Probiotic feed additive for improving immunity of aquatic animals

A feed additive, aquatic animal technology, applied in animal feed, animal feed, microorganism-based methods, etc., can solve the problems of poor product effect, slow spore germination, low germination rate, etc., to maintain intestinal flora and eliminate odors. , the effect of improving digestion and absorption rate

Inactive Publication Date: 2020-09-25
QINGDAO BEIBAO OCEAN TECH CO LTD
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Problems solved by technology

In recent years, people have begun to try to use beneficial microorganisms to adjust water quality in aquaculture production, but on the whole, the current probiotic feed additives for aquaculture are still dominated by direct addition of microbial agents. For example, patent CN201810145012.1 discloses a probiotic for aquaculture The preparation method of the feed uses agricultural product processing scraps and/or seafood processing scraps as raw materials, undergoes primary fermentation and ripening and removes miscellaneous bacteria, and then uses biological fermentation technology to carry out secondary fermentation to obtain probiotics rich in three different habits. Bacillus coagulans feed additive for aquaculture containing germination agent; patent CN201811363435.7 discloses a Bacillus coagulans feed additive for aquaculture containing

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  • Probiotic feed additive for improving immunity of aquatic animals

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Comparison scheme
Effect test

Embodiment 1

[0041] A probiotic feed additive for improving the immunity of aquatic animals, the specific preparation process steps include:

[0042] (1) Preparation of fermentation broth: according to the mass percentage, 2% bran, 2% soybean meal, 1% peptone, 1% corn steep liquor, 0.02% potassium dihydrogen phosphate, 0.01% dipotassium hydrogen phosphate, 0.02% magnesium sulfate, calcium chloride 0.01%, the balance ratio is the proportion of water to prepare a liquid fermentation broth, and adjust the pH to 6.5-7.0;

[0043] (2) Aerobic fermentation: Inoculate the seed liquid of Saccharomyces cerevisiae and Bacillus, the inoculum amounts are 2% and 3% respectively, the fermentation temperature is controlled at 28-37°C, and the rotation speed is 150-300rpm; ferment for 24 hours under these fermentation conditions; the spores Bacillus is a mixed bacterial agent of Bacillus subtilis, Bacillus licheniformis and Bacillus pumilus, and its inoculation ratio is 1:2:1;

[0044] (3) Anaerobic ferm...

Embodiment 2

[0052] (1) Preparation of fermentation broth: according to the mass percentage, 2% bran, 2% soybean meal, 1% peptone, 1% corn steep liquor, 0.02% potassium dihydrogen phosphate, 0.01% dipotassium hydrogen phosphate, 0.02% magnesium sulfate, calcium chloride 0.01%, the balance ratio is the proportion of water to prepare a liquid fermentation broth, and adjust the pH to 6.5-7.0;

[0053] (2) Aerobic fermentation: Inoculate the seed liquid of Saccharomyces cerevisiae and Bacillus, the inoculum amounts are 2% and 3% respectively, the fermentation temperature is controlled at 28-37°C, and the rotation speed is 150-300rpm; ferment for 24 hours under these fermentation conditions; the spores Bacillus is a mixed bacterial agent of Bacillus subtilis, Bacillus licheniformis and Bacillus pumilus, and its inoculation ratio is 1:2:1;

[0054] (3) Anaerobic fermentation: After the aerobic fermentation, inoculate Lactobacillus powder, the inoculation amount is 3%, the fermentation temperatur...

Embodiment 3

[0062] A probiotic feed additive for improving the immunity of aquatic animals, the specific preparation process steps include:

[0063] (1) Preparation of fermentation broth: according to the mass percentage, 2% bran, 2% soybean meal, 1% peptone, 1% corn steep liquor, 0.02% potassium dihydrogen phosphate, 0.01% dipotassium hydrogen phosphate, 0.02% magnesium sulfate, calcium chloride 0.01%, the balance ratio is the proportion of water to prepare a liquid fermentation broth, and adjust the pH to 6.5-7.0;

[0064] (2) Aerobic fermentation: Inoculate the seed liquid of Saccharomyces cerevisiae and Bacillus, the inoculum amounts are 2% and 3% respectively, the fermentation temperature is controlled at 28-37°C, and the rotation speed is 150-300rpm; ferment for 24 hours under these fermentation conditions; the spores Bacillus is a mixed bacterial agent of Bacillus subtilis, Bacillus licheniformis and Bacillus pumilus, and its inoculation ratio is 1:2:1;

[0065] (3) Anaerobic ferm...

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Abstract

The invention discloses a probiotic feed additive for improving the immunity of aquatic animals. The formation of the raw materials is as follows in parts by mass: 20-30 parts of probiotic fermented bacterium powder, 30-50 parts of oyster powder, 5-10 parts of cyclodextrin, 2-5 parts of vitamins, 1-2 parts of brown algae polysaccharide, and 0-10 parts of compound enzyme preparation. According to the present invention, aerobic and anaerobic mixed bacterium synergistic fermentation technology is adopted to co-ferment for preparing probiotic fermented feed additives, the immunity enhancement effect of the feed additive is significantly improved through the synergy between fermentation products and microorganisms, and the immunity effect has an important role in inhibiting the infection and growth of pathogenic microorganisms, restoring the balance of intestinal flora, and enhancing the intestinal immune function and physiological function. Experimental results show that the immunity of aquaculture animals such as Penaeus vannamei, can be significantly improved by the product, animal growth performance is improved, the feed coefficient is reduced, the breeding risk is reduced, and thebreeding economic benefit is increased.

Description

technical field [0001] The invention relates to a probiotic feed additive for improving the immunity of aquatic animals, in particular to a probiotic feed additive which can significantly improve the immunity of aquatic animals such as Penaeus vannamei and belongs to the technical field of feed additives. Background technique [0002] In order to meet the ever-increasing demand for aquatic products, solving physiological dysfunction and frequent diseases caused by the continuous expansion of aquaculture scale and shortened growth cycle has become the key problem of aquaculture and the key to the development of the aquafeed industry. In this context, antibiotic feed additives are popular and widely used in the feed industry, which has promoted the development of aquaculture industry to a certain extent, but the problems of high residue, high pollution and drug resistance caused by antibiotics are becoming more and more serious. serious. At present, environmentally friendly f...

Claims

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Application Information

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IPC IPC(8): A23K10/18A23K10/22A23K20/163A23K20/174A23K20/189A23K50/80C12N1/20C12N1/18
CPCA23K10/18A23K10/22A23K20/163A23K20/174A23K20/189A23K50/80C12N1/20C12N1/18C12R2001/10C12N1/205C12R2001/245C12R2001/125C12R2001/225C12R2001/07C12R2001/865C12N1/185A23V2400/125Y02A40/818
Inventor 张凯王浩宇万雪李晓晓
Owner QINGDAO BEIBAO OCEAN TECH CO LTD
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