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SNP molecular marker associated with hemi-cellulose content of raw ramie and application thereof

A hemicellulose and molecular marker technology, which is applied in the field of plant genetics and breeding, can solve the problems of heavy workload, time-consuming and laborious determination, and easy to be affected by the environment, so as to achieve the effect of improving efficiency and speeding up the breeding process.

Active Publication Date: 2020-09-01
INST OF BAST FIBER CROPS CHINESE ACADEMY OF AGRI SCI
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Problems solved by technology

[0003]The hemicellulose content is a specific quality trait of ramie. Studies have measured the differences in the composition and content of hemicellulose in different ramie varieties and different parts. It is time-consuming and labor-intensive, and the workload is heavy. At present, there is no report on the use of molecular marker technology to map the QTL of hemicellulose content and obtain effective molecular markers.
Studies have shown that the hemicellulose content of ramie raw hemp is controlled by multiple genes and is easily affected by the environment. The use of conventional breeding methods to improve the hemicellulose content of ramie raw hemp has been slow.

Method used

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  • SNP molecular marker associated with hemi-cellulose content of raw ramie and application thereof

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Embodiment 1

[0022] 1. Determination of hemicellulose content: 319 ramie core germplasms were planted in the experimental field of Yuanjiang City, Hunan Province in 2017, with a row spacing of 70 cm and a plant spacing of 50 cm, planted in a single row. The fertility of the experimental field is medium to high and uniform, there are no tall buildings and trees around it, the drainage and irrigation are good, and the planting depth is consistent. In 2018, the traits of the association analysis group and the verification group were tested respectively. After the field samples were harvested, they were stripped with a simple manual scraper (Yuanjiang No. 2), and dried to obtain raw hemp for hemicellulose determination. Sampling was carried out according to GB5881-86 "Sampling method for physical and chemical properties of ramie", and the hemicellulose content was determined by microwave method (Lengjuan et al., 2008), with three repetitions for each variety.

[0023] Table 1 Association Anal...

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Abstract

The invention relates to the technical field of plant genetic breeding, in particular to an SNP molecular marker associated with the hemi-cellulose content of raw ramie and application thereof. The invention provides an SNP molecular marker associated with the hemi-cellulose content of raw ramie. The molecular marker is positioned on chromosomes 1 of ramie. Through identification, significant correlation exists between the marker and ramie hemi-cellulose. The content of TT genotype hemi-cellulose is remarkably higher than that of CC genotype hemi-cellulose, and the content of heterozygous genotype hemi-cellulose is between those of the two genotypes. By identifying the genotype of the SNP site of the ramie variety, the hemi-cellulose level of raw ramie can be predicted. Therefore, the marker can be used for identifying ramie germplasm conforming to the breeding target in the early growth stage of ramie, and has important significance for early prediction and screening of the hemi-cellulose content level of raw ramie. The breeding efficiency is improved, and the breeding process is accelerated.

Description

technical field [0001] The invention relates to the technical field of plant genetics and breeding, in particular to a SNP molecular marker associated with ramie raw hemp hemicellulose content and application thereof. Background technique [0002] Ramie (Boehmerianivea L.) is a characteristic perennial bast fiber crop in my country, which is diploid with 14 pairs of chromosomes. In addition to cellulose, ramie raw hemp also contains colloidal components such as hemicellulose, pectin, lignin, and lipid wax. It needs to go through a degumming process to remove these colloidal components in order to obtain fibers that can be used for textiles. The degumming process of removing gum is the main link that causes environmental pollution in the process of ramie processing, and has become the bottleneck of the development of ramie industry. Hemicellulose accounts for 50% of the total colloid of ramie fiber, and has high stability to acid and alkali, which is the focus and difficulty...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6895C12N15/11
CPCC12Q1/6895C12Q2600/13C12Q2600/156
Inventor 陈平欧文静朱爱国喻春明陈继康陈坤梅高钢王晓飞
Owner INST OF BAST FIBER CROPS CHINESE ACADEMY OF AGRI SCI
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