Method for preparing artemisia rupestris total flavone by microwave-assisted enzymolysis

A microwave-assisted, total flavonoid technology, applied in the direction of pharmaceutical formulations, medical preparations containing active ingredients, anti-toxins, etc., can solve the problems of long extraction time, large consumption of organic solvents, high power, etc.

Inactive Publication Date: 2020-09-08
伊犁师范大学
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Aiming at the deficiencies of the prior art, the present invention provides a microwave-assisted enzymatic hydrolysis method for preparing total flavonoids of Artemisia annua, which solves the problem that the ultrasonic extraction efficiency is high, but the flavonoid components are destroyed due to too high power or too long extraction time. situation, and the process consumes a large amount of organic solvents, which is not friendly to the environment

Method used

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  • Method for preparing artemisia rupestris total flavone by microwave-assisted enzymolysis
  • Method for preparing artemisia rupestris total flavone by microwave-assisted enzymolysis
  • Method for preparing artemisia rupestris total flavone by microwave-assisted enzymolysis

Examples

Experimental program
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Effect test

Embodiment 1

[0025] S1. Weigh 1.00g Artemisia annua, place it in a conical flask, then add the amount of cellulase in the conical flask, then add 10mL of ethanol solution with a volume fraction of 30%, adjust the pH to 5, and add Under the temperature condition of 30°C, extract for 1.5h. After the reaction is over, put it into a microwave ultrasonic extraction apparatus, set the microwave power to 600W, and the microwave time to 1min;

[0026] S2. According to S1, after the reaction is completed, the solution is rotary evaporated until the ethanol is completely evaporated, and after three extractions with petroleum ether, all the crude extracts of the total flavonoids are transferred to a 50mL volumetric flask, and the volume fraction is 95% ethanol Dilute the solution to volume, shake well and use it as the sample solution to be tested;

[0027] S3, then accurately measure 1mL of the liquid to be tested and place it in a 10mL cuvette, replenish it to 3mL with distilled water, add 0.5mL of...

Embodiment 2

[0030] S1. Weigh 1.00g Artemisia annua, place it in a conical flask, then add the amount of cellulase in the conical flask, then add 40mL of ethanol solution with a volume fraction of 60%, and adjust the pH to 5. Under the temperature condition of 50°C, extract for 1.5h. After the reaction is over, put it into a microwave ultrasonic extraction apparatus, set the microwave power to 600W, and the microwave time to 3min;

[0031] S2. According to S1, after the reaction is completed, the solution is rotary evaporated until the ethanol is completely evaporated, and after three extractions with petroleum ether, all the crude extracts of the total flavonoids are transferred to a 50mL volumetric flask, and the volume fraction is 95% ethanol Dilute the solution to volume, shake well and use it as the sample solution to be tested;

[0032] S3, then accurately measure 1mL of the liquid to be tested and place it in a 10mL cuvette, replenish it to 3mL with distilled water, add 0.5mL of 5% ...

Embodiment 3

[0035] S1. Weigh 1.00g Artemisia annua, put it in a conical flask, then add the amount of cellulase in the conical flask, then add 50mL of ethanol solution with a volume fraction of 70%, adjust the pH to 5, and add Under the temperature condition of 70°C, extract for 1.5h. After the reaction is over, put it into a microwave ultrasonic extractor, set the microwave power to 600W, and the microwave time to 5min;

[0036] S2. According to S1, after the reaction is completed, the solution is rotary evaporated until the ethanol is completely evaporated, and after three extractions with petroleum ether, all the crude extracts of the total flavonoids are transferred to a 50mL volumetric flask, and the volume fraction is 95% ethanol Dilute the solution to volume, shake well and use it as the sample solution to be tested;

[0037] S3, then accurately measure 1mL of the liquid to be tested and place it in a 10mL cuvette, replenish it to 3mL with distilled water, add 0.5mL of 5% NaNO2 sol...

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Abstract

The invention discloses a method for preparing artemisia rupestris total flavone by microwave-assisted enzymolysis. The method specifically comprises the following steps of S1, weighing 1.00 g of artemisia rupestris, placing the weighed artemisia rupestris in a conical flask, then adding cellulase into the conical flask, then adding 10-50 mL of an ethanol solution with the volume fraction of 30-70%, adjusting the pH value to be 5, under the temperature condition that the water bath temperature is 30-70 DEG C, performing extraction for 1.5 h, after a reaction is completed, putting a reaction product into a microwave ultrasonic extractor, and setting the microwave power to be 600 W and the microwave time to be 1-5 min; and S2, according to the step S1, after the reaction is finished, performing rotary evaporation on a solution until ethanol is completely evaporated out. The invention relates to the technical field of flavone extraction. According to the method for preparing the artemisiarupestris total flavone by the microwave-assisted enzymolysis, the Xinjiang artemisia rupestris total flavone can be extracted by adopting the microwave-assisted enzymolysis method, and meanwhile, anoptimal test method and optimal test conditions are explored in different variables, so that the extraction rate of the Xinjiang artemisia rupestris total flavone is increased, and effective resources can be effectively utilized to the maximum extent.

Description

technical field [0001] The invention relates to the technical field of flavonoid extraction, in particular to a method for preparing total flavonoids of Artemisia annua with microwave-assisted enzymatic hydrolysis. Background technique [0002] Xinjiang Yizhi Artemisia is a perennial herb of Asteraceae unique to Xinjiang. It belongs to the Absinthe group of Artemisia genus in Compositae. It is mainly distributed in the northern Xinjiang area. Xinjiang Yizhi Artemisia is one of the commonly used drugs in Uighur medicine. The whole herb is used as medicine. Xinjiang Artemisia argyi is cold in nature and has a bitter taste. It has the effects of clearing heat and detoxifying, invigorating the stomach and eliminating food, calming vomiting, and anti-allergic. It is used to treat poisonous snake bites, urticaria, indigestion, colds, and allergic diseases. The value of development and utilization, as an authentic medicinal material with definite curative effect, Artemisia xinjiang...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/282A61P39/06A61P31/12A61P31/04A61P31/10
CPCA61K36/282A61K2236/19A61K2236/333A61P31/04A61P31/10A61P31/12A61P39/06
Inventor 李雪南光明刘伟王俊龙居尔艾特提拜克
Owner 伊犁师范大学
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