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Nano-drug and preparation method thereof, and application of nano-drug in treatment of pancreatic ductal carcinoma

A nano-medicine and drug technology, applied in the field of biomedicine, to achieve high clinical application value and good biocompatibility

Inactive Publication Date: 2020-09-11
SUN YAT SEN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The latest research shows that UNC0638 has strong toxicity to normal cells

Method used

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  • Nano-drug and preparation method thereof, and application of nano-drug in treatment of pancreatic ductal carcinoma
  • Nano-drug and preparation method thereof, and application of nano-drug in treatment of pancreatic ductal carcinoma
  • Nano-drug and preparation method thereof, and application of nano-drug in treatment of pancreatic ductal carcinoma

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] Preparation of a GSH-responsive drug-loaded UNC06838 nanosystem

[0029] When preparing the drug-loaded Cys-8E nanoparticles, Cys-8E was dissolved in DMSO to form oil phase 1 with a concentration of 20 mg / ml. The drug UNC06838 was dissolved in oil phase 1 at a drug concentration of 9 mg / ml. Stabilizer DSPE-PEG2000 was dissolved in DMSO at a concentration of 4 mg / ml to form oil phase 2.

[0030] Take an appropriate volume of 1 and 2 phase oils and mix them well so that DSPE-PEG2000 is Cys-8E, about 20wt% of the total mass of UNC06838, and add it dropwise to deionized water stirred at a speed of 2000r / m. The volume ratio of the oil phase to the water phase is 1:9. Centrifuge three times using an ultrafiltration tube with a molecular weight cut-off of 100,000 Da to remove

[0031] Free organic solution DMSO, and finally resuspend with PBS. DLS detected that the particle size of the drug-loaded nanoparticles was 100-150nm; TEM images showed that the nanoparticles were u...

Embodiment 2

[0033] Drug Delivery Ability of Polymer Cys-8E

[0034] Load NPs with fluorescent substance coumarin 6 (2.0ug / ml to obtain NP 香豆素 6.

[0035] (1) BxPC-3 (ATCC number CRL-1687, human source, epithelioid adherent growth) PANC-1 cells (ATCC code CRL-1469, human source, epithelioid adherent growth) in the logarithmic growth phase , digested with trypsin, blown into a single cell suspension, counted, inoculated into a 6-well culture plate, 1.5×5 10 per well 5 cells. Cultivate in the incubator for 24 hours. After the cells adhere to the wall, add simple coumarin 6 solution and NP coumarin 6 solution, incubate together at 37°C for 30 minutes, fix with 4% paraformaldehyde and stain with DAPI. nuclear. Fluorescence intensity was recorded by fluorescence microscope and flow cytometer.

[0036] (2) BxPC-3 and PANC-1 cells in the logarithmic growth phase were inoculated on a low-adhesion 24-well plate, and after culturing in a 37°C incubator for 5 days, a single, compact and regular-sh...

Embodiment 3

[0041] NP UNC06838 Comparison of the effects of nanomedicine on the proliferation of pancreatic ductal adenocarcinoma cells.

[0042] Through the cell viability test, detect different concentrations of NP UNC06838 Effects of nanoparticles and UNC06838 free drug on the proliferation of pancreatic ductal adenocarcinoma cells and the formation of tumor spheres by pancreatic ductal adenocarcinoma stem cells.

[0043] (1) The BxPC-3 and PANC-1 cells in the exponential growth phase were digested with trypsin, blown into a single cell suspension, counted, and inoculated into a 96-well culture plate. Cultivate in the incubator for 24 hours, after the cells adhere to the wall, add different concentrations of NP UNC06838 Nanoparticles and UNC06838 free drug were cultured in the incubator for 48 hours, and the cell viability was evaluated according to the instructions of the CellTiter-Glo Luminescent Cell Viability Assay Kit.

[0044] (2) BxPC-3 and PANC-1 cells in the logarithmic gro...

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Abstract

The invention discloses a nano-drug and a preparation method thereof and application of the nano-drug in treatment of pancreatic ductal carcinoma. Specifically, after a nano drug NPUNC0638 is preparedby encapsulating a compound UNC0638 with a nano material (NP) Cys-8E, the nano drug NPUNC0638 can target a tumor part of pancreatic cancer, and has better drug delivery capacity for pancreatic ductaladenocarcinoma cells that do not respond to traditional drugs, and thus the treatment effect of the compound UNC0638 is obviously improved. The nano drug NPUNC0638 can induce pancreatic ductal adenocarcinoma cell apoptosis, and meanwhile, the nano drug NPUNC0638 can prevent UNC0638 from acting on normal cells, so that the toxic and side effects of the nano drug are reduced. The nano-drug NPUNC0638 has the advantages of being capable of targeting pancreatic ductal adenocarcinoma, small in side effect and the like, and has a good application prospect and a wide development space in the field ofclinical treatment of pancreatic ductal adenocarcinoma.

Description

technical field [0001] The invention belongs to the technical field of biomedicine. More specifically, it relates to a nano-medicine, its preparation method and its application in the treatment of pancreatic ductal carcinoma. Background technique [0002] Pancreatic cancer mainly includes adenocarcinoma derived from exocrine cells and neuroendocrine carcinoma derived from islet cells, among which pancreatic ductal adenocarcinoma is one of the most aggressive tumors. Its early clinical symptoms are not typical, diagnosis is difficult, and prognosis is extremely poor. The incidence rate is almost equal to the mortality rate. The 5-year survival rate of patients in the United States is still lower than 7%, while the 5-year survival rate in my country is even lower than 5%. The current treatment methods are mainly surgical resection and adjuvant chemotherapy. However, nearly 80% of the patients had local invasion or even distant organ metastasis at the time of treatment, and l...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K9/51A61K47/34A61K31/517A61P35/00B82Y5/00B82Y40/00
CPCA61K9/5153A61K31/517A61P35/00B82Y5/00B82Y40/00
Inventor 赵蔚吴均殷晓煜
Owner SUN YAT SEN UNIV
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