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Efficient oriental cherry tissue culture and rapid propagation method

A technology of tissue culture rapid propagation and cherry blossoms, which is applied in the field of plant tissue culture, can solve the problems of high contamination rate of explants, small multiplication coefficient, low germination rate, etc., and achieve good cultivation effect, increased reproduction coefficient and shortened reproduction cycle. Effect

Pending Publication Date: 2020-10-30
NINGBO CITY COLLEGE OF VOCATIONAL TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The purpose of the present invention is to provide a method for high-efficiency cherry blossom tissue culture and rapid propagation, and carry out factory production to meet the needs of the market, and solve the problems of high explant contamination rate, low germination rate, and low germination rate of conventional cherry blossom tissue culture and rapid propagation methods on the market The problem of small multiplication coefficient and low rooting rate

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] (a) Acquisition of sterile seedlings: cut the young shoots of annual grass cherry that are healthy and strong, without disease and insect damage; clean the shoots with clean water, remove the leaves, cut the branches containing axillary buds into 3-5cm, and run water first Rinse for 1-2 hours, then soak with 1%-2% washing powder for 5-10m, rinse in running water for 1-2h, then soak in 70% alcohol for 20-30s on a sterile operating table, rinse with sterile water for 3-30 seconds 5 times, then soak in 1% sodium hypochlorite for 10-15m, rinse with sterile water 3-5 times; absorb excess water with absorbent paper, cut the shoots into stems with an axillary bud.

[0021] (b) Induction culture of sterile seedlings: the stem segment with axillary buds is inoculated in the medium, and the medium is MS+6-BA1.0mg / L+NAA 0.2mg / L+sucrose 30g / L+agar powder 8g / L, The pH is 5.8. After the medium is prepared, it is sterilized in a steam autoclave at 121° C. for 20 minutes. The light cu...

Embodiment 2

[0027] (a) Acquisition of aseptic seedlings: cut the young shoots of the annual Pakura urophylla that are healthy and strong, without disease and insect damage; clean the shoots with clear water, remove the leaves, and cut the branches containing axillary buds into 3-5cm, first Rinse with running water for 1-2h, then soak with 1%-2% washing powder for 5-10m, rinse with running water for 1-2h, then soak with 70% alcohol for 20-30s on a sterile operating table, rinse with sterile water for 3 ~5 times, then soaked with 1% sodium hypochlorite for 10-15m, rinsed with sterile water for 3-5 times; blotted excess water with absorbent paper, cut the shoots into stems with an axillary bud.

[0028] (b) Induction culture of sterile seedlings: the stem segment with axillary buds is inoculated in the medium, and the medium is MS+6-BA1.0mg / L+NAA 0.2mg / L+sucrose 30g / L+agar powder 8g / L, The pH is 5.8. After the medium is prepared, it is sterilized in a steam autoclave at 121° C. for 20 minute...

Embodiment 3

[0034] (a) Acquisition of sterile seedlings: cut healthy, strong, annual young shoots of Chinese cherry blossoms without disease and insect damage; clean the shoots with clean water, remove the leaves, cut the branches containing axillary buds into 3-5cm, and run water first Rinse for 1-2 hours, then soak with 1%-2% washing powder for 5-10m, rinse in running water for 1-2h, then soak in 70% alcohol for 20-30s on a sterile operating table, rinse with sterile water for 3-30 seconds 5 times, then soak in 1% sodium hypochlorite for 10-15m, rinse with sterile water 3-5 times; absorb excess water with absorbent paper, cut the shoots into stems with an axillary bud.

[0035](b) Induction culture of sterile seedlings: the stem segment with axillary buds is inoculated in the medium, and the medium is MS+6-BA1.0mg / L+NAA 0.2mg / L+sucrose 30g / L+agar powder 8g / L, The pH is 5.8. After the medium is prepared, it is sterilized in a steam autoclave at 121° C. for 20 minutes. The light culture ...

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Abstract

The invention discloses an efficient oriental cherry tissue culture and rapid propagation method. The efficient oriental cherry tissue culture and rapid propagation method comprises the steps of aseptic seedling acquisition, aseptic seedling induction culture, aseptic seedling subculture, rooting culture, seedling hardening, transplanting and the like; through reasonable matching of multiple stepsand formula selection of a culture medium, the oriental cherry production process is simplified, and the production cost is reduced; and the cherry blossoms bred through the method are high in survival rate and short in growth cycle, and the method is simple in steps, low in culture cost, relatively high in commercial value and practicability and suitable for market popularization.

Description

technical field [0001] The patent of the present invention relates to the technical field of plant tissue culture, and specifically refers to a method for high-efficiency cherry blossom tissue culture and rapid propagation. Background technique [0002] Cherry blossoms are widely loved by the public as ornamental flowers and trees. Cherry blossoms are the collective name of several plants of the genus Sakura in the Rosaceae. In the newly revised name of "Flora of China", they specifically refer to "Tokyo cherry blossoms", also known as "Japanese cherry blossoms". There are quite a variety of cherry blossoms, more than 300 kinds. There are about 150 kinds of wild cherry blossoms in the world, and there are more than 50 kinds in China. Among the wild ancestors of about 40 species of cherry blossoms in the world, 33 species are native to China. Others are varieties derived through horticultural crosses. Cherry blossoms are temperate and subtropical tree species. They like sun...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/008
Inventor 刘峰王志龙何月秋李文林立
Owner NINGBO CITY COLLEGE OF VOCATIONAL TECH
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