Chemiluminiscence kit for quantitatively detecting human mullerian hormone and preparation method

Abstract

The invention relates to the technical field of immunoassay, and in particular relates to a chemiluminiscence kit for quantitatively detecting human mullerian hormone. The kit comprises a human mullerian hormone AMH standard substance, a solid phase carrier coated with a human mullerian hormone AMH monoclonal antibody or polyclonal antibody, a biotin-labeled human mullerian hormone AMH monoclonalantibody or polyclonal antibody, a streptavidin-labeled enzyme substrate and a corresponding enzymatic luminescence substrate. The preparation method comprises the steps of (1) preparing the solid phase carrier coated with the human mullerian hormone AMH monoclonal antibody or polyclonal antibody; (2) preparing the biotin-labeled human mullerian hormone AMH monoclonal antibody or polyclonal antibody working solution; (3) preparing the enzyme substrate working solution marked by streptavidin; (4) preparing the standard substance; and (5) preparing a washing solution. The kit and the preparationmethod have the advantages of simplicity and convenience in operation, high operation sensitivity, good specificity, low detection cost, small sample use amount, low requirement on equipment and thelike.

Description

technical field [0001] The present invention relates to the technical field of immune analysis, in particular to a chemiluminescent reagent kit for quantitatively detecting human Mullerian hormone and a preparation method thereof. Background technique [0002] Polycystic ovary syndrome (polycysticovarysyndrome, PCOS) is one of the most common important endocrine diseases leading to ovulatory infertility in women of childbearing age. The incidence rate of PCOS is 5%-10%, and the pathogenesis is unclear. In 1947, Jost discovered that anti-Mullerian hormone (AMH) was expressed in Sertoli cells. After AMH was cloned in the 20th century, its function was gradually recognized. [0003] AMH, also known as mullerian-inhibiting substance (MIS), is a member of the transforming growth factor β (TGF-β) superfamily and is a polypeptide hormone linked by disulfide bonds. In males, AMH is secreted by testicular Sertoli cells, inhibits the development of Mullerian ducts, and participates ...

AI Technical Summary

Problems solved by technology

[0008] However, there are still many problems in the current AMH chemiluminescence detection kit, such as a large amount of samples used and high requirements for equipment, etc.

The electrochemiluminescence equipment used by Roche needs to rely on large-scale closed system equipment to power the reaction system during the detection process due to the use of magnetic beads, and the magnetic beads are easily lost due to washing and adsorption during manual operation.

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