Tumor precise and personalized drug treatment method and application

A treatment method and anti-tumor drug technology, applied in the field of precision treatment, can solve the problems of time-consuming, easy contamination of samples, low sensitivity, etc., achieve good genetic phenotype and heterogeneity, avoid toxic and side effects, and improve the success rate.

Pending Publication Date: 2020-11-06
DALIAN INST OF CHEM PHYSICS CHINESE ACAD OF SCI
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  • Abstract
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  • Claims
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Problems solved by technology

It also solves the problems of traditional culture methods such as long time-consuming, easy contamination of samples, and low sensitivity, and provides the possibility for the development of drug sensitivity experiments for clinical tumor unidirectional drug use and combined drug use.

Method used

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  • Tumor precise and personalized drug treatment method and application
  • Tumor precise and personalized drug treatment method and application
  • Tumor precise and personalized drug treatment method and application

Examples

Experimental program
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Effect test

Embodiment 1

[0040] The tumor tissues isolated from patients with solid tumor tumors (breast cancer) can be processed in a sterile environment in the shortest time. Cut the tumor sample from the non-necrotic site, and immediately immerse the sample in a sterile vial containing PBS pre-cooled at 4°C, and send it to the laboratory within 3 hours. Among them, PBS contains 100U / mL penicillin and 100U / mL streptomycin; in a biological safety cabinet, transfer the tumor sample to a 100mm cell culture dish, rinse with 1×PBS 10mL three times, wash off the blood cells, and peel off the surface of the tumor specimen Connective tissue, fiber and fat, and prepare the tumor tissue into a diameter less than 1mm 3 The tumor microstructure such as figure 1 Shown in a. The cell culture medium is configured with 10% patient serum and 90% DMEM. The tumor micro-tissue is cultured on the chip, placed at 37 degrees, 5% CO 2 , 5% O 2 Cultivated in an environment such as figure 1 Shown in b. To stimulate the tumor...

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Abstract

The invention relates to a tumor precise and personalized drug treatment method and application, in particular to a method for researching tumors, evaluating the drug effect of an anti-tumor drug, testing the drug effect sensitivity of the anti-tumor drug and screening an anti-tumor treatment scheme by culturing tumor tissues of a patient in vitro. Proper primary tumor samples are selected and cultured in a microenvironment simulating growth of in-vivo sample tumors, so that drug testing can be performed quickly. According to the method, the problems of long time consumption and poor data correlation of tumor personalized treatment scheme screening and antitumor drug evaluation are solved, the in-vivo real environment is better met under the hypoxic culture condition, meanwhile, the used tumor tissue is not digested and dispersed in vitro, the components and characters of the tumor tissue are reserved, and the efficacy of the tumor tissue is ensured. In addition, the consumption of samples and reagents is remarkably reduced, a plurality of experiment related parameters can be obtained at the same time at a time, and expected data can be obtained within 5 days.

Description

Technical field [0001] The present invention relates to the technical field of precision treatment, in particular to a method for culturing tumor models in vitro and using the tumor models to study tumors, evaluate the efficacy of anti-tumor drugs, test the sensitivity of anti-tumor drugs, and screen anti-tumor treatment plans . Background technique [0002] At present, for most different patients suffering from the same disease, the treatment method is to use the same medicine and standard dosage, but in fact, different patients have great differences in the treatment effects and adverse reactions. Sometimes this difference can even be fatal. . Commonly used anti-tumor chemotherapy drugs are less than 70% effective in treating patients, and about 20%-35% of patients have received inappropriate drug treatment. If tumor treatment can treat the same disease with different treatments, different from person to person, and implement individualized treatment, it will greatly improve ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/50
CPCG01N33/5011G01N33/5017G01N33/5088G01N2500/10
Inventor 秦建华李中玉
Owner DALIAN INST OF CHEM PHYSICS CHINESE ACAD OF SCI
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