Infectious bursal disease virus VP2 protein as well as encoding gene and application thereof

A technology of bursal disease and coding genes, which is applied in the field of genetic engineering in the biotechnology pharmaceutical industry, can solve problems such as strong virulence, damage, and virus recombination, and achieve high economic value, reduce production costs, and huge social benefits. Effect

Pending Publication Date: 2020-09-15
SHANXI AGRI UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, although the moderately virulent live vaccine can resist the attack of the super-virulent strain of IBDV, it will cause certain damage to the bursa of Fabricius in chickens
In addition, the use of live vaccines also has the risk of virus recombination and virulence reverse

Method used

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  • Infectious bursal disease virus VP2 protein as well as encoding gene and application thereof
  • Infectious bursal disease virus VP2 protein as well as encoding gene and application thereof
  • Infectious bursal disease virus VP2 protein as well as encoding gene and application thereof

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Embodiment Construction

[0018] In order to make the purpose, technical solution and advantages of the present invention clearer, the technical solution of the present invention will be described in detail below. Apparently, the described embodiments are only some of the embodiments of the present invention, but not all of them. Based on the embodiments of the present invention, all other implementations obtained by persons of ordinary skill in the art without making creative efforts fall within the protection scope of the present invention.

[0019] 1. Reagents used and their preparation

[0020] (1) LB (Luria-Bertani) liquid medium: Take 10g peptone, 5g yeast powder, 5g NaCl, dissolve in 800mLddH 2 In O, adjust the pH to 7.0 with 10M NaOH, dilute to 1000mL, autoclave (121°C, 1.034×105Pa) for 20min, and store at 4°C.

[0021] (2) LB (Luria-Bertani) solid medium: per 1000mL ddH 2 O add 15g agar powder, add agar powder to LB liquid medium, autoclave, and store at 4°C.

[0022] (3) Stock solution: ...

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Abstract

The invention belongs to the field of genetic engineering in the biotechnological pharmaceutical industry, and particularly relates to infectious bursal disease virus VP2 protein as well as an encoding gene and application thereof. An amino acid sequence of the infectious bursal disease virus VP2 protein is as shown in SEQ ID NO:2. A base sequence of the encoding gene of the infectious bursal disease virus VP2 protein is as shown in SEQ ID NO:1. The infectious bursal disease virus VP2 protein disclosed by the invention well utilizes the advantages of VP2 and Pichia pastoris, and the encoding gene of the infectious bursal disease virus VP2 protein can be efficiently expressed in the Pichia pastoris, so that the production cost is reduced; and the application of the recombinant protein in preparation of infectious bursal disease vaccines has very high economic value and huge social benefit.

Description

technical field [0001] The invention belongs to the field of genetic engineering in the biotechnology pharmaceutical industry, in particular to an infectious bursal disease virus VP2 protein and its coding gene and application. Background technique [0002] Infectious Bursal Disease (IBD) occurs all over the world and causes significant economic losses to the chicken industry. Infectious Bursal Disease Virus (IBDV) is the pathogen that causes IBD. Currently, moderately virulent live vaccines, attenuated vaccines and inactivated vaccines are widely used in the prevention of IBDV. However, although the moderately virulent live vaccine can resist the attack of the super-virulent strain of IBDV, it will cause certain damage to the bursa of Fabricius in chickens. In addition, the use of live vaccines also has the risk of virus recombination and virulence reverse. The risks associated with conventional live attenuated virus vaccines necessitate the development of new vaccines t...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K14/08A61K39/12A61P31/14C12N15/81C12N15/40C12R1/84
CPCC07K14/005A61K39/12A61P31/14C12N15/815C12N2720/10022C12N2720/10034C12N2720/10051C12N2800/22
Inventor 孙娜姜俊兵范阔海李宏全孙耀贵尹伟孙盼盼
Owner SHANXI AGRI UNIV
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