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Biomarkers and their applications for diagnosing the onset of nervonic acid in maple seed oil

A biomarker and nervonic acid technology, applied in biological testing, instruments, measuring devices, etc., can solve problems that are unclear and rarely studied

Active Publication Date: 2020-11-20
BAO FENG BIOTECH (BEIJING) CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

After taking Yuanbao Maple Seed Oil, whether the unique ingredient contained in it-neuric acid can be absorbed and utilized by the body, and at the same time, what structural metabolites will the TG-type nervous acid be converted into in the body, what are its onset markers, these Very important question, so far, there is no clear research to prove it, and even few studies

Method used

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  • Biomarkers and their applications for diagnosing the onset of nervonic acid in maple seed oil
  • Biomarkers and their applications for diagnosing the onset of nervonic acid in maple seed oil
  • Biomarkers and their applications for diagnosing the onset of nervonic acid in maple seed oil

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] 1. Sample collection

[0030] Thirty 5-6 week-old male SD rats were randomly divided into two groups, namely Yuanbao maple seed oil group (NA group) and normal control group (CK group). The rats in each group entered the experiment after adaptive feeding for 1 week. In the Yuanbao Maple Seed Oil group, the dose was calculated according to the drug dose conversion method between humans and rats, and the nervous acid contained in it was 0.03 g / kg / d orally administered once a day, and blood was collected after continuous administration for 1 day. The normal control group was fed routinely, and the sampling method was the same as above.

[0031] 2. Experimental instruments and reagents:

[0032] laboratory apparatus:

[0033] 1. Refrigerated centrifuge: Model D3024R, Scilogex, USA;

[0034] 2. Vortex oscillator: Model MX-S, Scilogex, USA;

[0035] 3. High-resolution mass spectrometer: ESI-QTOF / MS; model: Xevo G2-S Q-TOF; manufacturer: Waters, Manchester, UK;

[0036] 4...

Embodiment 2

[0056] In this embodiment, on the basis of embodiment 1, the blood collected after 3 days of continuous administration is analyzed for test results, and the experimental operation and method are the same as in embodiment 1. The result is as follows:

[0057] 1. Use multivariate statistics to find serum differences

[0058] Orthogonal Partial Least Squares Discriminant Analysis (OPLS-DA) combines Orthogonal Signal Correction (OSC) and Partial Least Squares Regression Analysis (PLS-DA) methods to screen for differential variables by removing irrelevant differences. The result is as Figure 4 , where A is the metabolite with VIP>1 in positive ion mode, B is the metabolite with VIP>1 in negative ion mode, and the VIP value is the variable of the first principal component of Orthogonal Partial Least Squares Discriminant Analysis (OPLS-DA) Importance projection, usually with VIP>1 as a common criterion for metabolomics, as one of the criteria for screening differential metabolites...

Embodiment 3

[0065] In this embodiment, on the basis of embodiment 1, the blood collected after 7 days of continuous administration is analyzed for test results, and the experimental operation and method are the same as in embodiment 1. The result is as follows:

[0066] 1. Use multivariate statistics to find serum differences

[0067] Orthogonal Partial Least Squares Discriminant Analysis (OPLS-DA) combines Orthogonal Signal Correction (OSC) and PLS-DA methods to screen for differential variables by removing irrelevant differences. The result is as Figure 7 is the metabolite with VIP>1 in the positive and negative ion mode, where A is the positive ion mode, B is the negative ion sample, and the VIP value is the variable importance projection of the first principal component of Orthogonal Partial Least Squares Discriminant Analysis (OPLS-DA) , usually taking VIP>1 as a common criterion for metabolomics, as one of the criteria for screening differential metabolites; Figure 8 The score ...

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Abstract

The present invention provides biomarkers for diagnosing the onset of nervonic acid in Ingot maple seed oil, the biomarkers include SM(d17:1 / 24:1) or / and Cer(d18:1 / 24:1(15Z )). The invention also provides the application of the biomarker in the preparation of detection reagents. The biomarkers provided by the present invention for diagnosing the onset of nervonic acid in maple seed oil can be used to judge whether nervonic acid is absorbed and transformed by the body, and provide guidance for whether nervonic acid plays a role after eating maple seed oil .

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a biomarker for diagnosing the onset of nervonic acid and its application. Background technique [0002] Nervonic Acid, also known as Selacholeic Acid, scientific name: cis-15-Tetracosenic Acid (cis-15-Te-tracosenic Acid) molecular formula is C 24 h 46 o 2 , with a molecular weight of 366.6, the pure product is a white needle-like solid at room temperature, and is an n-9 type extra long chain monoene fatty acid. Nervous acid was first discovered in the nervous tissue of mammals, so it is named as nervonic acid. Nervous acid is high in nerve tissue and brain tissue, and is an important component of biofilm. It is usually used as a marker of medulla (white matter) in cerebrosides, and participates in various special physiological functions related to biofilm. Nervous acids are mainly present in human brain proteins, retina, sperm and nerve tissues in the form of glycosp...

Claims

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Application Information

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IPC IPC(8): G01N30/02G01N30/06G01N30/86G01N33/92
CPCG01N30/02G01N30/06G01N30/86G01N33/92G01N2030/062G01N2405/08G01N2800/52G01N2800/285A61P25/28G01N33/6848G01N30/7233
Inventor 陈显扬宋王婷韩佳睿薛腾
Owner BAO FENG BIOTECH (BEIJING) CO LTD
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