A construction method and application of a Yang-deficiency constipation animal model
A technology of animal models and construction methods, which can be applied to pharmaceutical formulations, compound screening/testing, preparations for in vivo experiments, etc., can solve the problems of poor reproducibility and high mortality in animal models of yang-deficiency constipation, and achieve better understanding, Ease of generalization and low mortality
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[0026] Example 1: Establishment of an animal model of yang deficiency type constipation rats induced by acetic acid
[0027] Preparation of reagents: 3% (v / v) acetic acid solution: edible glacial acetic acid solution (CH 3 COOH, Tianyu Food Manufacturing Factory (Korea)) was added into distilled water at room temperature (about 25°C), and stirred with a glass rod to fully mix. When the pH value of the solution was 2.53, stop adding acid.
[0028] 5% (v / v) acetic acid solution: put edible glacial acetic acid solution (CH 3 COOH, Tianyu Food Manufacturing Factory (Korea)) was added into distilled water at room temperature (about 25°C), and stirred with a glass rod to fully mix. When the pH value of the solution was 2.42, stop adding acid.
[0029] Activated carbon ice-water mixture: Take 200 g of activated carbon powder (Fuchen (Tianjin) Chemical Reagent Co., Ltd.) and add it to 1000 mL of ice water to prepare an ice-water mixture containing 20% activated carbon. Stir befo...
experiment example 1
[0082] Experimental Example 1: Establishment of Rat Animal Model of Yang Deficiency Constipation Induced by White Vinegar
[0083] Preparation of reagents: Commercial white vinegar (Shanxi Old Mature Vinegar Factory, batch number: 20140513, total acidity ≥ 6.0 degrees), activated carbon powder (Fuchen (Tianjin) Chemical Reagent Co., Ltd.)
[0084] Experimental animal: same as embodiment 1
[0085] Animal grouping: 30 healthy male SD rats were randomly divided into blank control group (n=10) and white vinegar model group (n=20).
[0086] Modeling method: the first dose of 5mL / rat was given by gavage of Shanxi white vinegar, and the second dose of vinegar was given by gavage of 3 mL / 200g body weight. On the second day after each administration of vinegar, animals were given 16.67 mol / L activated carbon ice water at 0°C by gavage at 5 mL / 200 g body weight. White vinegar and activated charcoal and ice water are used to make models alternately on single and double days. The tota...
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