Primer probe system for human fungal infection detection, kit and detection method

Abstract

The invention belongs to the field of biological medicines, and particularly relates to a primer probe system for human fungal infection detection, a kit and a detection method. Firstly, the inventiondiscloses the primer probe system capable of simultaneously detecting various pulmonary fungal infections, and the primer probe system comprises a nucleotide sequence group shown as SEQ ID NO: 1-17.The invention also discloses the kit comprising the primer probe system, and a method for fungus gene detection by using the primer probe system or the kit. The kit disclosed by the invention can be used for rapidly and accurately detecting four fungi including pneumocystis jeroveci, mucor, aspergillus and cryptococcus neoformans with ultrahigh sensitivity; and moreover, the change of the specificdrug-resistant gene in the body of the patient can be rapidly monitored in real time according to a quantitative detection result of the target fungus, the condition of the patient can be evaluated in time, and adjuvant therapy suggestions are provided for clinicians.

Description

technical field [0001] The invention belongs to the field of biomedicine, and in particular relates to a primer probe system, a kit and a detection method for detecting human fungal infection. Background technique [0002] Invasive pulmonary fungal infection (IPFI) refers to bronchopulmonary fungal infection not including fungal parasitism and allergies, accounting for the first place in deep fungal infection. Fungi that cause pulmonary mycoses include Aspergillus, Candida, Cryptococcus, Mucormyces, Pneumocystis and the like. Because the clinical manifestations of pulmonary fungal infection are often non-specific, early diagnosis is difficult, and the condition is easily concealed by the primary disease, which easily leads to misdiagnosis and missed diagnosis. The fatality rate of IPFI patients without timely treatment can be as high as 30%-80%. [0003] Common diagnostic methods for pulmonary fungal infection include: pathological examination, microbiological examination,...

AI Technical Summary

Problems solved by technology

However, its application is often limited due to its invasiveness, and it takes a long time (3-7 days), which easily delays diagnosis and treatment

[0004] At present, there are few direct identification methods or kits for pulmonary fungal infection cases on the market, and most of the existing kits are based on the strains obtained from blood culture, and then further identified by PCR, mass spectrometry and other methods, the steps are cumbersome

Moreover, the detection sensitivity of the methods for direct identification of alveolar lavage fluid samples from patients with fungal infections cannot meet the requirements of clinical levels, and pre-culture of alveolar lavage fluid samples is required.

Although there are kits for the detection of specific fungal infections in foreign countries, most of them are based on the qRT-PCR platform, and the detection of bacteria is single, and it is impossible to detect multiple fungi at the same time

Images