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A kind of elizabeth kingia anophelis 279-2 bacterial strain and its application

A technology of gdmccno and bacterial strains, applied in the direction of bacteria, biological water/sewage treatment, microorganisms, etc., can solve the problem of few types and achieve good degradation effect

Active Publication Date: 2022-04-19
SOUTH CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the types of tetracycline antibiotic-degrading microorganisms reported so far are still small, and new strain resources are urgently needed. Therefore, mining more tetracycline antibiotic-degrading bacteria has important application value

Method used

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  • A kind of elizabeth kingia anophelis 279-2 bacterial strain and its application
  • A kind of elizabeth kingia anophelis 279-2 bacterial strain and its application
  • A kind of elizabeth kingia anophelis 279-2 bacterial strain and its application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] Example 1 Isolation, purification and identification of Elizabethkingia.anophelis 279-2 strain

[0028] (1) Separation and purification: In July 2017, 0.1 mL of fresh samples were drawn from Qinghai migratory bird feces samples and added to 1 mL of 0.9% normal saline, and vortexed to mix. Pipette 20uL of the suspension into 4mL LB broth and incubate at 30°C and 180rpm for 12-16 hours. Subsequently, the above-mentioned culture solution was divided into LB agar medium containing 64ug / mL tetracycline, and cultured at 30° C. for 16-24 hours. Pick the purified single colony, use the same LB agar medium for further purification, and culture at 30°C for 16-24 hours to obtain a purified single colony.

[0029] (2) Identification of 16S rDNA strains: Scrape a ring of the above-mentioned purified single colony, and extract the genome of the strain according to the instructions of the Tiangen Bacteria Genomic DNA Extraction Kit (Tiangen Biochemical Technology Co., Ltd.). Subsequ...

Embodiment 2

[0031]Example 2 Colony Morphology and Physiological and Biochemical Characteristics of Elizabethkingia.anophelis 279-2 Strain

[0032] Elizabethkingia.anophelis 279-2 is a gram-negative bacterium, obligate to aerobic, the optimum growth temperature is 35°C, the nutritional requirements are not high, it can grow on ordinary medium, but it grows weakly or not at all on MacConkey medium .

Embodiment 3

[0033] Example 3 Deposit of Elizabethkingia.anophelis 279-2

[0034] The deposit information of Elizabethkingia.anophelis 279-2 is as follows:

[0035] Preservation time: July 23, 2020;

[0036] Name of depository unit: Guangdong Microbial Culture Collection Center (GDMCC);

[0037] Deposit number: GDMCC No: 61096;

[0038] Address of Preservation Unit: 5th Floor, Building 59, Compound, No. 100 Xianlie Middle Road, Guangzhou;

[0039] Taxonomic designation: Elizabethkingia.anophelis.

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Abstract

The invention belongs to the technical field of biodegradation of antibiotics, and discloses an Elizabethkingia.anophelis 279-2 strain and its application in degrading tetracycline antibiotics. The invention provides an Elizabethkingia.anophelis 279-2 strain, which was released in 2020 It was preserved in Guangdong Microbial Culture Collection Center on July 23, and the preservation number is GDMCC No: 61096. The 16S rDNA of this strain has the nucleotide sequence shown in SEQ ID NO: 3. Doxycycline, minocycline and ibaracycline have a good degradation effect, and can degrade 64.2% of ibaracycline after 16 hours, which is doxycycline, minocycline and ibaracycline The problem of varacycline residues provides a safe and environmentally friendly solution.

Description

technical field [0001] The invention relates to the technical field of microbial remediation, and more specifically relates to an Elizabethkingia.anophelis 279-2 bacterial strain and its application. Background technique [0002] Tetracycline antibiotics are a class of broad-spectrum antibiotics produced by actinomycetes. Their structures all contain naphthacene basic skeletons. The main types include tetracycline, oxytetracycline, aureomycin, doxycycline, rice Nocycline, tigecycline, and ilavacycline. Doxycycline, minocycline and ibaracycline, which are tetracycline antibiotics, are widely used in various countries in the world due to their low cost, convenient use, and wide antibacterial spectrum, and are widely used in the prevention and treatment of livestock and poultry diseases. Treatment and promotion of livestock and poultry growth. At present, tetracycline antibiotics are one of the most widely used antibiotics in my country. According to statistics, the amount us...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C02F3/34B09C1/10C12R1/01C02F101/38
CPCC02F3/34B09C1/10C02F2101/38C12R2001/01C12N1/205
Inventor 孙坚唐甜崔超月崔泽华陈冲廖晓萍刘雅红
Owner SOUTH CHINA AGRI UNIV
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