Micro-liquid droplet double-fluorescence signal detection device
A technology of signal detection and detection device, which is applied in measurement devices, fluorescence/phosphorescence, material analysis by optical means, etc. Influence and other problems, to achieve the effect of beating improvement, rich detection results, and improved detection efficiency
Pending Publication Date: 2020-11-06
TARGETINGONE TECH (BEIJING) CORP
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Problems solved by technology
The laser confocal detection method has the characteristics of high resolution and high sensitivity, and can obtain clear digital fluorescent images and quantitative analysis results of fluorescently labeled antibodies on the biochip, and can become a new detection method mainly used in high-density biochip scanning , but the current laser confocal fluorescence detection signal devices all use a single fluorescence detection signal, and the detection signal acquisition and corresponding result acquisition are relatively single, and the single detection result acquisition has relatively large limitations, and the applicable scenarios and fields are relatively narrow. higher cost
At the same time, the relative movement between the modules in the optical path of the detection device will also have a certain impact on the accuracy of the detection results
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[0037]The light processing module 2 includes a first photomultiplier tube (PMT) 22 for acquiring the first fluorescence, a second photomultiplier tube 21 for acquiring the second fluorescence, and is used to reflect the mixed excitation light to the objective lens by the optical combination module A first dichroic mirror 261 of 25, and a second dichroic mirror 289 separating the first fluorescent light from the second fluorescent light;
[0038] Wherein, the optical path module includes an optical combining module 1 and an optical processing module 2;
[0039] The overall mobile module 3 is used to move the optical path module as a whole to make the mixed laser confocal on the micro-measurement droplet to be detected, and the optical path connecting piece 33 is connected to the screw holes on the first connecting piece 26 and the second connecting piece 27, and then It is connected with the slider fixing frame 32, so that the motor module 31 can drive the optical path module t...
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The invention relates to a micro-liquid droplet double-fluorescence signal detection device, which comprises a light combination module, an objective lens and a light processing module, and is characterized by further comprising an integral moving module, wherein the integral moving module is connected with the light processing module and is arranged to integrally move a light path module formed by the light combination module and the light processing module so as to enable mixed laser to be confocal on to-be-detected micro-liquid droplets. According to the invention, the micro-droplet double-fluorescence signal integral movement detection device can integrally move to obtain a double-fluorescence detection signal of a micro-droplet through aa light combination module and a light processing module, so that the straightness, the motion precision and the bounce of the motion mechanism are greatly improved; the relative position relationship between an objective lens and a diaphragm surface is fixed to ensure that the image of a focal plane is relatively fixed on the diaphragm surface; and acquisition focus is searched through an overall moving light path, and the purpose of stable and consistent signal acquisition is achieved.
Description
technical field [0001] The invention relates to the technical field of microfluidic chips, in particular to a device for detecting overall movement of micro-droplets with dual fluorescent signals. Background technique [0002] Through the miniaturization and integration of chemical analysis equipment, microfluidic chip technology maximizes the integration of the functions of the analysis laboratory into portable equipment, even on a chip with a small size. However, the overall performance of the detector will affect the entire The detection limit, detection speed, scope of application and volume of the microfluidic chip analysis system are a key part of the microfluidic chip analysis system. A focus and hotspot. Many detection methods based on different detection principles have been used in the research of microfluidic chip analysis. Microfluidic chip detectors are generally classified according to their principles, which can be roughly divided into optical detectors and el...
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Login to View More IPC IPC(8): G01N21/64G01N21/01
CPCG01N21/01G01N21/64G01N21/6402G01N21/645G01N21/6458G01N21/6486G01N2021/0112G01N2021/6419G01N2021/6471G01N2021/6478
Inventor周跃夏雷梁欢迎于海侠黄海旺白宇杨文军
OwnerTARGETINGONE TECH (BEIJING) CORP