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A kind of pea oligopeptide for lowering blood pressure and its preparation method and application

An oligopeptide and pea technology, which is applied to the preparation methods, applications, chemical instruments and methods of peptides, etc., can solve the problems of few pea oligopeptides and no application reports of pea oligopeptides, so as to reduce blood pressure, reduce adverse effects, The effect of avoiding damage

Active Publication Date: 2021-01-05
YANTAI SHUANGTA FOOD
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0007] At present, there are few studies on pea oligopeptides, and there are no reports on the application of pea oligopeptides in reducing blood pressure. Therefore, it is of great significance to develop the application of pea oligopeptides in reducing blood pressure and functional foods

Method used

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  • A kind of pea oligopeptide for lowering blood pressure and its preparation method and application
  • A kind of pea oligopeptide for lowering blood pressure and its preparation method and application
  • A kind of pea oligopeptide for lowering blood pressure and its preparation method and application

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preparation example Construction

[0032] The preparation method of pea oligopeptide of the present invention comprises the following steps:

[0033] First, Fmoc-Gln(Trt)-OH was reacted with Wang resin as a carrier to generate Fmoc-Gln(Trt)-Resin, and then Fmoc-Pro-OH, Fmoc-Glu(otBu)-OH, Fmoc-Val- OH makes it combine with the previous step reaction product respectively;

[0034] The products of each step in the process are:

[0035] Fmoc-Pro-Gln(Trt)-Resin, Fmoc-Glu(otBu)-Pro-Gln(Trt)-Resin, Fmoc-Val-Glu(otBu)-Pro-Gln(Trt)-Resin;

[0036] Afterwards, the generated Fmoc-Val-Glu(otBu)-Pro-Gln(Trt)-Resin molecule is cleaved to obtain a mixture of Val-Glu-Pro-Gln oligopeptide and other short peptide molecular fragments, and finally purified to obtain a purity greater than 98%. Pure Val-Glu-Pro-Gln pea oligopeptide.

[0037] Among them, Wang resin is Wang resin, Fomc is fluorenyl methaneoxycarbonyl, which plays a protective role in the synthesis of amino acids and can be removed by diethylamine; Trt is trityl, wh...

Embodiment 1

[0040]Through the molecular docking simulation of pea oligopeptide and ACE, the molecular docking is realized by the AutoDock 4.2.6 software package, and the position of the Zn ion in the 1O8A crystal structure is set as the active site, and the peptide VGPG is docked to the active site. The center coordinates of the docking box are set to (36.99, 41.25, 43.45), the number of grid points in each direction of XYZ is set to 80×60×60, the grid point spacing is 0.375 Å, the number of docking times is set to 100, and the rest parameters adopt default values.

[0041] It was found that (see attached figure 2 ), pea oligopeptide forms hydrogen bonds with Ala356 and Arg522 amino acid residues. At the same time, the C-terminal carboxyl group of pea oligopeptide formed a metal coordination bond with Zn ions. Therefore, pea oligopeptide binds to the active site of ACE mainly through hydrogen bonding and coordination with Zn ions, and competitively inhibits ACE, thereby inhibiting the a...

Embodiment 2

[0043] Experimental steps:

[0044] The hypertensive rat model was established by giving rats a high-salt diet containing 5% NaCl and high-sugar drinking water containing 20% ​​fructose. After two weeks of continuous feeding, a non-invasive tail artery sphygmomanometer was used to measure the blood pressure level of the rats to determine whether the model was successfully established. Taking the systolic blood pressure (SBP) greater than 140 mmHg and stable for three consecutive days as the criterion for successful modeling, the rats were divided into normal group (mark Z, normal saline), model group (mark M, normal saline) , control group (label C, felodipine sustained-release tablets 0.5 mg / mL), VGPG high-dose group (label 1, 30 mg / mL), VGPG medium-dose group (label 2, 25 mg / mL), VGPG low-dose group Group (labeled 3, 20 mg / mL), orally administered 5 mL / kg. Blood pressure was measured every 3 days, and the feeding experiment lasted 21 days.

[0045] After the feeding experi...

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Abstract

The invention discloses pea oligopeptide for reducing blood pressure and a preparation method and application of the pea oligopeptide. The molecular formula of the pea oligopeptide is C20H33N5O, the molecular weight of the pea oligopeptide is 471.50, and the pea oligopeptide is named as (S)-5-amino-2-((S)-1-((S)-2-((S)-2-amino-3-methylbutylamine )-4-carboxyl butanoyl )pyrrolidines -2-formylamine )-5-oxopentanedioate acid. The pea oligopeptide has the effects of restraining ACE activity, blocking a renin-angiotensin system, and regulating the diversity and the abundance of intestine floras, andthe proportion of specific floras so as to exert effect of notably reducing blood pressure. The pea oligopeptide can be used for functional foods and tonifying agents.

Description

technical field [0001] The invention belongs to the technical field of oligopeptide extraction and application, and in particular relates to a pea oligopeptide for lowering blood pressure and its preparation method and application. Background technique [0002] In recent years, more than one-third of adults in the world have symptoms of high blood pressure. According to the statistics of "China Cardiovascular Disease Report 2019", the overall prevalence of hypertension in my country is increasing year by year. In addition, high blood pressure can also lead to other complications. More than half of cardiovascular diseases are related to high blood pressure, among which stroke and myocardial infarction are the most common, which seriously threaten the health and life of Chinese residents. [0003] However, simply relying on drugs for the treatment of high blood pressure will lead to various complications and side effects, and nowadays many people only have a tendency to have h...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07K5/103C07K1/06C07K1/04A23L33/18A61K38/07A61P9/12
CPCA23V2002/00A61K38/00A23L33/18A61P9/12C07K5/101A23V2200/30A23V2250/55
Inventor 张树成周泉城杨进洁杨国栋臧庆佳刘长宝武世敏房万才孙钊
Owner YANTAI SHUANGTA FOOD
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