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Edible mushroom polysaccharide, and preparation method and application thereof

A technology for edible fungi and mushrooms, which is applied in the field of edible mushroom polysaccharide and its preparation, can solve the problems of poor anti-oxidation and anti-tumor activity, and achieve the effects of improving in vitro anti-oxidation, increasing added value and high efficiency.

Active Publication Date: 2020-09-29
JINAN UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the mushroom edible fungus polysaccharides prepared by the existing methods are often not effective in terms of antioxidant and antitumor activities.

Method used

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  • Edible mushroom polysaccharide, and preparation method and application thereof
  • Edible mushroom polysaccharide, and preparation method and application thereof
  • Edible mushroom polysaccharide, and preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0048] 1. A preparation method of mushroom edible fungus polysaccharide, comprising the steps of:

[0049] (1) Mushroom edible fungus pretreatment: take three portions of fresh Agaricus bisporus, 50g each, samples A, B and C respectively, wash with clean water and dry the surface moisture, take the stipe and cap of the edible fungus and grind them with a pulverizer Crumble into a homogenate, the particle size is about 100 mesh, add 0.1 times the quality of ultrapure water and mix to obtain a homogenate;

[0050] (2) Autolysis and enzymolysis of mushroom edible fungus: Put the homogenate in a water bath, keep sample A at 45°C for 6h, sample B at 45°C for 1.5h, and sample C at 30°C for 6h, and use Stir with a mixer at a stirring speed of 100r / min to obtain the edible fungi of autolyzed mushrooms;

[0051](3) Stop the autolysis of mushroom edible fungus: after the heat preservation is over, heat the autolyzed edible mushroom mushroom obtained in step (2) in a water bath at 100°C...

Embodiment 2

[0099] 1. A preparation method of mushroom edible fungus polysaccharide, comprising the steps of:

[0100] (1) Mushroom edible fungus pretreatment: take three parts of fresh Flammulina velutipes each 50g, which are samples A, B and C respectively, wash with clean water and dry the surface moisture, take the stipe and cap of edible fungus and grind them with a pulverizer Crumble into a homogenate, the particle size is about 50 mesh, add 1 times the quality of ultrapure water and mix to obtain a homogenate;

[0101] (2) Autolysis and enzymolysis of mushroom edible fungus: put the homogenate in a water bath, add 0.5% (w / v) mannanase to sample A, and add 0.5% (w / v) cellulose to sample B Enzyme, add 0.5% (w / v) pectinase to sample C, incubate at 50°C for 2 hours, and stir with a mixer at a stirring speed of 120r / min, to obtain autolytic mushroom edible fungus;

[0102] (3) Stop the autolysis of mushroom edible fungus: After the heat preservation is over, heat the autolyzed mushroom...

Embodiment 3

[0115] A preparation method of mushroom edible fungus polysaccharide, comprising the steps of:

[0116] (1) Mushroom pretreatment: take three parts of fresh shiitake mushrooms, 50g each, wash with water and dry the surface moisture, take the stipe and cap of the edible fungi and crush them into a homogenate with a particle size of about 10 Add 5 times the mass of ultrapure water and mix to obtain a homogenate;

[0117] (2) Autolysis and enzymolysis of mushroom edible fungus: Put the homogenate in a water bath, add 0.5% (w / v) dextranase, keep the A sample at 40°C for 8h, and the B sample at 40°C Insulate for 16 hours, C sample is incubated at 55°C for 8 hours, and stirred with a mixer at a stirring speed of 180r / min;

[0118] (3) Stop the autolysis of edible mushrooms: after the heat preservation, heat in a water bath at 100°C for 10 minutes to inactivate the enzyme activity, stop the autolysis of mushrooms, and obtain a solution of edible mushrooms after autolysis;

[0119] ...

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Abstract

The invention discloses edible mushroom polysaccharide, and a preparation method and application thereof, belonging to the technical field of food biology. The preparation method of the edible mushroom polysaccharide comprises the following steps: (1) pretreating edible mushrooms; (2) carrying out autolysis and enzymolysis on the edible mushrooms; (3) stopping autolysis of the edible mushrooms; and (4) extracting the edible mushroom polysaccharide to obtain crude edible mushroom polysaccharide. According to the preparation method of the edible mushroom polysaccharide, endogenous enzymes of edible mushrooms are fully used for enzymolysis of polysaccharide in the edible mushrooms, and polysaccharide hydrolase is used for degradation, so the in-vitro antioxidant activity and anti-tumor activity of the edible mushroom polysaccharide is improved.

Description

technical field [0001] The invention belongs to the field of food biotechnology, and in particular relates to a mushroom edible fungus polysaccharide and its preparation method and application. Background technique [0002] Edible mushrooms have high economic value. In recent years, the output of edible fungi in my country has been increasing year by year, and has become an important economic crop. At present, there are more than 60 kinds of edible fungi artificially cultivated in my country. The main varieties are shiitake mushroom, bisporus mushroom, enoki mushroom, xiuzhen mushroom and so on. Among them, the output of oyster mushroom, shiitake mushroom and bisporus mushroom is over one million tons. Edible mushrooms are delicious and nutritious, rich in protein, fatty acid, cellulose, mineral elements and other nutrients. In addition, edible fungi also have certain health care functions, such as edible fungus polysaccharides have anti-tumor effects, and tyrosinase in ed...

Claims

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Application Information

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IPC IPC(8): C08B37/00A61P35/00A61P39/06A61K31/715
CPCC08B37/0003C08B37/006A61P35/00A61P39/06A61K31/715
Inventor 彭喜春郭慧
Owner JINAN UNIVERSITY
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