the art. [0003] 3-Hydroxybutanone (acetoin), also known as acetoin and acetylmethylmethanol, is a widely used food flavor and important C4 platform compound, and has a wide range of applications in food, medicine, chemical industry, tobacco and other fields. At present, 3-hydroxybutanone products on the market are mainly produced by chemical synthesis, and the price is about 100,000-150,000 yuan / ton. The source of raw materials fo...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P7/26C12N1/20C07C49/17C07C45/78C07C45/83C07C45/82C07C45/81C12R1/125
CPCC12P7/26C12N1/20C07C49/17C07C45/78C07C45/83C07C45/82C07C45/81
Inventor 张家祥赵祥颖刘丽萍韩墨刘建军田延军
Owner SHANDONG FOOD & FERMENT IND RES & DESIGN INST
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