Microbial flora compound liquid and petroleum hydrocarbon contaminated soil treatment method

A technology of microbial flora and polluted soil, applied in the direction of microbial-based methods, restoration of polluted soil, biochemical equipment and methods, etc., can solve the problems of vegetation destruction, water pollution, soil enzyme activity reduction, etc., and achieve vigorous metabolism , Improve the degradation efficiency, the effect of strong degradation ability

Pending Publication Date: 2020-10-30
上海傲江生态环境科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Petroleum hydrocarbon pollution will seriously affect the use of land, causing changes in soil structure and properties, microbial community changes, soil enzyme ac

Method used

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  • Microbial flora compound liquid and petroleum hydrocarbon contaminated soil treatment method
  • Microbial flora compound liquid and petroleum hydrocarbon contaminated soil treatment method
  • Microbial flora compound liquid and petroleum hydrocarbon contaminated soil treatment method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] Select about 120 million cfu / ml of Bacillus subtilis (CGMCC 1.821, CICC 10090) and Bacillus licheniformis (CGMCC 1.15832, CICC0037) to degrade the contaminated soil with a concentration of petroleum hydrocarbons of about 1200 mg / kg. Three samples of the test soil were collected. Concentrations of pollutants are as follows:

[0038] project pH Petroleum hydrocarbon concentration (mg / kg) Test soil 1 7.27 1193 Test soil 2 7.34 1239 Test soil 3 7.16 1232

[0039] After the three test soils were collected, air-dry them naturally to remove impurities in the soil, pass through a 5mm sieve, and adjust the water content of the test soil to about 20-25% for later use.

[0040] The component ratio of Bacillus subtilis (CGMCC 1.821, CICC 10090) and Bacillus licheniformis (CGMCC 1.15832, CICC0037) is 1:1. After mixing the separate fermentation broth of each microorganism, add clean water according to the mass ratio of 1:100 to clean water , af...

Embodiment 2

[0043] Select Bacillus subtilis (CGMCC 1.821, CICC 10090), Bacillus licheniformis (CGMCC 1.15832, CICC0037), and Brevibacterium paracondensa (CGMCC 1.838) about 120 million cfu / ml to degrade the pollution of petroleum hydrocarbon concentration of about 1200mg / kg Soil, three samples of soil for testing were collected, and the concentration of pollutants is as follows:

[0044] project pH Petroleum hydrocarbon concentration (mg / kg) Test soil 1 7.27 1193 Test soil 2 7.34 1239 Test soil 3 7.16 1232

[0045] After the three test soils were collected, air-dry them naturally to remove impurities in the soil, pass through a 5mm sieve, and adjust the water content of the test soil to about 20-25% for later use.

[0046] The component ratio of Bacillus subtilis (CGMCC 1.821, CICC 10090), Bacillus licheniformis (CGMCC 1.15832, CICC0037), and Brevibacterium paracondensa (CGMCC 1.838) is 1:1:1. After mixing the separate fermentation broth of each micr...

Embodiment 3

[0049] Select Bacillus subtilis (CGMCC 1.821, CICC 10090) and Brevibacterium paracondensa (CGMCC 1.838) with about 120 million cfu / ml to degrade the contaminated soil with a petroleum hydrocarbon concentration of about 1200 mg / kg. Three samples of the test soil were collected. Concentrations of pollutants are as follows:

[0050] project pH Petroleum hydrocarbon concentration (mg / kg) Test soil 1 7.27 1193 Test soil 2 7.34 1239 Test soil 3 7.16 1232

[0051] After the three test soils were collected, air-dry them naturally to remove impurities in the soil, pass through a 5mm sieve, and adjust the water content of the test soil to about 20-25% for later use.

[0052] The component ratio of Bacillus subtilis (CGMCC 1.821, CICC 10090) and Brevibacterium paracondensa (CGMCC 1.838) is 1:1. After mixing the separate fermentation broth of each microorganism, add clean water according to the mass ratio of 1:100 to clean water After being activate...

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Abstract

The invention provides a microbial flora compound liquid. The compound liquid comprises at least two aerobic microorganisms or facultative aerobic microorganisms. The compound liquid is formed by mixing 0.1-0.2 billion cfu/ml of a single fermentation liquid formed by fermenting each of the microorganisms according to the equal mass ratio, and the sum of the total mass percentages of all the singlefermentation liquids in the compound liquid is 100%. The microbial flora compound liquid and the petroleum hydrocarbon contaminated soil treatment method have the advantages of wide microorganism selection range, vigorous microorganism metabolism and short growth period, can produce multiple enzymes for metabolic decomposition for compounding to synergistically decompose oil hydrocarbon pollutants in the petroleum hydrocarbon-contaminated soil, and can efficiently degrade petroleum hydrocarbon components in the contaminated soil through the synergistic effect of multiple microorganisms.

Description

technical field [0001] The invention relates to a microbial flora compound liquid and a soil treatment method, in particular to a method for treating petroleum hydrocarbon-contaminated soil using a microbial flora compound liquid. Background technique [0002] Petroleum is a very important power source and industrial raw material for human beings, known as "black gold, industrial blood". Crude oil and various petroleum products enter the environment during the exploration, refining, storage, transportation and use of petroleum, causing pollution, and part of the pollution enters the soil, causing petroleum hydrocarbon pollution in the soil. Petroleum hydrocarbon pollution will seriously affect land use functions, cause changes in soil structure and properties, changes in microbial communities, decrease in soil enzyme activity, vegetation destruction and ecological variation, and lead to environmental problems such as disordered crop metabolism and serious water pollution. ...

Claims

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Application Information

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IPC IPC(8): C12N1/20B09C1/10C12R1/10C12R1/125C12R1/13C12R1/12C12R1/38
CPCC12N1/20B09C1/10
Inventor 刘勇
Owner 上海傲江生态环境科技有限公司
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