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A construction method and application of a Yang-deficiency constipation animal model

A technology of animal models and construction methods, which can be applied to pharmaceutical formulations, compound screening/testing, preparations for in vivo experiments, etc., can solve the problems of poor reproducibility and high mortality in animal models of yang-deficiency constipation, and achieve better understanding, Ease of generalization and low mortality

Active Publication Date: 2021-09-28
SHANXI UNIV
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AI Technical Summary

Problems solved by technology

[0007] In order to solve the problems of high mortality rate and poor reproducibility of current yang-deficiency constipation animal models, the present invention provides a construction method and application of yang-deficiency constipation animal models

Method used

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  • A construction method and application of a Yang-deficiency constipation animal model
  • A construction method and application of a Yang-deficiency constipation animal model
  • A construction method and application of a Yang-deficiency constipation animal model

Examples

Experimental program
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Effect test

Embodiment 1

[0026] Example 1: Establishment of an animal model of yang deficiency type constipation rats induced by acetic acid

[0027] Preparation of reagents: 3% (v / v) acetic acid solution: edible glacial acetic acid solution (CH 3 COOH, Tianyu Food Manufacturing Factory (Korea)) was added into distilled water at room temperature (about 25°C), and stirred with a glass rod to fully mix. When the pH value of the solution was 2.53, stop adding acid.

[0028] 5% (v / v) acetic acid solution: put edible glacial acetic acid solution (CH 3 COOH, Tianyu Food Manufacturing Factory (Korea)) was added into distilled water at room temperature (about 25°C), and stirred with a glass rod to fully mix. When the pH value of the solution was 2.42, stop adding acid.

[0029] Activated carbon ice-water mixture: Take 200 g of activated carbon powder (Fuchen (Tianjin) Chemical Reagent Co., Ltd.) and add it to 1000 mL of ice water to prepare an ice-water mixture containing 20% ​​activated carbon. Stir befo...

experiment example 1

[0082] Experimental Example 1: Establishment of Rat Animal Model of Yang Deficiency Constipation Induced by White Vinegar

[0083] Preparation of reagents: Commercial white vinegar (Shanxi Old Mature Vinegar Factory, batch number: 20140513, total acidity ≥ 6.0 degrees), activated carbon powder (Fuchen (Tianjin) Chemical Reagent Co., Ltd.)

[0084] Experimental animal: same as embodiment 1

[0085] Animal grouping: 30 healthy male SD rats were randomly divided into blank control group (n=10) and white vinegar model group (n=20).

[0086] Modeling method: the first dose of 5mL / rat was given by gavage of Shanxi white vinegar, and the second dose of vinegar was given by gavage of 3 mL / 200g body weight. On the second day after each administration of vinegar, animals were given 16.67 mol / L activated carbon ice water at 0°C by gavage at 5 mL / 200 g body weight. White vinegar and activated charcoal and ice water are used to make models alternately on single and double days. The tota...

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Abstract

The invention belongs to the technical field of animal model construction, and provides a method for constructing an animal model of yang deficiency type constipation and its application in order to solve the problems of high mortality and poor reproducibility of the animal model of yang deficiency type constipation. The experimental animals were gavaged with 1-8% v / v acetic acid solution and ice-water mixture containing 5-50% g / v activated carbon for 1-8 weeks. The specific administration method was: the first dose of acetic acid solution was 0.5- 10.0 mL / rat, then 0.5-10 mL / 100g of acetic acid solution, and 0.5-8 hours later, 0.5-10 mL / 100g of ice-water mixture containing activated carbon. The invention provides an animal model of yang-deficiency type constipation with high stability, easy promotion, low mortality, good reproducibility, and economical application, which is used for prescription screening, preclinical research and development of drugs, and functional evaluation for treating yang-deficiency type constipation , mechanism research, etc., as well as the research and development of functional food and health food to provide an experimental basis.

Description

technical field [0001] The invention belongs to the technical field of animal model construction, and in particular relates to a method for constructing an animal model of yang-deficiency type constipation and its application. Background technique [0002] Constipation is a common digestive tract dysfunction disease. Clinical symptoms include dry stool, difficulty or incomplete defecation, and decreased stool weight and frequency. According to epidemiological surveys, in my country, the incidence of constipation is 3% to 17%. With the development of modern society, changes in lifestyle and diet, the incidence of chronic constipation is on the rise, and has the characteristics of universality, recurrence and age correlation. However, the non-fatal nature of constipation often results in neglect of treatment. However, constipation is very harmful, and it can lead to a decline in the patient's quality of life, such as neurasthenia, insomnia, irritability, bad breath, etc. di...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K49/00
CPCA01K67/027A01K2207/20A01K2227/105A01K2267/03A61K49/0008
Inventor 刘晓节赵迪赵思俊李震宇张丽增秦雪梅
Owner SHANXI UNIV
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