A method for efficiently producing water-soluble β-1,3-glucan by fermentation of golden algae

Abstract

The present invention relates to a method for efficiently producing water-soluble β-1,3-glucan through the fermentation of golden algae. Chrysophylla species of water-soluble β‑1,3‑glucan; it includes inoculating Chrysophylla species in the fermentation basal medium, adding feeding medium during the fermentation process; by regulating the type of nitrogen source in the fermentation medium, the fermentation medium The C / N ratio and the glucose concentration in the fermentation medium are maintained within a certain range to regulate the growth rate and cell density of the golden algae cells, so as to obtain the golden algae fermentation broth with high biomass concentration and high cell density, thereby improving the growth rate of golden algae. Production of β-1,3-glucan in cell water solubility. On the one hand, the present invention solves the problems of low content of β-1,3-glucan in existing β-1,3-glucan sources, high extraction cost, and mostly water-insoluble; Inhibition of cell growth when increasing the content of storage polysaccharides in microalgal cells.

Description

technical field [0001] The invention belongs to the field of microalgae cultivation, and relates to a method for efficiently producing water-soluble β-1,3-glucan through golden algae fermentation. Background technique [0002] β-1,3-glucan is a kind of macromolecular polysaccharide widely present in microorganisms, plants and animals. The main chain structure is connected by β-1,3-glucosidic bonds, and usually contains different proportions and sizes due to different sources Branched chains connected by β-1,3, β-1,4 or β-1,6 bonds mainly exist in the form of cellular structural components such as cell walls. β-1,3-glucan is a high-efficiency biological responder with strong activity and low toxic and side effects. It not only has immune-stimulating effect, but also has anti-tumor and anti-infection activities. In the feed industry, water-soluble yeast β-1,3-glucan can be used as a feed additive, which can improve the non-specific and specific immunity of animals. At presen...

AI Technical Summary

Problems solved by technology

In terms of increasing the polysaccharide content of microalgae cells, the existing methods of nitrogen limitation or nitrogen starvation induction increase the content of storage polysaccharides (such as starch) in microalgae cells, and the cell growth is inhibited, and the polysaccharide production is difficult to increase, which is not suitable for industrial production.

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