Multi-purpose method of serratia marcescens strains
A technology of Serratia marcescens and Serratia marcescens, which is applied in the field of fermentation engineering to achieve the effects of easy purification and strong specificity
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Embodiment 1
[0033] A multi-purpose method for Serratia marcescens strain, comprising steps:
[0034] 1) Preparation of strain seed solution
[0035] Serratia marcescens ( Serratia marcescens ) SDSPY-136 strain was transferred to the slant medium for activation. The components of the slant medium were: peptone 5 g / L, sodium chloride 5 g / L, beef extract 3 g / L, agar 20 g / L, pH Value 7.0.
[0036] 250mL Erlenmeyer flask is filled with 30mL liquid seed culture medium, the components of liquid seed culture medium are: peptone 5 g / L, sodium chloride 5 g / L, beef extract 3 g / L, pH value 7.0. Steam sterilization at 121℃ 20min, cool to room temperature, inoculate 2 rings of slant strains, place on a shaker with a rotation speed of 200rpm and a rotation radius of 40mm, and cultivate at 30°C for 12h, OD 600 It was 0.267, and it was fermented as a seed liquid.
[0037] 2) Preparation of 2-keto-D-gluconic acid
[0038] A 5L fermenter was used to prepare the fermentation medium according to the liq...
Embodiment 2
[0053] A multi-purpose method for Serratia marcescens strain, comprising steps:
[0054] 1) Preparation of strain seed solution
[0055] The Serratia marcescens SDSPY-136 strain was transferred to the slant medium for activation. The components of the slant medium were: peptone 5 g / L, sodium chloride 5 g / L, beef extract 3 g / L, agar 20 g / L, pH 7.0.
[0056] 250mL Erlenmeyer flask is filled with 30mL liquid seed culture medium, the components of liquid seed culture medium are: peptone 5 g / L, sodium chloride 5 g / L, beef extract 3 g / L, pH value 7.0. Steam sterilization at 121℃ 20min, cool to room temperature, inoculate 2 rings of slant strains, place on a shaker with a rotation speed of 200rpm and a rotation radius of 40mm, and cultivate at 30°C for 12h, OD 600 It was 0.267, and it was fermented as a seed liquid.
[0057] 2) Preparation of 2-keto-D-gluconic acid
[0058] 250mL Erlenmeyer flask is equipped with 30mL 2-KGA fermentation medium, the components of 2-KGA fermentatio...
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