Use of Mouse Models in Assessing Intelligence Levels

A mouse model, intelligence technology, applied in the field of biomedicine, to achieve the effect of high memory ability, high learning ability, improved safety and/or efficacy

Active Publication Date: 2021-01-29
GEMPHARMATECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, no suitable animal resources have been found as research tools, which is a pain point in the development of new drugs for Alzheimer's disease

Method used

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  • Use of Mouse Models in Assessing Intelligence Levels
  • Use of Mouse Models in Assessing Intelligence Levels
  • Use of Mouse Models in Assessing Intelligence Levels

Examples

Experimental program
Comparison scheme
Effect test

Embodiment approach

[0122] 1. A method for constructing a high intelligence level mouse model, said method comprising replacing all or part of the chromosome in the mouse with the chromosome of wild-type mouse origin, thereby making said mouse a high intelligence level mouse .

[0123] 2. The method according to embodiment 1, wherein the wild type mouse is derived from a strain selected from the group consisting of: BLD, CM, DX, FX, HZ, JD, KM, PD, QP, SMX, SY, TW , TZ, YP, YX, ZC, ZZ1 and ZZ2.

[0124] 3. The method according to any one of embodiments 1-2, wherein the mouse is derived from a strain selected from the group consisting of: C57BL / 6J.

[0125] 4. The method of any one of embodiments 1-3, wherein the replacement comprises crossing with the wild type mouse.

[0126] 5. The method according to any one of embodiments 1-4, wherein the replacement comprises means using gene editing.

[0127] 6. The method according to any one of embodiments 1-5, wherein said replacing comprises the step...

Embodiment 1

[0178] Example 1 Mouse preparation and mouse feeding

[0179] Obtain high intelligence level mice (genotype B6 / J-Chr1BLD (jhxiao) / Gpt; or B6 / J-Chr12-ZZ2 (jhxiao) / Gpt) and wild-type mice (gene type) shown in Table 2A and Table 2B type B6 / J), and wild-type mice were used as the control group.

[0180]

[0181]

[0182] Feeding conditions

[0183] The room temperature of the animal is 21-25°C, the humidity is 40%-70%, and the light and dark are alternated for 12 hours.

[0184] Animals were housed in IVC cages or in isolation plastic boxes. Breeding box specifications: length x width x height = 300mm x 190mm x 130 mm, and each box can house up to 5 animals of the same sex.

[0185] The floor of the animal room was disinfected once a day, and 5 different disinfectants were used alternately to avoid microbial resistance. The walls, cages and ceilings were disinfected and wiped at least once a week.

[0186] Animals are free to eat and drink water once a week, and the bott...

Embodiment 2

[0191] Example 2 opening

[0192] experiment procedure:

[0193] 1. Transfer the mouse to the testing room and keep it undisturbed for at least 30 minutes before testing.

[0194] 2. Make sure that the lighting conditions meet the requirements (150-200 lux) and the instrument is operating normally.

[0195] 3. Turn on the computer, turn off the daily lighting in the room, run the video recording software, click the recording button and start timing at the same time, put the mouse into No. 1 at 15s, 30s, 45s, 60s, 75s, 90s, 105s, 120s No. 2, No. 3, No. 4, No. 5, No. 6, No. 7, and No. 8 opening space, ensure that each mouse starts to record data as soon as it is placed, so as to ensure the consistency of the original data; the placement position is one The midpoint of the side edge area faces the direction of the wall, allowing it to freely explore the opening space. After releasing the mice, the experimenter leaves the testing room (out of the sight range of the mice).

[01...

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Abstract

The present application relates to the application of a mouse model in evaluating changes in intelligence level, the mouse model is a mouse model of high intelligence level, and the chromosomes of the mice in the high intelligence level mouse model include at least wild-type mice All or part of chromosome 1 from which the evaluation consists of performing a test selected from the group consisting of the Open Field Test, the Sugar Preference Test, the Water Maze Test, the Spatial Exploration Y-Maze Test, the Active Avoidance Y-Maze Test, and the Fatigue Spin-Rod Test .

Description

technical field [0001] This application relates to the field of biomedicine, in particular to the application of a mouse model in assessing intelligence level. Background technique [0002] Alzheimer's disease (Alzheimer's disease, AD, also known as senile dementia) experimental animal model is an important tool for studying the pathogenesis of the disease, early diagnosis and drug treatment. important factor in clinical failure. Introducing mutant genes related to Alzheimer's disease into animal gene systems to simulate these pathological characteristics and behavioral changes of Alzheimer's disease is the starting point for research on experimental animal models of Alzheimer's disease. [0003] However, the above models all have a late onset, and false positive or false negative results are prone to occur in preclinical drug efficacy testing. This is seriously contrary to the complex phenotype traits of Alzheimer's disease, which are affected by factors such as polygenes...

Claims

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Application Information

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IPC IPC(8): A01K67/02
CPCA01K67/02A01K29/005A01K2227/105A01K2267/03
Inventor 琚存祥赵静王晶晶陈中
Owner GEMPHARMATECH CO LTD
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