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Method for extracting sea cucumber active substances from sea cucumber blanching liquid

A technology of active substances and blanching liquid, applied in chemical instruments and methods, ultrafiltration, membrane technology, etc., can solve the problems of simultaneous extraction, complicated steps, and increased extraction difficulty, so as to achieve high efficiency and energy saving in the extraction process, and reduce extraction costs. Effects of cost reduction and production time reduction

Pending Publication Date: 2020-11-03
HEZE BRANCH QILU UNIV OF TECH(SHANDONG ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Due to the high energy consumption and high cost of using steam in the sea cucumber blanching liquid; the use of electrodialysis desalination may easily cause the accumulation of sea cucumber lipids in the electrodialysis membrane layer, reduce the desalination efficiency, and make membrane regeneration difficult; use organic membrane ultrafiltration, interception, desalination During the operation, it is easy to breed microorganisms and the flux is low; it leads to the extraction of a single important active ingredient in the blanching liquid, and the operation steps are cumbersome, and the cost and efficiency are not ideal
[0004] For example, a variety of existing reports utilize sea cucumber blanching liquid to extract sea cucumber active substances: the CN 101343332A Chinese invention patent application published on January 14, 2009 discloses "the method for extracting sea cucumber polysaccharides by using sea cucumber processing waste liquid" , CN 102406048B Chinese invention patent application published on April 17, 2013 discloses "the method for preparing sea cucumber glycoprotein by using sea cucumber blanching liquid", CN 104402983A Chinese invention patent application published on March 11, 2015 Disclosed in the description is "the method and application of extracting glycoprotein by using sea cucumber blanching liquid", and the CN 104522728A Chinese invention patent application published on April 22, 2015 discloses "a method of extracting phospholipids and phospholipids using sea cucumber blanching liquid". The method of sea cucumber oil", these processes all extract a large class of substances in sea cucumber blanching liquid, such as sea cucumber glycoprotein, sea cucumber polysaccharide, and sea cucumber lipids, and the process steps are all multi-step extractions, which cannot be extracted at the same time. Not only the steps are complicated, but also waste other ingredients, increasing the difficulty of extraction

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] A high-efficiency and energy-saving method for extracting sea cucumber active substances from sea cucumber blanching liquid, which undergoes the following process steps:

[0024] (1) Primary filtration

[0025] The sea cucumber blanching liquid was filtered through 200 mesh to obtain 56L of primary filtrate. The sea cucumber blanching liquid is 80kg fresh sea cucumbers, which are boiled in boiling water for 15 minutes after laparotomy and removal of the intestines, and the liquid obtained after the sea cucumbers are removed; the initial filtrate has a salt content of 1.73%, an electrical conductance of 27.6ms / cm, and a solid content of 3.4%. The protein content is 7.0g / L, and the polysaccharide content is 0.12g / 100g.

[0026] (2) ultrafiltration

[0027] The initial filtrate that step (1) obtains utilizes the French Novase ceramic membrane that molecular weight is 15KDa to carry out ultrafiltration cut-off at 70 ℃, removes salt, saponin and most of moisture, obtains 4...

Embodiment 2

[0033] A high-efficiency and energy-saving method for extracting sea cucumber active substances from sea cucumber blanching liquid, which undergoes the following process steps:

[0034] (1) Primary filtration

[0035] Sea cucumber blanching liquid was selected as raw material, and 42.7L of primary filtrate was obtained by filtration. Sea cucumber blanching liquid is fresh sea cucumber 75kg laparotomed to remove the intestines, put into boiling water for 20 minutes, and the liquid obtained after removing the sea cucumber; after measurement, the salt content of the initial filtrate is 2.04%, the conductivity is 33.6ms / cm, and the solid content is 4.34%. The protein content is 8.5g / L, and the polysaccharide content is 0.14g / 100g.

[0036] (2) ultrafiltration

[0037] The primary filtrate obtained in step (1) was subjected to ultrafiltration at 75° C. with a French Novasep ceramic membrane with a molecular weight of 15 KDa to remove salt, saponin and most of the water, and obtai...

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Abstract

The invention discloses a method for extracting sea cucumber active substances from sea cucumber blanching liquid, and mainly relates to the field of sea cucumber byproduct extraction. The method comprises the following steps: S1, carrying out primary filtration on the sea cucumber blanching liquid to remove silt and particle insoluble substances, so as to obtain primary filtrate; S2, carrying outultrafiltration interception on the primary filtrate at the temperature of 60-85 DEG C by using a ceramic membrane with the molecular weight of 15 kDa to obtain a filtrate and an intercepted solution; and S3, dialyzing the trapped fluid with purified water, and drying the liquid to obtain the extract which is rich in sea cucumber polysaccharide, sea cucumber glycoprotein and sea cucumber lipid. The method can be used for simultaneously extracting the main active substances polysaccharide, glycoprotein and lipid in the sea cucumber blanching liquid by a one-step method.

Description

technical field [0001] The invention relates to the field of extracting sea cucumber by-products, in particular to a method for extracting sea cucumber active substances from sea cucumber blanching liquid. Background technique [0002] Fresh sea cucumbers contain proteolytic enzymes such as autolytic enzymes, which are easy to peel and autolyze after harvesting. In order to facilitate preservation, fresh sea cucumbers are usually processed and stored after blanching in hot water. However, during the hot water treatment of fresh sea cucumbers, a large amount of active substances such as sea cucumber glycoproteins, sea cucumber polysaccharides, sea cucumber lipids, and sea cucumber saponins will dissolve in the blanching liquid. [0003] Due to the high energy consumption and high cost of using steam in the sea cucumber blanching liquid; the use of electrodialysis desalination may easily cause the accumulation of sea cucumber lipids in the electrodialysis membrane layer, redu...

Claims

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Application Information

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IPC IPC(8): B01D61/14B01D61/20
CPCB01D61/20B01D61/146
Inventor 袁文鹏周田田许萍魏永峰侯瑾
Owner HEZE BRANCH QILU UNIV OF TECH(SHANDONG ACAD OF SCI