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Application of 2 (5H)-furanone in inhibition of marine bacterial microbial coating formation in reduction of juvenile mytilus coruscus adhesion

A technology of microbial film and thick-shelled mussels, applied in the field of marine fouling, can solve the problem of no report on the fouling activity of large-scale fouling organisms

Active Publication Date: 2020-09-04
SHANGHAI OCEAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

2(5H)-furanone has been studied as an effective quorum sensing inhibitor, but its attachment activity to macrofouling organisms such as mussels has not been reported

Method used

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  • Application of 2 (5H)-furanone in inhibition of marine bacterial microbial coating formation in reduction of juvenile mytilus coruscus adhesion
  • Application of 2 (5H)-furanone in inhibition of marine bacterial microbial coating formation in reduction of juvenile mytilus coruscus adhesion
  • Application of 2 (5H)-furanone in inhibition of marine bacterial microbial coating formation in reduction of juvenile mytilus coruscus adhesion

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Embodiment

[0042] The method that 2(5H)-furanone of the present embodiment inhibits the formation of marine bacterial microbial film in reducing the attachment of thick-shelled mussel juveniles comprises the steps:

[0043] 1. Culture of Microbial Capsules

[0044] (1). The bacterial strain P.marina ECSMB14103 was isolated from the natural microbial film formed in the East China Sea (Peng, L.H., Liang, X., Guo, X.P., Yoshida, A., Osatomi, K., Yang, J.L., (2018 ).Completegenome of Pseudoalteromonas marina ECSMB14103, a mussel settlement-inducing bacterium isolated from the East China Sea.Mar.Genom.41,46-49.doi:10.1016 / j.margen.2018.04.001), it was inoculated into Zobell 2216E liquid medium (each liter of medium contains 5.0g of peptone, 1.0g of yeast extract, 0.01g of ferric citrate) in a round-bottomed flask, and place the round-bottomed flask in a constant temperature shaking incubator at 27°C for 24h (darkness) , the rotating speed of the shaker is 200rpm, and the initial bacterial st...

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Abstract

The invention provides an application of 2 (5H)-furanone in inhibition of marine bacterial microbial coating formation in reduction of juvenile mytilus coruscus adhesion, and the method for reducing juvenile mytilus coruscus adhesion by 2 (5H)-furanone comprises the following steps: carrying out enlarged culture on bacteria in a liquid culture medium to obtain an initial bacterial stock solution;after centrifuging, discarding the supernatant, mixing with sterilized filtered seawater, and resuspending to obtain a bacterial solution from which the culture medium is washed away; adding the bacterial liquid and 2 (5H)-furanone into a sterile culture dish containing a sterilized slide for bacterial microorganism coating culture, then adding sterilized filtered seawater to wash the slide to obtain a bacterial microorganism coating, transferring the bacterial microorganism coating into the sterile culture dish, and adding mytilus coruscus juvenile mollusk; according to the method, the quorumsensing inhibitor 2 (5H)-furanone is used for interfering with microbial film formation such as EPS biomass and a bacterial metabolism process so that attachment of mytilus coruscus juvenile mollusksis remarkably reduced, and a basis is provided for the field of biological pollution prevention.

Description

technical field [0001] The invention belongs to the technical field of marine fouling, and specifically relates to the application of 2(5H)-furanone to inhibit the formation of marine bacterial microbial film in reducing the adhesion of thick-shelled mussel juveniles, specifically using the quorum sensing inhibitor 2(5H)- Furanone directly inhibits the formation of marine bacteria (Pseudoaltermonas marina ECSMB14103) microbial film and indirectly significantly reduces the attachment of sclerosus mussel spat. Background technique [0002] In marine environments, microbial coatings rapidly form on the surface of various substrates immersed in water. Microbial film is mainly composed of bacteria, diatoms and fungi, etc. Microbial film plays an important role in the attachment and metamorphosis of marine invertebrates such as thick-shelled mussels. Larvae of different marine species respond differently to microbial envelopes. The marine microbial envelope is one of the importa...

Claims

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Application Information

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IPC IPC(8): A01N43/08A01P1/00A01P17/00
CPCA01N43/08
Inventor 李一峰程之扬梁箫朱昕杨金龙
Owner SHANGHAI OCEAN UNIV
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