Electrochemical immunochromatographic test strip for quantitative detection of novel coronavirus antigen and preparation method thereof

A technology of immunochromatographic test paper and coronavirus, which is applied in the field of biomedical sensing, can solve the problems that limit it to only give qualitative test results, achieve enhanced sensitivity and stability, high-sensitivity quantitative detection, and improve signal-to-noise than the effect

Active Publication Date: 2020-10-16
GUILIN UNIV OF ELECTRONIC TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, most of the relevant lateral flow immunochromatographic test strips use qualitative or semi-quantitative AuNPs and organic dyes as signal markers, although these optical detection-based lateral flow immunochromatographic test strips are still very popular POCT tools nowadays, but Due to the limitation of sensitivity, it can only give qualitative detection results

Method used

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  • Electrochemical immunochromatographic test strip for quantitative detection of novel coronavirus antigen and preparation method thereof
  • Electrochemical immunochromatographic test strip for quantitative detection of novel coronavirus antigen and preparation method thereof
  • Electrochemical immunochromatographic test strip for quantitative detection of novel coronavirus antigen and preparation method thereof

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0053] An electrochemical immunochromatographic test strip for the quantitative detection of novel coronavirus antigens, such as figure 1 As shown, it includes a plastic bottom plate 6, and a paper screen printing electrode 1, a nitrocellulose membrane 4, a binding pad 3, a sample pad 2, and a water-absorbing pad 5 assembled sequentially on the plastic bottom plate 6;

[0054] The paper screen printing electrode 1 is installed in the middle of the plastic bottom plate 6, the nitrocellulose membrane 4 is installed in the middle of the paper screen printing electrode 1, the sample pad 2 is installed on the left side of the nitrocellulose membrane 4, and the two are combined. Pad 3; water-absorbing pad 5 is installed on the right side of nitrocellulose membrane 4; parts of sample pad 2, binding pad 3, nitrocellulose membrane 4 and water-absorbing pad 5 will overlap each other;

[0055] The working part of the paper screen-printed electrode 1 is printed on the hydrophilic area 12,...

Embodiment 2

[0059] A preparation of an immunochromatographic test strip for rapid quantitative detection of novel coronavirus antigens, comprising the following processes:

[0060] 1. Preparation of paper SPEs:

[0061] (1) Take 2.5 mL of SU-8 photoresist, and evenly distribute it on Whatman No. 1 filter paper under the action of a homogenizer. Firstly, the filter paper covered with photoresist was baked on a baking table at 95 °C for 10 min to remove the solvent on the filter paper. Then cover the pre-designed UV mask on the filter paper, stick it tightly, and expose it under the UV exposure lamp for 30 min. The exposed filter paper was baked at 95°C for 3 minutes on a rubber baking table, then soaked in 15 mL of acetone, and then washed three times with 15 mL of isopropanol to completely remove the unpolymerized photoresist on the surface of the filter paper, and finally formed a band Patterned microfluidic paper-based assay devices.

[0062] (2) The working part of the paper SPEs is...

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Abstract

The invention discloses an electrochemical immunochromatographic test strip for rapid quantitative detection of a novel coronavirus antigen and a preparation method thereof. On the basis of a double-antibody sandwich immune reaction, gold platinum core-shell nanoparticles Au@PtNPs have catalytic activity similar to horse radish peroxidase, and the gold platinum core-shell nanoparticles Au@PtNPs are marked on a secondary antibody (antibody2, Ab2) to serve as an electrochemical signal probe Au@PtNPs-Ab2. The integrated electrochemical lateral flow immunochromatography test strip for rapid and quantitative detection of the human serum novel coronavirus antigen is prepared by utilizing the characteristics of rapid reaction and convenient separation of lateral flow immunochromatography. After the immune reaction, a horse radish peroxidase substrate solution is added, and a current signal generated by the Au@PtNPs-Ab2 probe captured in the detection area is measured by adopting a square wavevoltammetry (SWV), so as to realize the quantitative detection of the COVID-19 antigen. The prepared electrochemical lateral flow immunochromatography test paper based on the low-cost and high-performance Au@PtNPs-Ab2 signal probe can provide a new method for rapid, quantitative and instant detection in the aspect of diagnosis of COVID-19.

Description

technical field [0001] The invention belongs to the technical field of biomedical sensing, and in particular relates to a portable electrochemical immunochromatography test strip for quantitative detection of novel coronavirus antigens and a preparation method and application thereof. Background technique [0002] At present, the most commonly used method for clinical diagnosis of COVID-19 is to collect nasopharyngeal secretions or blood samples, isolate and extract the genetic material RNA of the virus from them, and perform real-time fluorescent RT-PCR detection. Although nucleic acid testing is the gold standard for clinical diagnosis of COVID-19, since the implementation of nucleic acid testing requires specialized instruments and standard molecular testing laboratories, the requirements for equipment, venues and operators are extremely high, and the operation is cumbersome and time-consuming. The risk of infection is higher. This also means that nucleic acid testing ca...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/569G01N33/558G01N33/543G01N33/532G01N27/26
CPCG01N33/56983G01N33/558G01N33/532G01N33/54346G01N27/26G01N2469/10G01N2333/165Y02A50/30
Inventor 陈真诚操良丽梁永波赵飞骏肖皓霖朱健铭
Owner GUILIN UNIV OF ELECTRONIC TECH
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