A kind of semiconductor nano biosensor and preparation method thereof

A biosensor and semiconductor technology, applied in the direction of instruments, scientific instruments, material electrochemical variables, etc., can solve the problem of no detection of Staphylococcus aureus, etc., and achieve the effect of improving photocurrent response ability, low background signal and high sensitivity

Active Publication Date: 2022-08-02
DALIAN POLYTECHNIC UNIVERSITY
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  • Claims
  • Application Information

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Problems solved by technology

There are patents and literature reports that detect Staphylococcus aureus with electrochemical immunosensing, optical fiber biosensors, fluorescent sensors, DNA sensors, etc., but there is no use of photoelectrochemical aptamer sensors based on semiconductor nanomaterials to detect Staphylococcus aureus to report

Method used

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  • A kind of semiconductor nano biosensor and preparation method thereof
  • A kind of semiconductor nano biosensor and preparation method thereof
  • A kind of semiconductor nano biosensor and preparation method thereof

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Embodiment Construction

[0033] The present invention will be further described below with reference to the accompanying drawings and specific embodiments. like figure 1 As shown in the figure, the construction process of the working electrode of the semiconductor nano biosensor of the present invention includes sequentially modifying the surface of the ITO electrode with rare earth doped Bi 2 MoO 6 Nanoparticles, Ag 2 S, Au, Aptamer, BSA, FPB (this specific embodiment takes Staphylococcus aureus as an example) to obtain a semiconductor nano-biosensor working electrode, and the specific steps are as follows:

[0034] Step 1. Preparation of Er / Tm / Yb co-doped Bi 2 MoO 6 Nanoparticles

[0035] The already weighed Er 2 O 3 , Tm 2 O 3 , Yb 2 O 3 Dissolve in 50 mL of dilute nitric acid solution (1 mol / L) under the heating condition of 90 ℃ water bath to obtain nitrate solution, when it is completely dissolved and cooled to room temperature, it is recorded as A solution, Er 3+ , Tm 3+ , Yb 3+ T...

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Abstract

The invention discloses a preparation method of a semiconductor nano biosensor. The Er / Tm / Yb-Bi prepared 2 MoO 6 The nanoparticles were ultrasonically treated in water to a uniformly mixed suspension, modified on ITO conductive glass, calcined in a muffle furnace, cooled to room temperature, and then the Ag 2 S was deposited on the BMO‑1 / ITO electrode, and then the gold nanoparticles were modified. Immediately after that, the food-borne pathogenic bacteria aptamer solution was dripped and incubated overnight at 4°C. Continue to drop BSA to block non-specificity, and finally drop food-borne pathogens to successfully build a working electrode. Then a platinum wire electrode was used as the counter electrode, and the saturated calomel electrode was used as the auxiliary electrode. They were placed in PBS solution to construct a three-electrode system, AA was used as the electron donor, and the xenon lamp was used as the light source. biological sensor. The sensor has the advantages of short detection time, high specificity, low cost, and potential portability.

Description

technical field [0001] The invention belongs to the field of food safety detection, and relates to a semiconductor nano biosensor for detecting food-borne pathogenic bacteria and a preparation method thereof. Background technique [0002] Worldwide, with the increasing reliance on processed foods, the number of global food-borne diseases is increasing year by year. According to statistics, hundreds of millions of people around the world suffer from food contamination every year. Among them, the number of patients caused by microbial contamination ranks first, which is the most prominent health and hygiene problem in the world. In recent years, the proportion of food-borne diseases in my country has been on the rise, and about one-sixth of the population suffers from food-borne diseases. Therefore, food poisoning and food-borne diseases caused by food-borne pathogens have become the primary problem of food safety in China. [0003] There are dozens of food-borne pathogens,...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N27/30G01N27/327G01N27/26
CPCG01N27/30G01N27/3278G01N27/26
Inventor 郝洪顺李雪莲朱良良丁超侯红漫张公亮毕景然闫爽
Owner DALIAN POLYTECHNIC UNIVERSITY
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