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Kit for detecting novel coronavirus

A coronavirus and kit technology, applied in the field of new coronavirus detection kits, can solve the problem of lack of specific, rapid and effective identification methods, and achieve the effect of accurate content

Pending Publication Date: 2020-11-03
HUNAN YONGHE YANGGUANG SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the main method used in the identification of suspected cases is to detect the total number of white blood cells and lymphocyte counts, and there is a lack of specific, rapid and effective means of identification

Method used

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  • Kit for detecting novel coronavirus
  • Kit for detecting novel coronavirus
  • Kit for detecting novel coronavirus

Examples

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preparation example Construction

[0034] In the present invention, the preparation method of the colloidal gold particle-labeled novel coronavirus recombinant protein preferably independently includes: adjusting the pH value of the colloidal gold to 7.0-9.0 with a 0.1M potassium carbonate solution, slowly adding 4 ~25μg of recombinant protein, stirred for 10~30min, then added 50~100μL of 10% BSA aqueous solution, stirred for 10~30min, centrifuged, discarded the supernatant, washed the precipitate 2~3 times with washing liquid, and used one-tenth of The initial volume of Tris-HCl buffer solution containing 1% sucrose was used to resuspend the pellet to obtain a spray solution, which was stored at 4°C for use. In the present invention, the preparation method of the IgM detection binding pad preferably includes: spraying the spray liquid onto the glass fiber and drying at 45° C. for 2 hours.

[0035] In the present invention, the preparation method of the chromatographic membrane comprises: the T line on the nitr...

Embodiment 1

[0053] Preparation of reagent strips for detecting novel coronavirus

[0054] 1. Preparation of IgM test strips

[0055] 1.1 IgM binding pad preparation

[0056] Use 0.1M potassium carbonate solution to adjust the pH of the colloidal gold to 7.0-9.0, slowly add 10 μg of novel coronavirus recombinant protein per milliliter of colloidal gold solution, stir for 10 minutes, then add 100 uL of 10% BSA aqueous solution, stir for 30 minutes, centrifuge, Discard the supernatant, wash the precipitate with washing solution for 2 to 3 times, and resuspend the precipitate with one-tenth of the initial volume of Tris-HCl buffer solution containing 1% sucrose for the last time to obtain a spray solution, and store it at 4°C for later use. The above-mentioned spraying liquid is sprayed on the glass fiber, and dried at 45°C for 2 hours.

[0057] Preparation of chromatographic membrane:

[0058] 1.1 Paste the chromatographic membrane: paste the nitrocellulose membrane of 25*300mm on the PVC...

Embodiment 2

[0070] Kit: including the test strip prepared in Example 1 and the diluent; wherein the diluent is a PBS solution containing 0.5% BSA in mass concentration, and the concentration of the PBS solution is 0.01M.

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Abstract

The invention provides a kit for detecting novel coronavirus, and belongs to the technical field of virus detection. The kit comprises a card box, an IgM detection test strip and diluent. After a patient is infected with viruses for the first time, an in-vivo immune system can be activated, IgM is generated at the initial stage of infection, the kit can be used for detecting the novel coronavirus;if the patient is infected with viruses again in the past, IgG can be generated in the body, and if the IgM detection test strip and the IgG detection test strip in the kit are used, it is possible to confirm that a patient is infected with a novel coronavirus.

Description

technical field [0001] The invention belongs to the technical field of virus detection, in particular to a kit for detecting novel coronavirus. Background technique [0002] The new coronavirus belongs to the new coronavirus of the genus β, with an envelope, and the particles are round or oval, often pleomorphic, with a diameter of 60-140nm. Its genetic characteristics are significantly different from those of SARS-CoV and MERS-CoV. The main route of transmission is through respiratory droplets, but also through contact. The incubation period is generally 3-7 days, the longest not exceeding 14 days, with fever, fatigue, and dry cough as the main manifestations. [0003] People infected with the virus will have symptoms of varying degrees, some just have a fever or a mild cough, some will develop pneumonia, and some will be more serious or even die. People of all ages can be infected, but the infection rate among children is relatively low, and it is mainly adults who are ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/68G01N33/569G01N33/558
CPCG01N33/6854G01N33/56983G01N33/558G01N2469/20
Inventor 沈林刘健余琼林戈军高诗琼靳启航陈汝彬
Owner HUNAN YONGHE YANGGUANG SCI & TECH