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Kit for determining 5'-ribonuclease

A kit and nucleotidase technology, which is applied in the determination/inspection of microorganisms, measuring devices, instruments, etc., can solve the problems of poor repeatability and anti-interference ability, narrow linear range, and low measurement results.

Pending Publication Date: 2020-10-23
WUHAN KING DIAGNOSTIC TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there are many problems in commercially available kits, such as low detection results for outliers, narrow linear range, poor repeatability and anti-interference ability

Method used

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  • Kit for determining 5'-ribonuclease
  • Kit for determining 5'-ribonuclease
  • Kit for determining 5'-ribonuclease

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] Preparation of reagent R1:

[0030]

[0031] Preparation of reagent R2:

[0032]

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PUM

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Abstract

The invention discloses a kit for determining 5'-ribonuclease. The kit is composed of a reagent R1 and a reagent R2 which are independent of each other. The reagent R1 is prepared from the following components: a buffer solution, a preservative, a protective agent, an anti-interference agent, a surfactant, a reaction enhancer, peroxidase, purine nucleoside phosphorylase, xanthine oxidase, a chromogen substance and water; and the reagent R2 is prepared from the following components: a buffer solution, a protective agent, a reaction enhancer, a preservative, disodium inosinate, 4-aminoantipyrineand water. The kit is high in analysis sensitivity, good in repeatability and high in anti-interference capability, the linearity can reach up to 400U / L, the detection of abnormal specimen results conforms to the actual concentration, and the accuracy of normal specimen test results is not affected.

Description

technical field [0001] The invention relates to the field of in vitro diagnostic reagents, in particular to a 5'-nucleotidase detection kit. Background technique [0002] 5'-nucleotidase is a group of phosphohydrolase with different biochemical properties from alkaline phosphatase, mainly distributed in the heart, liver, brain, muscle, kidney and lung, etc., and distributed in the bile duct and hepatic sinus in the liver and Kupffer cells, the highest content in the liver cytoplasm, metabolized by the liver. The increase of 5'-nucleotidase activity in the blood is mainly seen in hepatobiliary system diseases, liver cancer, hepatitis and liver cirrhosis, especially liver cancer, often accompanied by alpha-fetoprotein, carcinoembryonic antigen and alpha-fucosidase and other liver diseases Simultaneous detection of functional items, comprehensive analysis of liver and gallbladder diseases. [0003] Since the determination of serum 5'-nucleotidase has good specificity and sens...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/48C12Q1/42C12Q1/28C12Q1/26
CPCC12Q1/48C12Q1/42C12Q1/28C12Q1/26G01N2333/9029G01N2333/90235G01N2333/91142Y02A50/30
Inventor 马全新吴艳芳谢小丽
Owner WUHAN KING DIAGNOSTIC TECH CO LTD
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