Ready-to-use vitamin standard substance and preparation method, use method and storage stabilizer thereof

A technology with stable storage and vitamins, which is applied in the field of preparation of vitamin standard products, can solve the problems of easy loss in the weighing process, easy degradation in the storage process, cumbersome preparation process, etc., and achieve the effect of firm structure, improved stability, and not easy to absorb moisture

Pending Publication Date: 2020-09-25
深圳市瑞赛生物技术有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The preparation method of the present invention overcomes technical problems such as the cumbersome preparation process of the existing powdery and liquid vitamin standard substances, easy loss in the weighing process, and easy degradation in the storage process. The vitamin standard product of the present invention contains a series of gradient concentration vitamin standards required for the preparation of the standard curve Substance and storage stabilizer, capable of long-term stable storage under low temperature conditions (≤8°C, 1 year), suitable for vitamin B12, biotin, folic acid, pantothenic acid, vitamin B1, vitamin B2, vitamin B6, niacin and other vitamins

Method used

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  • Ready-to-use vitamin standard substance and preparation method, use method and storage stabilizer thereof
  • Ready-to-use vitamin standard substance and preparation method, use method and storage stabilizer thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] Example 1. Ready-to-Use Vitamin B12 Slat Standards

[0032] The content of vitamin B12 in food is very small, generally in the range of 2-10μg / 100g. In the national standard, ATCC 7830 is used to quantitatively measure the specificity and high sensitivity of vitamin B12. The sensitivity of this method reaches 0.001ng / ml. The vitamin B12 standard is also a lower concentration.

[0033] The vitamin B12 powder standard substance described in this example was purchased from Supelco 47869 with a purity of 99.2%.

[0034] A preparation method of highly stable ready-to-use vitamin B12 slat-type standard substance, which is prepared through the following steps:

[0035] (10) Preparation of vitamin B12 standard stock solution and storage stabilizer;

[0036] (11) Use the storage stabilizer prepared in the above step (10) to fully mix and dilute the vitamin B12 standard stock solution prepared in the above step (10) to obtain the vitamin B12 standard working solution;

[0037]...

Embodiment 2

[0047] Example 2. Ready-to-use Biotin Bottled Standards

[0048] The biotin powder standard substance described in this example was purchased from Supelco 47868 with a purity of 98%.

[0049] A preparation method of high-stability ready-to-use biotin bottled standard substance, which is prepared through the following steps:

[0050] (20) Preparation of biotin standard stock solution and storage stabilizer;

[0051] (21) Use the storage stabilizer prepared in the above step (20) to fully mix and dilute the biotin standard stock solution prepared in the above step (20) to obtain a biotin standard working solution;

[0052] (22) Sterilize the biotin standard working solution and verify the concentration compliance;

[0053] (23) Use vacuum freeze-drying technology to fix the biotin standard mixture with the required concentration in the container, such as figure 2 shown; the required concentration of biotin standard mixture refers to the use of storage stabilizers to dilute t...

example 3

[0062] Example 3. Ready-to-use folic acid centrifuge tube standards

[0063] The folic acid powder standard substance described in this example was purchased from Supelco 47866 with a purity of 98.5%.

[0064] A preparation method of high-stability ready-to-use folic acid standard substance, which is prepared through the following steps:

[0065] (30) Prepare folic acid standard stock solution and prepare storage stabilizer;

[0066] (31) Use the storage stabilizer prepared in the above step (30) to fully mix and dilute the folic acid standard stock solution prepared in the above step (30) to obtain the folic acid standard working solution;

[0067] (32) Sterilize the folic acid standard working solution and verify its concentration compliance;

[0068] (33) Use vacuum freeze-drying technology to fix the folic acid standard mixture of the required concentration in the container; the folic acid standard mixture of the required concentration refers to diluting the folic acid s...

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Abstract

The invention discloses a high-stability ready-to-use vitamin standard substance and a preparation method, a use method and a storage stabilizer thereof. The preparation method comprises the followingsteps of (1) diluting a vitamin standard stock solution by using a storage stabilizer to obtain a vitamin standard working solution,(2) performing sterilization and standard substance concentration conformity verification on the vitamin standard working solution,(3) fixing the vitamin standard mixed solution with the required concentration in a container by adopting a vacuum freeze drying technology, wherein the vitamin standard mixed solution with the required concentration is obtained by diluting the vitamin standard working solution in the step (2) to a series of gradient concentration standard solutions by using a storage stabilizer, and (4) packaging and storing. According to the preparation method of the ready-to-use vitamin standard substance, not only is the long-term storage stability of the low-concentration standard substance improved, but also the prepared vitamin standard substance is rehydrated and dissolved immediately after being used and operated, and the tedious preparation process of a standard solution is omitted.

Description

technical field [0001] The invention belongs to the technical field of vitamin standard product preparation, and in particular relates to a ready-to-use vitamin standard product, a preparation method, a use method and a storage stabilizer. Background technique [0002] Standard A material or substance used to calibrate measuring devices, evaluate methods of measurement, or assign values. Most of the high-purity standard products on the market are small in size and expensive. The powdery standard substance is easily dispersed on the bottle wall and cover. After repeated opening of the bottle, it will easily cause moisture absorption and form agglomerates, while the liquid standard substance will be adsorbed on the bottle. The wall forms a layer of liquid that is difficult to distinguish with the naked eye, resulting in loss in the weighing process and waste. The concentration of the vitamin standard solution is low, and it needs to be weighed with a high-precision balance. T...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N1/28G01N33/00
CPCG01N1/28G01N33/00G01N2001/2893
Inventor 王舒乐齐延林张恒胡云平
Owner 深圳市瑞赛生物技术有限公司
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