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Bacterial secretome for use in treatment of skin lesions

A technology for skin damage and secretions, applied in the field of cosmetic or dermatological compositions, which can solve cosmetic problems, unsightly problems, etc.

Active Publication Date: 2020-09-04
PIERRE FABRE DERMO COSMETIQUE CORP
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] Cosmetic issues can also arise from healing when defective or poor healing results in unsightly and often permanent visible marks such as thick scars or keloids

Method used

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  • Bacterial secretome for use in treatment of skin lesions
  • Bacterial secretome for use in treatment of skin lesions
  • Bacterial secretome for use in treatment of skin lesions

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0120] Embodiment 1: the cultivation of bacteria LMB64

[0121] As non-limiting examples, preferred media contain ammonium chloride, magnesium sulfate, and yeast extract. It should also be noted that, as can be seen from application WO2012 / 085182, other similar media may be used and must therefore be considered as forming an integral part of this description. Any modifications by those skilled in the art must also be considered part of the invention.

[0122] An exemplary culture procedure is described below. It should be remembered here that this example is for illustrative purposes only and should in no way be considered restrictive.

[0123] Strain LMB64 was grown in three steps, namely first inoculation, pre-cultivation (or pre-fermentation) in batch mode, and finally cultivation in fed-batch mode (glucose addition).

[0124] Inoculation: Use one tube of WCB LMB64 to inoculate an Erlenmeyer flask containing 1000 mL of sterile medium. Then place the Erlenmeyer flask i...

Embodiment 2

[0137] Example 2: Extraction of the secretome

[0138] The following examples are given by way of illustration of preferred embodiments, but should in no way be considered limiting.

[0139] Typically, the secretome is obtained after centrifugation of the product of the culture step to remove cells, surface proteins, and proteins located in the periplasmic space of the bacteria. After this centrifugation step, Tris-arginine basic buffer was added to the supernatant. The step of filtering the supernatant may also be the last step. Any modifications by those skilled in the art must also be considered part of the invention.

[0140] Centrifugation: The transfer line from the fermenter to the centrifuge is sterilized. The ferment was then separated by continuous centrifugation on a centrifuge. The centrifugation is carried out at 150L / h (±30L / h), and the rotating speed of the drum is 10900±1000rpm. Collect the supernatant in a container with a disposable bag. Measure the w...

Embodiment 3

[0146] Example 3: Effect of Secretome on Proliferation of Normal Human Fibroblasts

[0147] The technique used is the incorporation of the nucleotide, 5-bromo-2'-deoxyuridine (BrdU), a thymidine analog, into the ADN of cells in S phase at 37°C. This technique allows quantification of cell cycle progression characteristic of cells that are proliferative (S or ADN synthesis phase).

[0148] Fibroblasts were seeded into 96-well plates and then incubated for 72 hours in the presence of the compound to be tested, EGF as a positive control, and 0.4%, 0.6% and 1% secretion group. BrdU incorporation was performed over the last 24 hours and quantified using the BrdU ELISA kit (Cat. No. 11647229001, Roche Diagnostics).

[0149] Calculate the % of control according to the following formula:

[0150] (value / mean of control) x 100

[0151] Stimulation in % was calculated according to the following formula:

[0152] Mean % stimulation = (mean(value / mean of control) x 100) - 100

[0153...

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PUM

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Abstract

The present invention relates to a new use of a bacterial secretome in the treatment of skin lesions and more particularly in wound healing. The invention also relates to cosmetic or dermatological compositions comprising the bacterial secretome as active agent.

Description

technical field [0001] The present invention relates to the novel use of bacterial exudome in the field of treatment of skin lesions, especially in the field of wound healing. The invention also relates to cosmetic or dermatological compositions comprising this bacterial exudate group as active agent. Background technique [0002] Wound healing is a complex and dynamic biological process involving the interaction of many local and systemic factors involved in normal tissue repair. Healing occurs in three interdependent phases: hemostasis and inflammation, proliferation and remodeling (General principles of wound healing. Witte MB, Barbul A. Surg Clin North Am. 1997 June; 77(3):509-28). Proliferation involves three clearly observable processes: granulation, shrinkage, and re-epithelialization. [0003] During granulation, cells involved in the repair process were observed to proliferate and migrate towards the wound bed. For example, macrophages, fibroblasts and endothelia...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A61K35/74A61K8/99C12R1/36
CPCA61K8/99A61K35/74C12N1/20A61Q19/00A61Q19/08A61K33/30A61K33/34A61P17/00A61P17/02C12R2001/36C12N1/205A61K2300/00
Inventor N·卡斯泰-里齐M·F·加利亚诺H·赫尔南德斯-皮金
Owner PIERRE FABRE DERMO COSMETIQUE CORP
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