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Extraction method of phenolic compounds in lepidium latifolium and phenolic compounds extracted by extraction method

A technology of phenolic compounds and extraction methods, which is applied in the field of compound extraction from Lipeacea broadleaf, can solve the problems of extracting phenolic components of Lipega broadleaf, restricting the utilization and research of Lipega broadleaf, and achieves stable and controllable quality, Easy batch preparation and simple preparation process

Active Publication Date: 2020-09-15
CHINA ACAD OF SCI NORTHWEST HIGHLAND BIOLOGY INST
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, there is no definite method to extract the phenolic components in Lepidium latifolia, which seriously limits the utilization and research

Method used

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  • Extraction method of phenolic compounds in lepidium latifolium and phenolic compounds extracted by extraction method
  • Extraction method of phenolic compounds in lepidium latifolium and phenolic compounds extracted by extraction method
  • Extraction method of phenolic compounds in lepidium latifolium and phenolic compounds extracted by extraction method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0041] A kind of extracting method of phenolic acid compounds in unifoliate japonica, comprising the following steps:

[0042] S1. Take 20kg of lentils, dry them at 55°C for 4 days, and then pulverize them to obtain 40-mesh coarse powder;

[0043] S2, extract the coarse powder in step S1 with 95wt% ethanol under ultrasonic conditions of 40KHz and 600W for 3 times, each time for 1h, and mix the obtained extracts, then concentrate under reduced pressure at 65°C and 150hpa for 20min to obtain 1.8kg of extract;

[0044] S3, adding 100kg of water to the medicinal extract in step S2 for dispersion, then adding 100kg of petroleum ether for extraction to obtain 72.6g of extract;

[0045] S4. The extract obtained in step S3 was concentrated under reduced pressure at 65° C. and 150 hpa for 20 minutes to obtain 35 g of extract, which was eluted with a silica gel column eluent after silica gel column chromatography, and the silica gel column eluent was A mixture of dichloromethane, meth...

Embodiment 2

[0050] The difference from Example 1 is that in step S3, adding 100 kg of petroleum ether for extraction is changed to adding 100 kg of ethyl acetate to obtain syringic acid.

[0051] The obtained syringic acid is carried out structural identification, and the identification results are as follows:

[0052] Compound spectral data: syringic acid is a white powder. Molecular formula C 9 h 10 o 5 , 1 H NMR(600MHz,MEOD)δ:7.33(2H,s,H-2,H-6),3.88(6H,s,3,5-OCH 3 ); 13 C-NMR(600MHz,MEOD)δ:168.7(s,C-7),147.4(s,C-3,C-5),140.3(s,C-4),120.7(s,C-1), 106.9(s,C-2,C-6),55.4(s,3,5-OCH 3 ).

Embodiment 3

[0054] The difference from Example 1 is that in step S3, adding 100 kg of petroleum ether for extraction is changed to adding 100 kg of n-butanol to obtain p-hydroxycinnamic acid.

[0055] Spectral data of the compound: p-Hydroxycinnamic acid is white crystal. Molecular formula C 9 h 8 o 3 , 1 H NMR (600MHz, MEOD) δ: 7.60 (1H, d, J = 15.9Hz, H-7), 7.45 (2H, d, J = 8.6Hz, H-3, 5), 6.80 (2H, d, J =8.6Hz,H-2,6),6.28(1H,d,J=15.9Hz,H-8); 13 C-NMR(600MHz,MEOD)δ:169.6(s,C-9),159.8(s,C-1),145.2(s,C-7),129.7(s,C-3,5),125.8( s, C-4), 115.4 (s, C-2, 6), 114.2 (s, C-8).

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Abstract

The invention belongs to the technical field of extraction of compounds in lepidium latifolium, and particularly relates to an extraction method of phenolic compounds in lepidium latifolium and the extracted phenolic compounds. The invention discloses the extraction method of lepidium latifolium phenolic components represented by formula (I). The phenolic compounds are separated from lepidium latifolium, alcohol extraction is carried out on dried whole herb coarse powder of lepidium latifolium, vacuum concentration and extraction are carried out in sequence, an obtained extract is subjected tovacuum concentration, silica gel column chromatography elution is carried out, and MCI column chromatography elution is carried out to obtain the phenolic compounds. The method solves the technical problems that in the prior art, no clear method is provided for extracting the phenolic components in the lepidium latifolium, and utilization and research of the lepidium latifolium are seriously limited.

Description

technical field [0001] The invention belongs to the technical field of extracting compounds from waffles, and in particular relates to a method for extracting phenolic compounds from waffles and the extracted phenolic compounds. Background technique [0002] Lepidium latifolium L. is a plant of the genus Lepidium latifolium in the family Brassicaceae. It is distributed all over the country and can treat visceral congestion, bone disease, rheumatoid arthritis and the resulting edema, high blood pressure and other diseases. Leafy lentils are rich in phenolic compounds. Phenolic components have good anti-oxidation, anti-platelet activity, anti-atherosclerosis, antibacterial, anti-tumor, liver protection and other effects. Leafy lentils are rich in phenolic compounds. [0003] At present, there is no definite method to extract the phenolic components in Lipeperia broadleaf, which severely limits the utilization and research of Lipeperia broadleaf. Contents of the invention ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07C27/26C07C27/34C07C51/42C07C51/48C07C51/47C07C65/21C07C65/03C07C59/48C07C45/78C07C45/79C07C45/80C07C47/58C07C49/84
CPCC07C45/78C07C45/79C07C45/80C07C51/42C07C51/47C07C51/48C07C65/03C07C65/21C07C59/48C07C47/58C07C49/84
Inventor 于瑞涛张玉佩王秀陶燕铎梅丽娟岳会兰吴小可吴楠王卫东杨小兵
Owner CHINA ACAD OF SCI NORTHWEST HIGHLAND BIOLOGY INST
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