Method for mutagenizing soybeans through heavy ion beam C irradiation

A heavy ion beam, irradiation technology, applied in the fields of botanical equipment and methods, applications, plant genetic improvement, etc., can solve the problems of low mutation rate and favorable mutation rate of heavy ion beam irradiation method, and achieve shortened stable speed, The effect of many beneficial types and rich variation materials

Inactive Publication Date: 2020-10-09
NORTHEAST INST OF GEOGRAPHY & AGRIECOLOGY C A S
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The purpose of the present invention is to provide a method for mutagenizing soybeans by heavy ion beam C irradiation in order to solve the technical problem of low mutation rate and favorable mutation rate in the existing heavy ion beam irradiation method

Method used

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Effect test

specific Embodiment approach 1

[0015] Specific embodiment one: the method for the heavy ion beam C radiation mutagenesis soybean of the present embodiment is as follows:

[0016] 1. Use heavy ion beam carbon ions to irradiate the hilum of soybean seeds, the number of soybean seeds irradiated is 450-550, the irradiation dose is 120Gy, the dose rate is 40Gy / min, the irradiation time is 30 minutes, and the sample change time is 15 minutes, get contemporary M 0 seed;

[0017] 2. Using heavy ion beam carbon ions to irradiate contemporary M 0 Seed, Irradiated Contemporary M 0 The number of seeds is 450-550, the irradiation dose is 120Gy, the dose rate is 40Gy / min, the irradiation time is 30 minutes, and the sample change time is 15 minutes. Strains were harvested to obtain M 1 generation;

[0018] 3. In the spring of the second year (early May), the M 1 All individual plants were planted in adult rows, and the unirradiated control varieties were planted at the same time, M 2 The mutant plants of the first ...

specific Embodiment approach 2

[0021] Embodiment 2: This embodiment differs from Embodiment 1 in that the number of soybean seeds irradiated in step 1 is 500. Others are the same as the first embodiment.

specific Embodiment approach 3

[0022] Specific embodiment three: the difference between this embodiment and specific embodiment one or two is that the soybean seeds described in step one have a short growth period, small grains, more than three pods and four pods, moderate yield and strong resistance Variety. Others are the same as those in Embodiment 1 or 2.

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Abstract

The invention discloses a method for mutagenizing soybeans through heavy ion beam C irradiation, relates to a soybean breeding method, and aims to solve the technical problems of low mutation rate andfavorable mutation rate of an existing heavy ion beam irradiation method. The method comprises the following steps of 1, irradiating soybean seed hilum by adopting heavy ion beam carbon ions to obtain current-generation M0 seeds; 2, irradiating the current-generation M0 seeds by adopting the heavy ion beam carbon ions, and performing sowing to obtain a generation M1; 3, in spring of the second year, planting all M1-generation single plants in plant rows, harvesting, numbering and storing all variant single plants, and performing seed testing indoors; 4, in winter of the same year, performingharvesting, numbering and seed testing on strain single plants which are continuously separated from a generation M3; and 5, in spring of the third year, planting the single plants harvested from theM3 to obtain a generation M4, and selecting out a strain with excellent characters and stable inheritance to complete the process. According to the method, the mutation rate can be increased, offspring mutation materials are rich, multiple beneficial types are achieved, and excellent mutation characters such as high protein, lodging resistance, high yield and large grains can be screened out.

Description

technical field [0001] The invention relates to a soybean breeding method. Background technique [0002] The use of X-rays, γ-rays, β-rays, neutrons, lasers, electron beams, ion beams, etc. to induce mutations in plants (seeds, plants, organs) has been widely used. Radiation can produce genetic variation (gene mutation and chromosomal aberration), obtain useful mutants in a short period of time, and directly or indirectly select new varieties that are useful in production. By the end of the 20th century, 56 countries had successfully bred and promoted 2,252 varieties of 162 kinds of plants. Among them, my country’s radiation mutation breeding has successively bred 613 new varieties on more than 40 kinds of plants, accounting for the total number of new varieties of radiation breeding in the world. 27.2%, increasing grain production for the country by 3.3-4 billion kg every year, and creating economic benefits of 3.3 billion yuan. Because radiation breeding has the character...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H1/06
CPCA01H1/06
Inventor 张秋英王雪刘长锴
Owner NORTHEAST INST OF GEOGRAPHY & AGRIECOLOGY C A S
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